Product Info Summary
SKU: | PA2175 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Tuberin/TSC2 Antibody
SKU/Catalog Number
PA2175
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tuberin/TSC2 Antibody catalog # PA2175. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tuberin/TSC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2175)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Tuberin, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2175 is reactive to TSC2 in Human, Mouse, Rat
Applications
PA2175 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
200 kDa
Calculated molecular weight
200.608kDa
Background of TSC2
Tuberous sclerosis 2 protein, also known as TSC2 or Tuberin is a protein that is in humans. The chromosomal location of this gene is 16p13.3. Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. This gene involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. It stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-Tuberin antibody, PA2175, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: HEPA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For TSC2 (Source: Uniprot.org, NCBI)
Gene Name
TSC2
Full Name
Tuberin
Weight
200.608kDa
Alternative Names
LAM; LAMFLJ43106; TSC2; TSC4; Tuberin; Tuberous sclerosis 2 protein; tuberous sclerosis 2 TSC2 LAM, PPP1R160, TSC4 TSC complex subunit 2 tuberin|protein phosphatase 1, regulatory subunit 160|tuberous sclerosis 2 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSC2, check out the TSC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tuberin/TSC2 Antibody (PA2175)
Hello CJ!
No publications found for PA2175
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Tuberin/TSC2 Antibody
Question
Do you have a BSA free version of anti-Tuberin/TSC2 antibody PA2175 available?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Tuberin/TSC2 antibody PA2175 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-23
Question
Does PA2175 anti-Tuberin/TSC2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
It shows on the product datasheet, PA2175 anti-Tuberin/TSC2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-27
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-Tuberin/TSC2 antibody PA2175. Let me know if you need anything else.
C. Krishna
Verified customer
Asked: 2018-09-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-13
Question
My question regards to test anti-Tuberin/TSC2 antibody PA2175 on human leukemic t-cell for research purposes, then I may be interested in using anti-Tuberin/TSC2 antibody PA2175 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Gonzalez
Verified customer
Asked: 2018-09-06
Answer
The products we sell, including anti-Tuberin/TSC2 antibody PA2175, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-06
Question
I was wanting to use your anti-Tuberin/TSC2 antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
You can see on the product datasheet, PA2175 anti-Tuberin/TSC2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-11-21
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-Tuberin/TSC2 antibody PA2175. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-28