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SKU:MA1100
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:TH-16
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)
SKU/Catalog Number MA1100
Description Mouse IgG monoclonal antibody for Tyrosine Hydroxylase, tyrosine hydroxylase (TH) detection. Tested with WB, IHC-P, IHC-F in Human;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) TH-16 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1100)
Replacement Item This antibody may replace the following items: sc-47708|sc-7848|sc-25269|sc-7847|sc-374048|sc-374047|sc-73151|sc-73152|sc-14007|sc-136100 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Rabbit, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Rat tyrosine hydroxylase(TH).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number TH-16
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Th
Protein Name Tyrosine 3-monooxygenase
Molecular Weight 55966 MW
Protein Function Plays an important role in the physiology of adrenergic neurons.
Tissue Specificity TH: Mainly expressed in the brain and adrenalglands.
Sequence Similarities Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Uniprot ID P04177
Alternative Names Tyrosine 3-monooxygenase;1.14.16.2;Tyrosine 3-hydroxylase;TH;Th;
Research Areas TH|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tyrosine 3-monooxygenase

Tyrosine hydroxylase is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Human TH gene contains 13 primary exons and spans approximately 8 kb. TH is in the 11p15.5 region.

Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) Images

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Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)
Figure 1. IHC analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MA1100).
Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Tyrosine Hydroxylase Antibody (MA1100) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16)
Figure 2. Western blot analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MA1100).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Tyrosine Hydroxylase antigen affinity purified polyclonal antibody (Catalog # MA1100) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase at approximately 59KD. The expected band size for Tyrosine Hydroxylase is at 59KD.
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Publications

Hu W, Ye Y, Wang J, Zhang W, Chen A, Guo F. Artif Cells Nanomed Biotechnol. 2013 Aug;41(4):249-54. Doi: 10.3109/10731199.2012.731412. Epub 2013 Jan 10. Bone Morphogenetic Proteins Induce Rabbit Bone Marrow-Derived Mesenchyme Stem Cells To Differen...
Li X, Jiang Yh, Jiang P, Yang Jl, Ma Df, Yang Ch. Chin J Integr Med. 2014 Jul;20(7):524-33. Doi: 10.1007/S11655-014-1861-Z. Epub 2014 Jun 28. Effect Of Guizhi Decoction ([Symbols; See Text]) On Heart Rate Variability And Regulation Of Cardiac Auto...
Su Y, Fu Y, Zhang H, Shi Z, Zhang J, Gao L. Fish Physiol Biochem. 2015 Oct;41(5):1093-104. Doi: 10.1007/S10695-015-0071-8. Epub 2015 Jun 3. Identification And Expression Of Srf Targeted By Mir-133A During Early Development Of Paralichthys Olivaceus.
Deng Y, Jiao C, Mi C, Xu B, Li Y, Wang F, Liu W, Xu Z. Mol Neurobiol. 2015 Feb;51(1):68-88. Doi: 10.1007/S12035-014-8789-3. Epub 2014 Jun 28. Melatonin Inhibits Manganese-Induced Motor Dysfunction And Neuronal Loss In Mice: Involvement Of Oxidativ...
Wu L, Zhao Q, Zhu X, Peng M, Jia C, Wu W, Zheng J, Wu Xz. Brain Pathol. 2010 Nov;20(6):1042-54. Doi: 10.1111/J.1750-3639.2010.00410.X. Epub 2010 Jun 14. A Novel Function Of Microrna Let-7D In Regulation Of Galectin-3 Expression In Attention Defici...
Wan C, Liu Nn, Liu Lm, Cai N, Chen L. Int J Ophthalmol. 2010;3(2):145-8. Doi: 10.3980/J.Issn.2222-3959.2010.02.12. Epub 2010 Jun 18. Effect Of Adenovirus-Mediated Brain Derived Neurotrophic Factor In Early Retinal Neuropathy Of Diabetes In Rats.
Zhao J, Cheng Yy, Fan W, Yang Cb, Ye Sf, Cui W, Wei W, Lao Lx, Cai J, Han Yf, Rong Jh. Cns Neurosci Ther. 2015 Jan;21(1):61-70. Doi: 10.1111/Cns.12334. Epub 2014 Oct 14. Botanical Drug Puerarin Coordinates With Nerve Growth Factor In The Regulatio...
Wu Y, Ge C, Zeng W, Zhang C. Stem Cells Dev. 2010 Feb;19(2):195-202. Doi: 10.1089/Scd.2008.0383. Induced Multilineage Differentiation Of Chicken Embryonic Germ Cells Via Embryoid Body Formation.
Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tyrosine hydroxylase antibody, th antibody