Anti-Phospho-Tyrosine Hydroxylase (Ser40) Antibody

Tyrosine Hydroxylase antibody

Boster Bio Anti-Phospho-Tyrosine Hydroxylase (Ser40) Antibody (Catalog # P01917-1). Tested in WB, IHC, ICC applications. This antibody reacts with Many Mammalian, many Non-mammalian.

Product Info Summary

SKU: P01917-1
Size: 100 µl
Reactive Species: Mammals, Non-mammals
Host: Rabbit
Application: IHC, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-Phospho-Tyrosine Hydroxylase (Ser40) Antibody

View all Tyrosine Hydroxylase Antibodies

SKU/Catalog Number

P01917-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Tyrosine Hydroxylase (Ser40) Antibody (Catalog # P01917-1). Tested in WB, IHC, ICC applications. This antibody reacts with Many Mammalian, many Non-mammalian.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Tyrosine Hydroxylase (Ser40) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01917-1)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

608

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P01917-1 is reactive to TH in Mammals, Non-mammals

Applications

P01917-1 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

58.6kDa

Background of Tyrosine Hydroxylase

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC: 1:1000
ICC: 1:50-1:1000

Validation Images & Assay Conditions

Gene/Protein Information For TH (Source: Uniprot.org, NCBI)

Gene Name

TH

Full Name

Tyrosine 3-monooxygenase

Weight

58.6kDa

Superfamily

biopterin-dependent aromatic amino acid hydroxylase family

Alternative Names

TH; DYT14; DYT5b; EC 1.14.16; EC 1.14.16.2; TH mouse; TH; TYH dystonia 14; TYH; Tyrosine 3-hydroxylase; tyrosine 3-monooxygenase; Tyrosine Hydroxylase immunohistochemistry; Tyrosine Hydroxylase mouse; Tyrosine Hydroxylase TH DYT14, DYT5b, TYH tyrosine hydroxylase tyrosine 3-monooxygenase|dystonia 14|tyrosine 3-hydroxylase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TH, check out the TH Infographic

TH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for P01917-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Phospho-Tyrosine Hydroxylase (Ser40) Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Phospho-Tyrosine Hydroxylase (Ser40) Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

13 Customer Q&As for Anti-Phospho-Tyrosine Hydroxylase (Ser40) Antibody

Question

Will P01917-1 anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

You can see on the product datasheet, P01917-1 anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-27

Question

I see that the anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-15

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-15

Question

I was wanting to use your anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody for IF for all animals substantia nigra on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for all animals substantia nigra identification?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

It shows on the product datasheet, P01917-1 anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody has been tested for IF, IHC, WB on all animals tissues. We have an innovator award program that if you test this antibody and show it works in all animals substantia nigra in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-28

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for substantia nigra using anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-10

Question

Can you help my question with product P01917-1, anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P01917-1 anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-14

Question

Is a blocking peptide available for product anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody (P01917-1)?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

We do provide the blocking peptide for product anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody (P01917-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-01

Question

Does anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 work for IF with substantia nigra?

H. Baker

Verified customer

Asked: 2019-01-30

Answer

According to the expression profile of substantia nigra, TH is highly expressed in substantia nigra. So, it is likely that anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 will work for IF with substantia nigra.

Boster Scientific Support

Answered: 2019-01-30

Question

We are currently using anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 for all animals tissue, and we are content with the WB results. The species of reactivity given in the datasheet says all animals. Is it likely that the antibody can work on feline tissues as well?

T. Parker

Verified customer

Asked: 2018-09-27

Answer

The anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody (P01917-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-09-27

Question

I would like to test anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 on all animals substantia nigra for research purposes, then I may be interested in using anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-06-05

Answer

The products we sell, including anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-06-05

Question

Is this P01917-1 anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody reactive to the isotypes of TH?

Verified Customer

Verified customer

Asked: 2018-02-15

Answer

The immunogen of P01917-1 anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-02-15

Question

Do you have a BSA free version of anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 available?

Verified Customer

Verified customer

Asked: 2017-05-11

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-05-11

Question

Would anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 work on feline IHC with substantia nigra?

K. Jones

Verified customer

Asked: 2015-08-06

Answer

Our lab technicians have not validated anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline substantia nigra in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-08-06

Question

I have attached the WB image, lot number and protocol we used for substantia nigra using anti-Phospho-Tyrosine Hydroxylase (Ser40) antibody P01917-1. Please let me know if you require anything else.

N. Rodriguez

Verified customer

Asked: 2014-10-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-27

Order DetailsPrice
P01917-1

100uL

$465

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
P01917-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$465.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.