Data & Images
|Product Name||Anti-UBE2I/UBC9 Antibody|
|Description||Rabbit IgG polyclonal antibody for SUMO-conjugating enzyme UBC9(UBE2I) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-UBE2I/UBC9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2258)|
|Replacement Item||This antibody may replace the following items: sc-10759|sc-130281|sc-136245|sc-271057|sc-5231 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human UBE2I(108-122aa IKQILLGIQELLNE), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||SUMO-conjugating enzyme UBC9|
|Molecular Weight||18007 MW|
|Protein Function||Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. .|
|Tissue Specificity||Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. .|
|Sequence Similarities||Belongs to the ubiquitin-conjugating enzyme family.|
|Subcellular Localization||Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity). .|
|Alternative Names||SUMO-conjugating enzyme UBC9;6.3.2.-;SUMO-protein ligase;Ubiquitin carrier protein 9;Ubiquitin carrier protein I;Ubiquitin-conjugating enzyme E2 I;Ubiquitin-protein ligase I;p18;UBE2I;UBC9, UBCE9;|
|Research Areas|||cell biology|proteolysis / ubiquitin|proteasome / ubiquitin|sumo| epigenetics and nuclear signaling|chip'ing antibodies|ubiquitin & ubiquitin like modifiers| metabolism|types of disease|obesity| cell biology|ubiquitin e2 enzymes||
Background for SUMO-conjugating enzyme UBC9
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-UBE2I/UBC9 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-UBE2I/UBC9 Antibody Images
Click the images to enlarge.
All lanes: Anti-UBE2I(PA2258) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 40ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 3: Rat Pancreas Tissue Lysate at 40ug
Predicted bind size: 18KD
Observed bind size: 18KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,