Show Order Info
SKU:PA1981
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:WB
Price: $200.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-UCP1 Antibody
SKU/Catalog Number PA1981
Description Rabbit IgG polyclonal antibody for Mitochondrial brown fat uncoupling protein 1(UCP1) detection. Tested with WB in Human.
Cite This Product Anti-UCP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1981)
Replacement Item This antibody may replace the following items: sc-6529|sc-6528 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human UCP1(289-307aa FEQLKRELSKSRQTMDCAT).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name UCP1
Protein Name Mitochondrial brown fat uncoupling protein 1
Molecular Weight 33005 MW
Protein Function UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
Tissue Specificity Brown adipose tissue. .
Sequence Similarities Belongs to the mitochondrial carrier (TC 2.A.29) family.
Subcellular Localization Mitochondrion inner membrane; Multi-pass membrane protein.
Uniprot ID P25874
Alternative Names Mitochondrial brown fat uncoupling protein 1;UCP 1;Solute carrier family 25 member 7;Thermogenin;UCP1;SLC25A7, UCP;
Research Areas |signal transduction|metabolism|energy metabolism| signal transduction|mitochondrial| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|metabolic signaling pathways|lipid and lipoprotein metabolism|lipid metabolism|energy transfer pathways|types of disease|obesity|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Mitochondrial brown fat uncoupling protein 1

UCP1 (Uncoupling Protein 1), also called THERMOGENIN or UCP, is an uncoupling protein found in the mitochondria of brown adipose tissue (BAT). Using in situ hybridization, the human UCP gene is assigned to 4q31. Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

Anti-UCP1 Antibody Images

Click the images to enlarge.

Anti-UCP1 Antibody
Anti-UCP1 antibody, PA1981, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human UCP1,30.0KD (162aa tag+ K199-T307)
Lane 1: Recombinant Human UCP1 Protein 10ng
Lane 2: Recombinant Human UCP1 Protein 5ng
Lane 3: Recombinant Human UCP1 Protein 2.5ng
Lane 4: Recombinant Human UCP1 Protein 1.25ng
Write a review for PA1981

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to ucp1 antibody, ucp 1 antibody, uncoupling protein 1 antibody