|Validated Species:||Mouse, Rat|
Data & Images
|Product Name||Anti-UCP1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Mitochondrial brown fat uncoupling protein 1(UCP1) detection. Tested with WB in Mouse;Rat.|
|Cite This Product||Anti-UCP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1982)|
|Replacement Item||This antibody may replace the following items: sc-6529|sc-6528 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse UCP1(289-307aa FEQLKKELMKSRQTVDCTT), identical to the related rat sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Mitochondrial brown fat uncoupling protein 1|
|Molecular Weight||33212 MW|
|Protein Function||UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.|
|Tissue Specificity||Brown adipose tissue.|
|Sequence Similarities||Belongs to the mitochondrial carrier (TC 2.A.29) family.|
|Subcellular Localization||Mitochondrion inner membrane; Multi-pass membrane protein.|
|Alternative Names||Mitochondrial brown fat uncoupling protein 1;UCP 1;Solute carrier family 25 member 7;Thermogenin;Ucp1;Slc25a7, Ucp;|
|Research Areas|||signal transduction|metabolism|energy metabolism| signal transduction|mitochondrial| cancer|cancer metabolism|metabolic signaling pathway|metabolism of lipids and lipoproteins| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|metabolic signaling pathways|lipid and lipoprotein metabolism|lipid metabolism|energy transfer pathways|types of disease|obesity||
Background for Mitochondrial brown fat uncoupling protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-UCP1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Mouse, Rat |
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-UCP1 Antibody Images
Click the images to enlarge.
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Mouse Spleen Tissue Lysate
Lane 4: Mouse Lung Tissue Lysate
Lane 5: HEPA Cell Lysate
- Review by Aijun Qiao
"Good UCP1 Antibody"
--Aijun Qiao, CANCER CENTER, AUGUSTA UNIVERSITY, POSTDOCTORAL FELLOWS
Applications Western Blot Sample Subcutaneous fat and brown fat tissue Detection Western Blot
"We looked at the thermogenesis levels of our KO mice using this antibody which is molecular marker for heat production to see if our KO has high level of heat production to protect from obesity. We can get a clear band of UCP1 in subcutaneous fat and brown fat tissue which are both responsible for thermogenesis. Although there are some non-specific bands, we still can get the strong and clear bands that we want to. The WB band is strong even when you use high dilution concentration. Overall this antibody is good to check UCP1 levels using WB technology."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,