Anti-UNC45A Antibody (Monoclonal, AbS1)

UNC45A antibody

Boster Bio Anti-UNC45A Antibody (Monoclonal, AbS1) catalog # M08608. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M08608
Size: 50μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: WB

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Product Name

Anti-UNC45A Antibody (Monoclonal, AbS1)

View all UNC45A Antibodies

SKU/Catalog Number

M08608

Size

50μg/vial

Form

Liquid

Description

Boster Bio Anti-UNC45A Antibody (Monoclonal, AbS1) catalog # M08608. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-UNC45A Antibody (Monoclonal, AbS1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08608)

Host

Mouse

Contents

Each vial contains 50% glycerol and 0.09% sodium azide.

Clonality

Monoclonal

Clone Number

AbS1

Isotype

IgG1

Immunogen

Recombinant human UNC45.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M08608 is reactive to UNC45A in Human, Mouse, Rat

Applications

M08608 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

103.077kDa

Background of UNC45A

Heat shock protein 47, also known as SERPINH1 or HSP47, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, when absent, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:1, 000, colorimetric, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For UNC45A (Source: Uniprot.org, NCBI)

Gene Name

UNC45A

Full Name

Protein unc-45 homolog A

Weight

103.077kDa

Alternative Names

FLJ10043; GCUNC45; GC-UNC45; GCUNC-45; general cell UNC45; protein unc-45 homolog A; SMAP-1IRO039700; SMAP1UNC-45A; smooth muscle cell associated protein-1; Smooth muscle cell-associated protein 1; unc-45 homolog A (C. elegans); Unc-45A UNC45A GC-UNC45, GCUNC-45, GCUNC45, IRO039700, SMAP-1, SMAP1, UNC-45A unc-45 myosin chaperone A protein unc-45 homolog A|general cell UNC45|smooth muscle cell associated protein-1|unc-45 homolog A

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on UNC45A, check out the UNC45A Infographic

UNC45A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for UNC45A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-UNC45A Antibody (Monoclonal, AbS1)

Question

Will anti-UNC45A antibody (Monoclonal, AbS1) M08608 work on zebrafish WB with lung uterus?

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

Our lab technicians have not tested anti-UNC45A antibody (Monoclonal, AbS1) M08608 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-UNC45A antibody (Monoclonal, AbS1) M08608 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish lung uterus in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-11

Question

We are currently using anti-UNC45A antibody (Monoclonal, AbS1) M08608 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

The anti-UNC45A antibody (Monoclonal, AbS1) (M08608) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-12

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-UNC45A antibody (Monoclonal, AbS1) M08608. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-10-22

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-22

Question

Is this M08608 anti-UNC45A antibody (Monoclonal, AbS1) reactive to the isotypes of UNC45A?

Verified Customer

Verified customer

Asked: 2017-11-13

Answer

The immunogen of M08608 anti-UNC45A antibody (Monoclonal, AbS1) is Recombinant human UNC45. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-13

Question

I see that the anti-UNC45A antibody (Monoclonal, AbS1) M08608 works with WB, what is the protocol used to produce the result images on the product page?

M. Mangal

Verified customer

Asked: 2017-05-01

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-05-01

Order DetailsPrice
M08608

200ug

$297

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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