|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-uPAR Antibody|
|Description||Rabbit IgG polyclonal antibody for Urokinase plasminogen activator surface receptor(PLAUR) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-uPAR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1344)|
|Replacement Item||This antibody may replace the following items: sc-10815|sc-13522|sc-135296|sc-137591|sc-137593|sc-168516|sc-168518|sc-243373|sc-243375|sc-243376|sc-243378|sc-247664|sc-247665|sc-247743|sc-247744|sc-247913|sc-247915|sc-247919|sc-247921|sc-376118|sc-376494|sc-87285|sc-87286|sc-9791|sc-9793|sc-98139|sc-98140 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human uPA Receptor(293-304aa CNHPDLDVQYRS).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Urokinase plasminogen activator surface receptor(U-PAR/uPAR)|
|Molecular Weight||36978 MW|
|Protein Function||Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.|
|Tissue Specificity||Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.|
|Sequence Similarities||Contains 3 UPAR/Ly6 domains.|
|Subcellular Localization||Cell membrane . Cell projection, invadopodium membrane . Colocalized with FAP (seprase) preferentially at the cell surface of invadopodia membrane in a cytoskeleton-, integrin- and vitronectin-dependent manner. .|
|Alternative Names||Urokinase plasminogen activator surface receptor;U-PAR;uPAR;Monocyte activation antigen Mo3;CD87;PLAUR;MO3, UPAR;|
|Research Areas|||cardiovascular|blood|fibrinolysis / thrombolysis| immunology|cell type markers|myeloid cells| cancer|invasion/microenvironment|ecm|extracellular matrix|tumor biomarkers|receptors||
Background for Urokinase plasminogen activator surface receptor(U-PAR/uPAR)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-uPAR Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
Anti-uPAR Antibody Images
Click the images to enlarge.
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: SMMC Cell Lysate
IHC(P): Human Mammary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,