Anti-VAMP2 Rabbit Monoclonal Antibody

SKU M02331-1
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number AAEG-22
Host Rabbit
Applications IP, IF, WB

Overview

Product Name Anti-VAMP2 Rabbit Monoclonal Antibody
SKU/Catalog Number M02331-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-VAMP2 Rabbit Monoclonal Antibodytested for Flow Cytometry, IP, IF, ICC, WB in Human, Mouse, Rat
Cite This Product Anti-VAMP2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02331-1)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human VAMP2
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:1000-1:5000
ICC/IF 1:50-1:200
IP 1:50
Flow Cytometry 1:50

Images And Assay Conditions

Western blot analysis of VAMP2 expression in mouse brain lysate (M02331-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAMP2 monoclonal antibody (Catalog # M02331-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAMP2

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P63027
Gene Name VAMP2
Protein Name Vesicle-associated membrane protein 2
Tissue Specificity Nervous system and skeletal muscle. .
Alternative Names Vesicle-associated membrane protein 2;VAMP-2;Synaptobrevin-2;VAMP2;SYB2;
Subcellular Localization Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Cell membrane . Neuronal synaptic vesicles. Colocalizes with PRKCZ and WDFY2 in intracellular vesicles (PubMed:17313651). .
Molecular Weight 12663 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Involved in the targeting and/or fusion of transport vesicles to their target membrane. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. .
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.

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Monoclonal antibody for VAMP-2/VAMP2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, ICC, WB. Reactive species: Human, Mouse, Rat VAMP-2/VAMP2 information: Molecular Weight: 12663 MW; Subcellular Localization: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Cell membrane . Neuronal synaptic vesicles. Colocalizes with PRKCZ and WDFY2 in intracellular vesicles (PubMed:17313651); Tissue Specificity: Nervous system and skeletal muscle.
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In stock
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M02331-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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