SKU M02331-1
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number AAEG-22
Host Rabbit
Applications IP, IF, WB

Overview

Product Name Anti-VAMP2 Rabbit Monoclonal Antibody
SKU/Catalog Number M02331-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-VAMP2 Rabbit Monoclonal Antibodytested for Flow Cytometry, IP, IF, ICC, WB in Human, Mouse, Rat
Cite This Product Anti-VAMP2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02331-1)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human VAMP2
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:1000-1:5000
ICC/IF 1:50-1:200
IP 1:50
Flow Cytometry 1:50

Images And Assay Conditions

/M/0/M02331 1 VAMP2 primary antibodies WB testing 1.jpg

Western blot analysis of VAMP2 expression in mouse brain lysate (M02331-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAMP2 monoclonal antibody (Catalog # M02331-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAMP2

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P63027
Gene Name VAMP2
Protein Name Vesicle-associated membrane protein 2
Tissue Specificity Nervous system and skeletal muscle. .
Alternative Names Vesicle-associated membrane protein 2;VAMP-2;Synaptobrevin-2;VAMP2;SYB2;
Subcellular Localization Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Cell membrane . Neuronal synaptic vesicles. Colocalizes with PRKCZ and WDFY2 in intracellular vesicles (PubMed:17313651). .
Molecular Weight 12663 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Involved in the targeting and/or fusion of transport vesicles to their target membrane. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. .
Research Areas Cancer, Cancer Metabolism, Cell Type Marker, Energy Transfer Pathways, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Neuron Marker, Neuroscience, Pathways And Processes, Protein Trafficking, Signal Transduction, Synapse Marker, Vesicle Transport

*You can search these to find other products in these research areas.

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Order Product (M02331-1)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

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