|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-VCAM-1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Vascular cell adhesion protein 1(VCAM1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-VCAM-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1873)|
|Replacement Item||This antibody may replace the following items: sc-13160|sc-13506|sc-1504|sc-1504-R|sc-1508|sc-15326|sc-18854|sc-18864|sc-20070|sc-31032|sc-31034|sc-31047|sc-31048|sc-33686|sc-374046|sc-53386|sc-53387|sc-59868|sc-71653|sc-73252|sc-73253|sc-8304|sc-8305 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse VCAM1(653-671aa YTIRQAQLQDAGIYECESK), different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Vascular cell adhesion protein 1|
|Molecular Weight||81317 MW|
|Protein Function||Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.|
|Tissue Specificity||Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. .|
|Sequence Similarities||Contains 7 Ig-like C2-type (immunoglobulin-like) domains.|
|Subcellular Localization||Isoform 1: Cell membrane; Single-pass type I membrane protein.|
|Alternative Names||Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;CD106;Vcam1;Vcam-1;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cell adhesion|cell adhesion molecules|vascular| signal transduction|endothelial| neuroscience|neurology process|neural signal transduction| stem cells|mesenchymal stem cells|surface molecules| cancer|invasion/microenvironment| cardiovascular|atherosclerosis|vascular inflammation|leukocyte recruitment| kits/ lysates/ other|elisa kits|adhesion molecules elisa kits|atherosclerotic proteins elisa kits||
Background for Vascular cell adhesion protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-VCAM-1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-VCAM-1 Antibody Images
Click the images to enlarge.
IHC(P): Rat Intestine Tissue
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole Cell lysates,
Lane 2: MDA-MB-231 whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: COLO-320 whole cell lysates,
Lane 5: A431 whole cell lysates,
Lane 6: A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- VCAM-1 antigen affinity purified polyclonal antibody (Catalog # PA1873) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCAM-1 at approximately 110KD. The expected band size for VCAM-1 is at 81KD.
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,