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SKU:PA1873
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-VCAM-1 Antibody
SKU/Catalog Number PA1873
Description Rabbit IgG polyclonal antibody for Vascular cell adhesion protein 1(VCAM1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-VCAM-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1873)
Replacement Item This antibody may replace the following items: sc-13160|sc-13506|sc-1504|sc-1504-R|sc-1508|sc-15326|sc-18854|sc-18864|sc-20070|sc-31032|sc-31034|sc-31047|sc-31048|sc-33686|sc-374046|sc-53386|sc-53387|sc-59868|sc-71653|sc-73252|sc-73253|sc-8304|sc-8305 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse VCAM1(653-671aa YTIRQAQLQDAGIYECESK), different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Vcam1
Protein Name Vascular cell adhesion protein 1
Molecular Weight 81317 MW
Protein Function Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Tissue Specificity Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. .
Sequence Similarities Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Subcellular Localization Isoform 1: Cell membrane; Single-pass type I membrane protein.
Uniprot ID P29533
Alternative Names Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;CD106;Vcam1;Vcam-1;
Research Areas |signal transduction|cytoskeleton / ecm|cell adhesion|cell adhesion molecules|vascular| signal transduction|endothelial| neuroscience|neurology process|neural signal transduction| stem cells|mesenchymal stem cells|surface molecules| cancer|invasion/microenvironment| cardiovascular|atherosclerosis|vascular inflammation|leukocyte recruitment| kits/ lysates/ other|elisa kits|adhesion molecules elisa kits|atherosclerotic proteins elisa kits|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Vascular cell adhesion protein 1

CD106(cluster of differentiation 106) also known as vascular cell adhesion molecule 1(VCAM-1),is a protein that in humans is encoded by the VCAM1 gene. In inflammatory conditions and in cardiac allografts undergoing rejection, VCAM1 is upregulated in endothelium of postcapillary venules. Arterial expression of VCAM1 is also found in experimental models of atherosclerosis in the rabbit. Cybulsky et al.(1991) mapped the VCAM1 gene to chromosome 1 by Southern analysis of somatic cell hybrids. Kumar et al.(1994) mapped the murine Vcam1 gene to chromosome 3 near Amy1.VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes,monocytes, eosinophils, andbasophils to vascularendothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development ofatherosclerosis and rheumatoid arthritis. CAM741 works similar to cotransin in that it represses the biosynthesis of VCAM1 cells by blocking the process of cotranslational translocation, which is dependent on the signal peptide ofVCAM1. Among the lung metastasis signature genes identified, several, includingVCAM1, were functionally validated.

Anti-VCAM-1 Antibody Images

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Anti-VCAM-1 Antibody
Anti-VCAM1 antibody, PA1873, IHC(P)
IHC(P): Rat Intestine Tissue
Anti-VCAM-1 Antibody
Figure 2. Western blot analysis of VCAM-1 using anti- VCAM-1 antibody (PA1873).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole Cell lysates,
Lane 2: MDA-MB-231 whole cell lysates,
Lane 3: HepG2 whole cell lysates,
Lane 4: COLO-320 whole cell lysates,
Lane 5: A431 whole cell lysates,
Lane 6: A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- VCAM-1 antigen affinity purified polyclonal antibody (Catalog # PA1873) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCAM-1 at approximately 110KD. The expected band size for VCAM-1 is at 81KD.
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Publications

Zhao Ly, Huang W, Yuan Qx, Cheng J, Huang Zc, Ouyang Lj, Zeng Fh. Food Chem. 2012 Sep 15;134(2):964-71. Doi: 10.1016/J.Foodchem.2012.03.001. Epub 2012 Mar 7. Hypolipidaemic Effects And Mechanisms Of The Main Component Of Opuntia Dillenii Haw. Poly...
Wang Yl, Wang Zj, Shen Hl, Yin M, Tang Kx. Eur J Pharm Sci. 2013 Nov 20;50(3-4):366-71. Doi: 10.1016/J.Ejps.2013.08.003. Epub 2013 Aug 15. Effects Of Artesunate And Ursolic Acid On Hyperlipidemia And Its Complications In Rabbit.
Liu M, Yu Y, Jiang H, Zhang L, Zhang Pp, Yu P, Jia Jg, Chen Rz, Zou Yz, Ge Jb. Acta Pharmacol Sin. 2013 Jun;34(6):830-6. Doi: 10.1038/Aps.2013.8. Epub 2013 Apr 8. Simvastatin Suppresses Vascular Inflammation And Atherosclerosis In Apoe(-/-) Mice B...
Wang Yq, Shen Y, Li F, Wang Ch, Zhang W. Cell Biochem Biophys. 2013;67(3):997-1004. Doi: 10.1007/S12013-013-9595-7. 2,3,4',5-Tetrahydroxystilbene-2-O-??-D-Glucoside Suppresses Expression Of Adhesion Molecules In Aortic Wall Of Dietary Atherosclerot...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: What do you suggest to use as negative control for this product? Keyword: controls, protocol
A: We use sample diluent instead of the primary antibody as the negative control.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to vcam 1 antibody, vcam-1 antibody, cd106 antibody, l1cam antibody, vcam1 antibody