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SKU:PA1780
Clonality:Polyclonal
Application:IHC-P, IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-VDAC/Porin Antibody
Description Rabbit IgG polyclonal antibody for Voltage-dependent anion-selective channel protein 1(VDAC1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.
Cite This Product Anti-VDAC/Porin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1780)
Replacement Item This antibody may replace the following items: sc-8828|sc-32064|sc-32063|sc-8829|sc-390996 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human VDAC(163-178aa RVTQSNFAVGYKTDEF), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name VDAC1
Protein Name Voltage-dependent anion-selective channel protein 1(VDAC-1/hVDAC1)
Molecular Weight 30773 MW
Protein Function Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. .
Tissue Specificity Heart, liver and skeletal muscle.
Sequence Similarities Belongs to the eukaryotic mitochondrial porin family.
Subcellular Localization Mitochondrion outer membrane . Cell membrane .
Uniprot ID P21796
Alternative Names Voltage-dependent anion-selective channel protein 1;VDAC-1;hVDAC1;Outer mitochondrial membrane protein porin 1;Plasmalemmal porin;Porin 31HL;Porin 31HM;VDAC1;VDAC;
Research Areas |tags & cell markers|subcellular markers|organelles|mitochondria| signal transduction|metabolism|mitochondrial| isotype/loading controls|vdac1 - mitochondrial preps| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|types of disease|cancer|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Voltage-dependent anion-selective channel protein 1(VDAC-1/hVDAC1)

The voltage-dependent anion channel(VDAC) of the outer mitochondrial membrane is a small, abundant outer membrane pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is though to form the major pathway for movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. At low transmembrane voltage, VDAC is open for anions such as phosphate, chloride, and adenine nucleotides. At higher transmembrane voltage, VDAC functions as a selective channel for cations and uncharged molecules. These features make VDAC likely to play a role in mitochondrial energy metabolism. Huizing et al. studied by Northern and Western blot analyses the human tissue distribution of mitochondrial transmembrane metabolite carriers. They found that VDAC1 mRNA has a ubiquitous distribution, with most pronounced expression in heart, liver, and skeletal muscle, whereas the VDAC2 isoform appears to be expressed only in the heart.

Anti-VDAC/Porin Antibody Images

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Anti-VDAC/Porin Antibody
Anti-VDAC/Porin antibody, PA1780, Western blotting
All lanes: Anti VDAC/Porin (PA1780) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 31KD
Observed bind size: 31KD
Anti-VDAC/Porin Antibody
Anti-VDAC/Porin antibody, PA1780, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Anti-VDAC/Porin Antibody
Anti-VDAC/Porin antibody, PA1780, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
Anti-VDAC/Porin Antibody
Anti-VDAC/Porin antibody, PA1780, ICC
ICC: NIH3T3 Cell
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Publications

L Wu, X Guo, SD Hartson, MA Davis, H He… - Molecular Nutrition & Food …, 2016 10.1002/mnfr.201600576 Lack of β, β‐carotene‐9', 10'‐oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to vdac antibody, porin antibody, vdac1 antibody