Product Info Summary
SKU: | M01168 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-VDAC1/Porin Rabbit Monoclonal Antibody
SKU/Catalog Number
M01168
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-VDAC1/Porin Rabbit Monoclonal Antibody catalog # M01168. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VDAC1/Porin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01168)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DBA-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human VDAC1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M01168 is reactive to VDAC1 in Human, Mouse, Rat
Applications
M01168 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
48 kDa
Calculated molecular weight
30.773kDa
Background of VDAC1
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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Western blot analysis of VDAC1 expression in (1) HepG2 cell lysate; (2) Jurkat cell lysate.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human stomach, using the Antibody at 1:2000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:2000 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse intestine, using the Antibody at 1:1000 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For VDAC1 (Source: Uniprot.org, NCBI)
Gene Name
VDAC1
Full Name
Voltage-dependent anion-selective channel protein 1
Weight
30.773kDa
Superfamily
eukaryotic mitochondrial porin family
Alternative Names
hVDAC1; MGC111064; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31HL; Porin 31HM; PORIN; PORIN-31-HL; VDAC; VDAC-1; voltage-dependent anion channel 1; voltage-dependent anion-selective channel protein 1 VDAC1 PORIN, VDAC-1 voltage dependent anion channel 1 voltage-dependent anion-selective channel protein 1|outer mitochondrial membrane protein porin 1|plasmalemmal porin|porin 31HL|porin 31HM|sperm binding protein 1a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on VDAC1, check out the VDAC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VDAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VDAC1/Porin Rabbit Monoclonal Antibody (M01168)
Hello CJ!
M01168 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Song H,Telugu BP,Thompson LP.Sexual dimorphism of mitochondrial function in the hypoxic guinea pig placenta.Biol Reprod.2019 Jan 1;100(1):208-216.doi:10.1093/biolre/ioy167.PMID:30085007;PMCID:PMC6335207.
Species: Pigs
M01168 usage in article: APP:WB, SAMPLE:MITOCHONDRIA, DILUTION: 1:2000
Wu L,Guo X,Wong SY,Lu P,Hartson SD,Medeiros DM,Wang W,Clarke SL,Lucas EA,Smith BJ,Chowanadisai W,Lin D. Deficiency of β-carotene oxygenase 2 induces mitochondrial fragmentation and activates the STING-IRF3 pathway in the mouse hypothalamus. J Nutr Biochem
Species: Mouse
M01168 usage in article: APP:WB, SAMPLE:HYPOTHALAMIC PROTEINS AND MITOCHONDRIAL FRACTIONS, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-VDAC1/Porin Rabbit Monoclonal Antibody
Question
Is there a BSA free version of anti-VDAC1/Porin Rabbit Monoclonal antibody M01168 available?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-VDAC1/Porin Rabbit Monoclonal antibody M01168 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-14
Question
Will anti-VDAC1/Porin Rabbit Monoclonal antibody M01168 work for IHC with cervix carcinoma?
R. Mitchell
Verified customer
Asked: 2020-02-14
Answer
According to the expression profile of cervix carcinoma, VDAC1 is highly expressed in cervix carcinoma. So, it is likely that anti-VDAC1/Porin Rabbit Monoclonal antibody M01168 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2020-02-14
Question
Will M01168 anti-VDAC1/Porin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
You can see on the product datasheet, M01168 anti-VDAC1/Porin Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-15
Question
We have been able to see staining in mouse skeletal muscle. Are there any suggestions? Is anti-VDAC1/Porin Rabbit Monoclonal antibody supposed to stain skeletal muscle positively?
K. Mitchell
Verified customer
Asked: 2019-12-24
Answer
From literature skeletal muscle does express VDAC1. From Uniprot.org, VDAC1 is expressed in biceps brachii, pituitary, thymus, lung skin, lymphocyte, skeletal muscle, b-cell, b-cell lymphoma, brain, cajal-retzius cell fetal brain cortex, cervix carcinoma, platelet, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VDAC1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 7685903
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Skeletal muscle, Pubmed ID: 1657034
Boster Scientific Support
Answered: 2019-12-24
Question
I see that the anti-VDAC1/Porin Rabbit Monoclonal antibody M01168 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-04
Question
Is a blocking peptide available for product anti-VDAC1/Porin Rabbit Monoclonal antibody (M01168)?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
We do provide the blocking peptide for product anti-VDAC1/Porin Rabbit Monoclonal antibody (M01168). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-02
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-VDAC1/Porin Rabbit Monoclonal antibody M01168. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-26
Question
My question regarding product M01168, anti-VDAC1/Porin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01168 anti-VDAC1/Porin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-22
Question
I am interested in using your anti-VDAC1/Porin Rabbit Monoclonal antibody for mitochondrial protein import studies. Has this antibody been tested with western blotting on hepg2 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
We appreciate your inquiry. This M01168 anti-VDAC1/Porin Rabbit Monoclonal antibody is validated on hepg2 cell lysate, jurkat cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-18
Question
Our lab used your anti-VDAC1/Porin Rabbit Monoclonal antibody for IHC on lymphocyte in the past. I am using human, and We want to use the antibody for WB next. I was wanting to use examining lymphocyte as well as biceps brachii in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
I have checked the website and datasheets of our anti-VDAC1/Porin Rabbit Monoclonal antibody and it seems that M01168 has been validated on human in both IHC and WB. Thus M01168 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-31
Question
My lab would like to test anti-VDAC1/Porin Rabbit Monoclonal antibody M01168 on human cervix carcinoma for research purposes, then I may be interested in using anti-VDAC1/Porin Rabbit Monoclonal antibody M01168 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
The products we sell, including anti-VDAC1/Porin Rabbit Monoclonal antibody M01168, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-01
Question
I was wanting to use your anti-VDAC1/Porin Rabbit Monoclonal antibody for IHC for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
As indicated on the product datasheet, M01168 anti-VDAC1/Porin Rabbit Monoclonal antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-15
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-VDAC1/Porin Rabbit Monoclonal antibody M01168. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-19
Question
My boss were satisfied with the WB result of your anti-VDAC1/Porin Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma mitochondrion outer membrane using this antibody. Is that expected? Could you tell me where is VDAC1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
From what I have seen in literature, cervix carcinoma does express VDAC1. Generally VDAC1 expresses in mitochondrion outer membrane. Regarding which tissues have VDAC1 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 7685903
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Skeletal muscle, Pubmed ID: 1657034
Boster Scientific Support
Answered: 2018-05-01
Question
Is this M01168 anti-VDAC1/Porin Rabbit Monoclonal antibody reactive to the isotypes of VDAC1?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
The immunogen of M01168 anti-VDAC1/Porin Rabbit Monoclonal antibody is A synthesized peptide derived from human VDAC1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-14
Question
We are currently using anti-VDAC1/Porin Rabbit Monoclonal antibody M01168 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
J. Krishna
Verified customer
Asked: 2016-05-19
Answer
The anti-VDAC1/Porin Rabbit Monoclonal antibody (M01168) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-19