Product Info Summary
SKU: | A02632-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-VE-Cadherin Cdh5-Antibody Picoband™
View all VE-Cadherin Antibodies
SKU/Catalog Number
A02632-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VE-Cadherin Cdh5-Antibody Picoband™ catalog # A02632-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VE-Cadherin Cdh5-Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02632-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse VE Cadherin recombinant protein (Position: D46-E285).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02632-2 is reactive to CDH5 in Mouse, Rat
Applications
A02632-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
120 kDa
Calculated molecular weight
87.528kDa
Background of VE-Cadherin
CDH5 (Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VE Cadherin antigen affinity purified polyclonal antibody (Catalog # A02632-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VE Cadherin at approximately 120 kDa. The expected band size for VE Cadherin is at 88 kDa.
Click image to see more details
Figure 2. IHC analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-2).
VE Cadherin was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VE Cadherin Antibody (A02632-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of VE Cadherin using anti-VE Cadherin antibody (A02632-2).
VE Cadherin was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VE Cadherin Antibody (A02632-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. Sandwich ELISA - Recombinant mouse VE Cadherin/Cdh5 protein standard curve.
Use in combination with reagents from Mouse VE Cadherin/Cdh5 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1318).
Protein Target Info & Infographic
Gene/Protein Information For CDH5 (Source: Uniprot.org, NCBI)
Gene Name
CDH5
Full Name
Cadherin-5
Weight
87.528kDa
Alternative Names
7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin CDH5 7B4, CD144 cadherin 5 cadherin-5|7B4 antigen|VE-cadherin|cadherin 5, type 2 (vascular endothelium)|cadherin 5, type 2, VE-cadherin (vascular epithelium)|cd144 antigen|endothelial-specific cadherin|vascular endothelial cadherin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH5, check out the CDH5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-VE-Cadherin Cdh5-Antibody Picoband™ (A02632-2)
Hello CJ!
A02632-2 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Metformin Promotes the Survival of Random-Pattern Skin Flaps by Inducing Autophagy via the AMPK-mTOR-TFEB signaling pathway
Butylphthalide Inhibits Autophagy and Promotes Multiterritory Perforator Flap Survival
Salvianolic Acid B Promotes the Survival of Random-Pattern Skin Flaps in Rats by Inducing Autophagy
Targeting TFE3 Protects Against Lysosomal Malfunction-Induced Pyroptosis in Random Skin Flaps via ROS Elimination
Therapeutic potential of pravastatin for random skin flaps necrosis: involvement of promoting angiogenesis and inhibiting apoptosis and oxidative stress
FGF21 augments autophagy in random-pattern skin flaps via AMPK signaling pathways and improves tissue survival
Zhou F,Zhang X,Jiang L,Li S,Chen Y and Wu J(2021) Pseudoginsenoside F11 Enhances the Viability of Random-Pattern Skin Flaps by Promoting TFEB Nuclear Translocation Through AMPK-mTOR Signal Pathway.Front.Pharmacol.12:667524.doi:10.3389/fphar.2021.667524
Species: Rat
A02632-2 usage in article: APP:WB, SAMPLE:FLAP TISSUE, DILUTION:NA
Zhang C,Shao Z,Chen Z,Lin C,Hu S,Lou Z,Li J,Zheng X,Lin N,Gao W.Hydroxysafflor yellow A promotes multiterritory perforating flap survival: an experimental study.Am J Transl Res.2020 Aug 15;12(8):4781-4794.PMID:32913550;PMCID:PMC7476167.
Species: Rat
A02632-2 usage in article: APP:WB, SAMPLE:SKIN TISSUE, DILUTION:1:1000
Li J,Chen H,Lou J,Bao G,Wu C,Lou Z,Wang X,Ding J,Li Z,Xiao J,Xu H,Gao W,Zhou K.Exenatide improves random-pattern skin flap survival via TFE3 mediated autophagy augment.J Cell Physiol.2020 Oct 12.doi:10.1002/jcp.30102.Epub ahead of print.PMID:33044023.
Species: Mouse
A02632-2 usage in article: APP:WB, SAMPLE:FLAP TISSUE, DILUTION:1:1000
Metformin Promotes the Survival of Random-Pattern Skin Flaps by Inducing Autophagy via the AMPK-mTOR-TFEB signaling pathway Hongqiang Wu,1,2,3,* Jian Ding,1,2,3,* Shihen Li,1,2,3 Jinti Lin,1,2,3 Renhao Jiang,1,2,3 Chen Lin,1,2,3 Li Dai,1,2,3 Chenglong X
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