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SKU:PB9071
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-VEGF Picoband™ Antibody
SKU/Catalog Number PB9071
Description Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB, IHC-P in Human;Rat.
Cite This Product Anti-VEGF Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9071)
Replacement Item This antibody may replace the following items: sc-152|sc-152-G|sc-365578|sc-507|sc-53462|sc-53463|sc-57496|sc-65617|sc-7269 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human VEGF recombinant protein (Position: A27-R191). Human VEGF shares 78% amino acid (aa) sequence identity with both mouse and rat VEGF.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name VEGFA
Protein Name Vascular endothelial growth factor A
Molecular Weight 27042 MW
Protein Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. .
Tissue Specificity Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland. .
Sequence Similarities Belongs to the PDGF/VEGF growth factor family.
Subcellular Localization Secreted . VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
Uniprot ID P15692
Alternative Names Vascular endothelial growth factor A;VEGF-A;Vascular permeability factor;VPF;VEGFA;VEGF;
Research Areas |cardiovascular|angiogenesis|growth factors|vegf| signal transduction|growth factors/hormones| cancer|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes|types of disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Vascular endothelial growth factor A

VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. And this gene also can regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.

Anti-VEGF Picoband™ Antibody Images

Click the images to enlarge.

Anti-VEGF Picoband™ Antibody
Anti-VEGF Picoband antibody, PB9071-1.JPG
IHC(P): Human Lung Cancer Tissue
Anti-VEGF Picoband™ Antibody
Anti-VEGF Picoband antibody, PB9071-2.JPG
IHC(P): Human Placenta Tissue
Anti-VEGF Picoband™ Antibody
Anti-VEGF Picoband antibody, PB9071-3.jpg
All lanes: Anti-VEGF(PB9071) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
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Publications

KD Zhang, LR Tong, SM Wang, RY Peng, HD Huang… - Chinese Medical Journal, 2017 Apoptosis of Lewis Lung Carcinoma Cells Induced by Microwave via p53 and Proapoptotic Proteins In vivo
Sun J, Wang S, Wei Yh. J Ethnopharmacol. 2011 Oct 11;137(3):1498-503. Doi: 10.1016/J.Jep.2011.08.026. Epub 2011 Aug 22. Reproductive Toxicity Of Rhizoma Sparganii (Sparganium Stoloniferum Buch.-Ham.) In Mice: Mechanisms Of Anti-Angiogenesis And An...
Zhu L, Wei W, Zheng Yq, Jia Xy. Inflamm Res. 2005 May;54(5):211-20. Effects And Mechanisms Of Total Glucosides Of Paeony On Joint Damage In Rat Collagen-Induced Arthritis.
Liu S, Liu T, Chen J, Maitz M, Chen C, Huang N. J Biomed Mater Res A. 2013 Apr;101(4):1144-57. Doi: 10.1002/Jbm.A.34392. Epub 2012 Oct 9. Influence Of A Layer-By-Layer-Assembled Multilayer Of Anti-Cd34 Antibody, Vascular Endothelial Growth Factor,...
Su B, Xiang B, Wang L, Cao L, Xiao L, Li X, Li X, Wu M, Li G. J Cell Biochem. 2010 Jan 1;109(1):173-83. Doi: 10.1002/Jcb.22395. Profiling And Comparing Transcription Factors Activated In Non-Metastatic And Metastatic Nasopharyngeal Carcinoma Cells.
Huang S, Yang N, Liu Y, Gao J, Huang T, Hu L, Zhao J, Li Y, Li C, Zhang X. Int J Mol Med. 2012 Dec;30(6):1410-6. Doi: 10.3892/Ijmm.2012.1147. Epub 2012 Oct 1. Grape Seed Proanthocyanidins Inhibit Colon Cancer-Induced Angiogenesis Throu...
Liu Mw, Su Mx, Zhang W, Wang Yh, Qin Lf, Liu X, Tian Ml, Qian Cy. Mol Med Rep. 2015 May;11(5):3308-16. Doi: 10.3892/Mmr.2015.3146. Epub 2015 Jan 7. Effect Of Melilotus Suaveolens Extract On Pulmonary Microvascular Permeability By Downregulating Va...
Zhang B, Zhang Y, Yao G, Gao J, Yang B, Zhao Y, Rao Z, Gao J. Clinics (Sao Paulo). 2012 Aug;67(8):901-6. M2-Polarized Macrophages Promote Metastatic Behavior Of Lewis Lung Carcinoma Cells By Inducing Vascular Endothelial Growth Factor-C Expression.
Xuebing P, Tinchiu L, Enlan X, Jing L, Xiaowu H. Eur J Obstet Gynecol Reprod Biol. 2011 Nov;159(1):198-203. Doi: 10.1016/J.Ejogrb.2011.06.036. Epub 2011 Jul 2. Is Endometrial Polyp Formation Associated With Increased Expression Of Vascular Endothe...
Jin M, Zhang Y, Pan L, Dou R, Nussenblatt Rb, Wei L. J Transl Med. 2012 Jun 7;10:118. Doi: 10.1186/1479-5876-10-118. The Chinese Medicine Formula Hb01 Reduces Choroidal Neovascularization By Regulating The Expression Of Vascular Endothelial Growth...
Li X, Wang H, Lu X, Di B. Exp Ther Med. 2010 Nov;1(6):947-953. Epub 2010 Sep 29. Silencing Stat3 With Short Hairpin Rna Enhances Radiosensitivity Of Human Laryngeal Squamous Cell Carcinoma Xenografts In Vivo.
Zhang S, Yu H, Zhang L. Oncol Lett. 2010 May;1(3):453-456. Epub 2010 May 1. Role Of Vascular Endothelial Growth Factor Receptor-3/Flt-4 In Early-Stage Cervical Cancer.
Tang C, Wu X, Ye L, Xie X, Wang G. Biocell. 2012 Dec;36(3):97-103. Effects Of Rotational Culture On Morphology, Nitric Oxide Production And Cell Cycle Of Endothelial Cells.
Zhao W, Shen H, Yuan F, Li G, Sun Y, Shi Z, Zhang Y, Wang Z. Eur J Histochem. 2009 Jan-Mar;53(1):53-60. Induction Stage-Dependent Expression Of Vascular Endothelial Growth Factor And Aquaporin-1 In Diethylstilbestrol-Treated Rat Pituitary.
Liang Ql, Wang Br, Chen Gq, Li Gh, Xu Yy. Med Oncol. 2010 Dec;27(4):1164-70. Doi: 10.1007/S12032-009-9354-1. Epub 2009 Nov 12. Clinical Significance Of Vascular Endothelial Growth Factor And Connexin43 For Predicting Pancreatic Cancer Clinicopatho...
Zhan Cy, Yuan Tm, Sun Y, Yu Hm. J Matern Fetal Neonatal Med. 2011 Feb;24(2):213-22. Doi: 10.3109/14767051003758895. Epub 2010 May 17. Early Gestational Intrauterine Infection Induces Postnatal Lung Inflammation And Arrests Lung Development In A Ra...
Zhang Y, Zhang H, Chen J, Zhao H, Zeng X, Zhang H, Qing C. Invest New Drugs. 2012 Feb;30(1):8-16. Doi: 10.1007/S10637-010-9501-8. Epub 2010 Aug 14. Antitumor And Antiangiogenic Effects Of Ga-13315, A Gibberellin Derivative.
Zhang L, Ge W, Hu K, Zhang Y, Li C, Xu X, He D, Zhao Z, Zhang J, Jie F, Chen Y, Zheng Y. Mol Biol Rep. 2012 Jan;39(1):89-95. Doi: 10.1007/S11033-011-0713-6. Epub 2011 May 13. Endostar Down-Regulates Hif-1 And Vegf Expression And Enhances The Radio...
Jiang X, Ge H, Zhou C, Chai X, Ren Qs. Lasers Med Sci. 2012 May;27(3):599-606. Doi: 10.1007/S10103-011-0996-9. Epub 2011 Oct 1. The Role Of Vascular Endothelial Growth Factor In Fractional Laser Resurfacing With The Carbon Dioxide Laser.
Guo Q, Jin J, Yuan Jx, Zeifman A, Chen J, Shen B, Huang J. Biochem Biophys Res Commun. 2011 Oct 7;413(4):630-6. Doi: 10.1016/J.Bbrc.2011.09.015. Epub 2011 Sep 12. Vegf, Bcl-2 And Bad Regulated By Angiopoietin-1 In Oleic Acid Induced Acute Lung Inj...
Zhang T, Liu M, Wang C, Lin C, Sun Y, Jin D. Anticancer Res. 2011 Nov;31(11):3859-63. Down-Regulation Of Mir-206 Promotes Proliferation And Invasion Of Laryngeal Cancer By Regulating Vegf Expression.
Zhang W, Yan H. Exp Eye Res. 2012 Dec;105:1-8. Doi: 10.1016/J.Exer.2012.09.014. Epub 2012 Oct 8. Simvastatin Increases Circulating Endothelial Progenitor Cells And Reduces The Formation And Progression Of Diabetic Retinopathy In Rats.
Jia Y, Wu J, Chen L, Liao G, Yang J, Tang L, Li C. Am J Med Sci. 2013 Oct;346(4):303-7. Doi: 10.1097/Maj.0B013E318272087A. Focused Ultrasound Therapy For Vulvar Intraepithelial Neoplasia In A Mice Model.
Ling S, Deng D, Mo Y, Zhang X, Guan X, Wei Q. Cell Biochem Biophys. 2014 Sep;70(1):629-33. Doi: 10.1007/S12013-014-9966-8. Correlations Between Ct Perfusion Parameters And Vascular Endothelial Growth Factor Expression And Microvessel Density In Im...
Liu Y, Liu Y, Jiang H, Xu L, Cheng Y, Wang Pg, Wang F. Carbohydr Polym. 2014 Jun 15;106:42-8. Doi: 10.1016/J.Carbpol.2014.01.088. Epub 2014 Feb 4. Preparation, Antiangiogenic And Antitumoral Activities Of The Chemically Sulfated Glucan From Phelli...
Hou C, Kong J, Liang Y, Huang H, Wen H, Zheng X, Wu L, Chen Y. Cell Mol Immunol. 2015 Jul;12(4):409-23. Doi: 10.1038/Cmi.2014.60. Epub 2014 Aug 25. Hmgb1 Contributes To Allergen-Induced Airway Remodeling In A Murine Model Of Chronic Asthma By Modu...
Wang Z, Lv J, Zhang T. Oncol Rep. 2015 May;33(5):2468-76. Doi: 10.3892/Or.2015.3853. Epub 2015 Mar 16. Combination Of Il-24 And Cisplatin Inhibits Angiogenesis And Lymphangiogenesis Of Cervical Cancer Xenografts In A Nude Mouse Model By Inhibiting...
Hao Jh, Yu M, Li Hk, Shi Yr, Li Q, Hao Xs. World J Gastroenterol. 2006 Feb 21;12(7):1140-3. Inhibitory Effect Of Antisense Vascular Endothelial Growth Factor Rna On The Profile Of Hepatocellular Carcinoma Cell Line In Vitro And In Vivo.
Ye Y, Huang C, Jiang L, Shen X, Zhu S, Rao Y, Wang J, Zhang Q. Biol Pharm Bull. 2012;35(3):355-61. Huanglian-Jie-Du-Tang Extract Protects Against Chronic Brain Injury After Focal Cerebral Ischemia Via Hypoxia-Inducible-Factor-1??-Regulated Vascular...
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...
Zhao Y, Zong Zh, Xu Hm. Gynecol Oncol. 2010 Mar;116(3):563-71. Doi: 10.1016/J.Ygyno.2009.11.015. Rhoc Expression Level Is Correlated With The Clinicopathological Characteristics Of Ovarian Cancer And The Expression Levels Of Rock-I, Vegf, And Mmp9.
Liu Y, Zhou Y, Zhang Xs, Shen Bz. Oncol Lett. 2011 Nov;2(6):1171-1175. Epub 2011 Aug 17. Expression Of Vegf And Mmp-9 And Mri Imaging Changes In Cerebral Glioma.
Zhang Lh, Jia Yl, Lin Xx, Zhang Hq, Dong Xw, Zhao Jm, Shen J, Shen Hj, Li Ff, Yan Xf, Li W, Zhao Yq, Xie Qm. Biochim Biophys Acta. 2013 Aug;1830(8):4148-59. Doi: 10.1016/J.Bbagen.2013.04.008. Epub 2013 Apr 11. Ad-1, A Novel Ginsenoside Derivative,...
Zhan X, Jia L, Niu Y, Qi H, Chen X, Zhang Q, Zhang J, Wang Y, Dong L, Wang C. Biomaterials. 2014 Dec;35(38):10046-57. Doi: 10.1016/J.Biomaterials.2014.09.007. Epub 2014 Sep 22. Targeted Depletion Of Tumour-Associated Macrophages By An Alendronate-...
Zhou J, Wang L, Xu X, Tu Y, Qin S, Yin Y. Oncol Lett. 2012 Nov;4(5):976-980. Epub 2012 Aug 8. Antitumor Activity Of Endostar Combined With Radiation Against Human Nasopharyngeal Carcinoma In Mouse Xenograft Models.
Kong J, Kong J, Pan B, Ke S, Dong S, Li X, Zhou A, Zheng L, Sun Wb. Plos One. 2012;7(5):E37266. Doi: 10.1371/Journal.Pone.0037266. Epub 2012 May 15. Insufficient Radiofrequency Ablation Promotes Angiogenesis Of Residual Hepatocellular Carcinoma Vi...
Duan J, Zhang Y, Han S, Chen Y, Li B, Liao M, Chen W, Deng X, Zhao J, Huang B. Int J Pharm. 2010 Nov 15;400(1-2):211-20. Doi: 10.1016/J.Ijpharm.2010.08.033. Epub 2010 Sep 8. Synthesis And In Vitro/In Vivo Anti-Cancer Evaluation Of Curcumin-Loaded ...
Xie Y, Chen S, Wang Ch, Tang Cw. Cancer Biol Ther. 2011 Jul 1;12(1):86-92. Doi: 10.4161/Cbt.12.1.15730. Epub 2011 Jul 1. Som230 Combined With Celecoxib Prolongs The Survival In Nude Mice With Hepg-2 Xenografts.
Li Jk, Yu L, Shen Y, Zhou Ls, Wang Yc, Zhang Jh. World J Gastroenterol. 2008 Apr 21;14(15):2308-13. Inhibition Of Cxcr4 Activity With Amd3100 Decreases Invasion Of Human Colorectal Cancer Cells In Vitro.
Zhao X, Sun X, Li Xl. Asian Pac J Cancer Prev. 2012;13(6):2873-7. Expression And Clinical Significance Of Stat3, P-Stat3, And Vegf-C In Small Cell Lung Cancer.
Ran J, Ma J, Liu Y, Tan R, Liu H, Lao G. J Diabetes Res. 2014;2014:287536. Doi: 10.1155/2014/287536. Epub 2014 Mar 13. Low Protein Diet Inhibits Uric Acid Synthesis And Attenuates Renal Damage In Streptozotocin-Induced Diabetic Rats.
Lu Ql, Liu J, Zhu Xl, Xu Wj. J Huazhong Univ Sci Technolog Med Sci. 2014 Jun;34(3):359-62. Doi: 10.1007/S11596-014-1283-3. Epub 2014 Jun 18. Expression Of Nerve Growth Factor And Hypoxia Inducible Factor-1?? And Its Correlation With Angiogenesis In...
Yu Zy, Bai Yn, Luo Lx, Wu H, Zeng Y. Mol Med Rep. 2013 Jul;8(1):287-93. Doi: 10.3892/Mmr.2013.1493. Epub 2013 May 28. Expression Of Microrna-150 Targeting Vascular Endothelial Growth Factor-A Is Downregulated Under Hypoxia During Liver Regeneration.
Zhang L, Wu X, Luo C, Chen X, Yang L, Tao J, Shi J. Mol Med Rep. 2013 Jul;8(1):272-6. Doi: 10.3892/Mmr.2013.1458. Epub 2013 May 8. The 786-0 Renal Cancer Cell-Derived Exosomes Promote Angiogenesis By Downregulating The Expression Of Hepatocyte Cel...
Hu J, Zhou Z, Shi S, Zhu X, Wang X, Zhang W, Hu S, Qian H, Xu W. Oncol Lett. 2013 Jan;5(1):179-184. Epub 2012 Oct 30. Mesenchymal Stem-Like Cells Isolated From Human Esophageal Carcinoma And Adjacent Non-Cancerous Tissues.
Duan F, Lin M, Li C, Ding X, Qian G, Zhang H, Ge S, Fan X, Li J. Cancer Biol Ther. 2014 May;15(5):544-59. Doi: 10.4161/Cbt.28157. Epub 2014 Mar 11. Effects Of Inhibition Of Hedgehog Signaling On Cell Growth And Migration Of Uveal Melanoma Cells.
Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1
Chen P, Wang L, Li N, Liu Q, Ni J. Metallomics. 2013 May;5(5):541-50. Doi: 10.1039/C3Mt00002H. Epub 2013 Apr 16. Comparative Proteomics Analysis Of Sodium Selenite-Induced Apoptosis In Human Prostate Cancer Cells.
Zhao M, Shi X, Liang J, Miao Y, Xie W, Zhang Y, Li X. Exp Eye Res. 2011 Dec;93(6):921-6. Doi: 10.1016/J.Exer.2011.10.013. Epub 2011 Nov 4. Expression Of Pro- And Anti-Angiogenic Isoforms Of Vegf In The Mouse Model Of Oxygen-Induced Retinopathy.
Wang J, Zhao W, Guo Y, Zhang B, Xie Q, Xiang D, Gao J, Wang B, Chen Z. Oncology. 2009;76(6):420-9. Doi: 10.1159/000216837. Epub 2009 May 5. The Expression Of Rna-Binding Protein Hur In Non-Small Cell Lung Cancer Correlates With Vascular Endothelia...
Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.
Ge Y, Ding Y, Zhang J, Li Z, Li Z. Int J Clin Exp Med. 2014 Oct 15;7(10):3578-82. Ecollection 2014. Effect Of Angiogenesis Inhibitor Su6668 In Combination With 5-Fu On Liver Metastasis From Transplantation Tumors Of Human Colorectal Cancer In Nude...
Wu H, Deng J, Yu S, Wang X, Chen Y. J Huazhong Univ Sci Technolog Med Sci. 2010 Feb;30(1):108-12. Doi: 10.1007/S11596-010-0120-6. Epub 2010 Feb 14. The Inhibitory Effects Of Rh-Endostatin (Yh-16) In Combination With Radiotherapy On Lung Adenocarci...
Yang W, Zhang Y, Yu J, Li S. Eur J Surg Oncol. 2010 May;36(5):501-6. Doi: 10.1016/J.Ejso.2010.01.007. Epub 2010 Feb 23. The Low Expression Of Cd80 Correlated With The Vascular Endothelial Growth Factor In Esophageal Cancer Tissue.
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to vegfa antibody, vegf-a antibody