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SKU:MA1040
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:KDR-1
Application:IHC
Price: $200.00
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Overview

Product Name Anti-VEGFR2/KDR Antibody
SKU/Catalog Number MA1040
Description Mouse IgG monoclonal antibody for VEGFR2, kinase insert domain receptor (a type III receptor tyrosine kinase) (KDR) detection. Tested with IHC-F in Human. No cross reactivity with other proteins.
Cite This Product Anti-VEGFR2/KDR Antibody KDR-1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1040)
Replacement Item This antibody may replace the following items: sc-101819|sc-101820|sc-101821|sc-16628|sc-16628-R|sc-16629-R|sc-19530|sc-393163|sc-393179|sc-48161|sc-504|sc-505|sc-6251 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application IHC

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant human extracellular domain of VEGFR-2(KDR).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number KDR-1
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Kdr
Protein Name Vascular endothelial growth factor receptor 2
Molecular Weight 150394 MW
Protein Function Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol-1,4,5- trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA- mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC (By similarity). .
Tissue Specificity Expressed in the post-pubertal mammary glands. .
Sequence Similarities Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Subcellular Localization Cell membrane ; Single-pass type I membrane protein . Cytoplasm . Nucleus . Cytoplasmic vesicle . Early endosome . Cell junction . Endoplasmic reticulum . Detected on caveolae-enriched lipid rafts at the cell surface. Is recycled from the plasma membrane to endosomes and back again. Phosphorylation triggered by VEGFA binding promotes internalization and subsequent degradation. VEGFA binding triggers internalization and translocation to the nucleus. Localized with RAP1A at cell-cell junctions. Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (By similarity). .
Uniprot ID O08775
Alternative Names Vascular endothelial growth factor receptor 2;VEGFR-2;2.7.10.1;Fetal liver kinase 1;FLK-1;Protein-tyrosine kinase receptor flk-1;CD309;Kdr;Flk1;
Research Areas |cardiovascular|angiogenesis|growth factors|vegf|vegf receptors| microbiology|interspecies interaction|host virus interaction| signal transduction|protein phosphorylation|tyrosine kinases|receptor tyrosine kinases|growth factors/hormones| stem cells|hematopoietic progenitors|surface molecules| cancer|invasion/microenvironment|angiogenic growth factors|oncoproteins/suppressors|oncoproteins|growth factor receptors|endothelial progenitors|endothelial markers|cancer metabolism|response to hypoxia| cardiovascular|cardiovascular markers|cell markers|endothelial cells| metabolism|pathways and processes|metabolism processes|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Vascular endothelial growth factor receptor 2

VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. The importance of VEGF and its receptor system in tumor growth and intervention in this system may provide promising approaches to cancer therapy. VEGF receptor 2 is a member of a receptor tyrosine kinase family. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues.
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Publications

DEXIANG ZHOU, SHENGQUAN ZHAN, DONG ZHOU, PENG WANG, GUANGZHONG CHEN, KUN QIN and XIAOFENG LIN EXPERIMENTAL AND THERAPEUTIC MEDICINE 13: 483-488, 2017 Characterizing nanoscale changes in the activity of VEGFR-2 on glioma microvascular endothelial cell membranes using atomic force microscopy
Hao Yanga, Juan Wanga, Jin-hong Fana, Ya-qi Zhanga, Jun-xian Zhaoa, Xiao-jun Daia, Qi Liua, Yan-jun Shena, Chang Liua, Wei-dong Suna, Yun Suna Toxicology and Applied Pharmacology Volume 315, 15 January 2017, Pages 90–101 Ilexgenin A exerts anti-inflammation and anti-angiogenesis effects through inhibition of STAT3 and PI3K pathways and exhibits synergistic effects with Sorafenib on hepatoma growth
Zhou D, Zhan S, Zhou D, Li Z, Lin X, Tang K, Shu H, Chen G, Zeng S, Cai Y, Jiang X. Cell Mol Neurobiol. 2011 Jul;31(5):687-94. Doi: 10.1007/S10571-011-9665-6. Epub 2011 Mar 9. A Study Of The Distribution And Density Of The Vegfr-2 Receptor On Glio...
Li Ql, Huang N, Chen C, Chen Jl, Xiong Kq, Chen Jy, You Tx, Jin J, Liang X. J Biomed Mater Res A. 2010 Sep 15;94(4):1283-93. Doi: 10.1002/Jbm.A.32812. Oriented Immobilization Of Anti-Cd34 Antibody On Titanium Surface For Self-Endothelialization In...
Sun Y, Chen S, Song G, Ren L, Wei L, Liu N, Zhang D, Lv X. Int J Mol Med. 2012 Sep;30(3):622-8. Doi: 10.3892/Ijmm.2012.1032. Epub 2012 Jun 14. Effect Of Visfatin On The Function Of Endothelial Progenitor Cells In High-Fat-Fed Obese Rat...
Lin J, Li G, Den X, Xu C, Liu S, Gao Y, Liu H, Zhang J, Li X, Liang S. Brain Res Bull. 2010 Oct 30;83(5):284-91. Doi: 10.1016/J.Brainresbull.2010.08.002. Epub 2010 Aug 10. Vegf And Its Receptor-2 Involved In Neuropathic Pain Transmission Mediated ...
Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1
Ding G, Chen X, Zhu J, Feng Z. Cytotechnology. 2014 May;66(3):395-411. Doi: 10.1007/S10616-013-9587-X. Epub 2013 Jul 24. A Murine-Human Chimeric Igg Antibody Against Vascular Endothelial Growth Factor Receptor 2 Inhibits Angiogenesis In Vitro.
Wang L, Liu X, Wang H, Wang S. Oncol Lett. 2013 Jul;6(1):175-180. Epub 2013 May 15. Correlation Of The Expression Of Vascular Endothelial Growth Factor And Its Receptors With Microvessel Density In Ovarian Cancer.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to vegfr2 antibody, flk1 antibody, flk-1 antibody, kdr antibody, vegfr 2 antibody, vegfr antibody, vegfr-2 antibody