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Product Info Summary
|Reactive Species:||Bovine, Chicken, Equine, Human, Mouse, Pig, Rat|
|Application:||IF, IHC, ICC, WB|
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Boster Bio Anti-Vimentin Antibody catalog # M00235-5. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Stable at 4 ° C for at least one year
Cite This Product
Anti-Vimentin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00235-5)
Supplied as an aliquot of IgY preparation plus 5mM NaN3
Full length recombinant human vimentin protein , PROT-r-Vim, expressed in and purified from E. coli.
M00235-5 is reactive to VIM in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
M00235-5 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
IF/ICC and IHC: 1:5000.
Validation Images & Assay Conditions
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Western blot analysis of tissue and cell lysates using chicken pAb to Vimentin, M00235-5, dilution 1:5,000 in red.  protein standard (red),  rat whole brain lysate,  HeLa,  SH-SY5Y,  HEK293, and  NIH-3T3 cell lysates. M00235-5 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes, and ~70kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
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Immunofluorescent analysis of HeLa cell culture stained with chicken pAb to vimentin, M00235-5, dilution 1:10,000 in green, and costained with Boster mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Gene/Protein Information For VIM (Source: Uniprot.Org, NCBI)
intermediate filament family
FLJ36605; VIM; Vimentin VIM vimentin vimentin|epididymis secretory sperm binding protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on VIM, check out the VIM Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for VIM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M00235-5 has been cited in 31 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu R,Xu B,Yu S,Zhang J,Sun H,Liu C,Lu F,Pan Q,Zhang X.Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts and Mycoplasma synoviae.Front Microbiol.2020 Apr 3;11:576.doi:10.3389/fmicb.2020.00576.PMID:323
M00235-5 usage in article: APP:IF, SAMPLE:CSF, DILUTION:NA
Zhang XW,Li QH,Xu ZD,Dou JJ.STAT1-induced regulation of lncRNA ZFPM2-AS1 predicts poor prognosis and contributes to hepatocellular carcinoma progression via the miR-653/GOLM1 axis.Cell Death Dis.2021 Jan 4;12(1):31.doi:10.1038/s41419-020-03300-4.PMID:3341
M00235-5 usage in article: APP:WB, SAMPLE:HCC CELL, DILUTION:1:800
Jue-Ying Chen,Ji-Wei Qiu,Jun-Lin Mo,et al.Effects of Yishenbupi (tonifying-kidney and invigorating-spleen) prescription on the expression of renal fibrosis-associated proteins in unilateral ureteral occlusion rats.TMR Integrative Medicine 2020,4:e20012.
M00235-5 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000
Jiang SB,Lu YS,Liu T,Li LM,Wang HX,Wu Y,Gao XH,Chen HD. UVA influenced the SIRT1-miR-27a-5p-SMAD2-MMP1/COL1/BCL2 axis in human skin primary fibroblasts.J Cell Mol Med.2020 Sep;24(17):10027-10041.doi: 10.1111/jcmm.15610.Epub 2020 Aug 13.PMID:32790210;PMCID
M00235-5 usage in article: APP:IF, SAMPLE:SKIN PRIMARY FIBROBLASTS, DILUTION:1:100
The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation
Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments
Autophagy Is Indispensable for the Self-Renewal and Quiescence of Ovarian Cancer Spheroid Cells with Stem Cell-Like Properties
Expression of E-cadherin and vimentin in oral squamous cell carcinoma
%u03B22-microglobulin induces epithelial-mesenchymal transition in human renal proximal tubule epithelial cells in vitro
Distinct Roles For ROCK1 and ROCK2 in the Regulation of Oxldl-Mediated Endothelial Dysfunction
MTDH promotes glioma invasion through regulating miR-130b-ceRNAs
Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human %u2026
Mesenchymal Stem Cells Promote Tumor Progression via Inducing Stroma Remodeling on Rabbit VX2 Bladder Tumor Model
Changes in hippocampal ultrastructure and vimentin expression in rhesus monkeys following selective deep hypothermia and blood occlusion
Notch signaling may negatively regulate neonatal rat cardiac fibroblast-myofibroblast transformation
Identification of mRNAs related to endometrium function regulated by lncRNA CD36%u2013005 in rat endometrial stromal cells
Expression of Wnt/?-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-?1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model
Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis
P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3
Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor ? and ?
Expression of pannexin 1 and 2 in cortical lesions from intractable epilepsy patients with focal cortical dysplasia
Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress
Wu Y, Wang Y, Ji Y, Ou Y, Xia H, Zhang B, Zhao Y. Am J Transl Res. 2017 Dec 15;9(12):5708-5718. eCollection 2017. C4orf7 modulates osteogenesis and adipogenesis of human periodontal ligament cells
Gu X, Fang T, Kang P, Hu J, Yu Y, Li Z, Cheng X, Gao Q. Oxid Med Cell Longev. 2017;2017:9257967. doi: 10.1155/2017/9257967. Epub 2017 Oct 9. Effect of ALDH2 on High Glucose-Induced Cardiac Fibroblast Oxidative Stress, Apoptosis, and Fibrosis
Ling R, Zhou Y, Zhou L, Dai D, Wu D, Mi L, Mao C, Chen D. Mol Med Rep. 2018 Apr;17(4):5265-5271. doi: 10.3892/mmr.2018.8548. Epub 2018 Feb 2. Lin28/microRNA-let-7a promotes metastasis under circumstances of hyperactive Wnt signaling in esophageal ...
Shen Z, Lu D, Li Q, Zhang Z, Zhu Y. Biomed Res Int. 2015;2015:871202. Doi: 10.1155/2015/871202. Epub 2015 Mar 8. Synthesis And Characterization Of Biodegradable Polyurethane For Hypopharyngeal Tissue Engineering.
Yuan Y, Su Z, Pu Y, Liu X, Chen J, Zhu F, Zhu Y, Zhang H, He C. Br J Pharmacol. 2012 May;166(2):749-63. Doi: 10.1111/J.1476-5381.2011.01804.X. Ethyl Pyruvate Promotes Spinal Cord Repair By Ameliorating The Glial Microenvironment.
Han X, Fang X, Lou X, Hua D, Ding W, Foltz G, Hood L, Yuan Y, Lin B. Plos One. 2012;7(8):E41335. Doi: 10.1371/Journal.Pone.0041335. Epub 2012 Aug 17. Silencing Sox2 Induced Mesenchymal-Epithelial Transition And Its Expression Predicts Liver And Ly...
Wang J, Feng X, Zeng Y, Fan J, Wu J, Li Z, Liu X, Huang R, Huang F, Yu X, Yang X. Bmc Microbiol. 2013 Nov 13;13:255. Doi: 10.1186/1471-2180-13-255. Lipopolysaccharide (Lps)-Induced Autophagy Is Involved In The Restriction Of Escherichia Coli In Pe...
Xu B,, Jin X,, Min L, Li Q,, Deng L,, Wu H, Lin G, Chen L, Zhang H, Li C, Wang L, Zhu J,, Wang W,, Chu F,, Shen J,, Li H, Mao J,. Oncotarget. 2015 Feb 10;6(4):2434-50. Chloride Channel-3 Promotes Tumor Metastasis By Regulating Membrane Ruffling An...
Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.
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