Anti-Vimentin Antibody

Boster Bio Anti-Vimentin Antibody catalog # M00235-5. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat. Cited in 31 publication(s).

Product Info Summary

SKU: M00235-5
Size: 100ul
Reactive Species: Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
Host: Chicken
Application: IF, IHC, ICC, WB

Product Name

Anti-Vimentin Antibody

See all Vimentin products

SKU/Catalog Number

M00235-5

Size

100ul

Description

Boster Bio Anti-Vimentin Antibody catalog # M00235-5. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.

Storage & Handling

Stable at 4 ° C for at least one year

Cite This Product

Anti-Vimentin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00235-5)

Host

Chicken

Contents

Supplied as an aliquot of IgY preparation plus 5mM NaN3

Clonality

Polyclonal

Immunogen

Full length recombinant human vimentin protein , PROT-r-Vim, expressed in and purified from E. coli.

Reactive Species

M00235-5 is reactive to VIM in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat

Applications

M00235-5 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB: 1:10000.
IF/ICC and IHC: 1:5000.

Validation Images & Assay Conditions

Gene/Protein Information For VIM (Source: Uniprot.Org, NCBI)

Uniprot ID

P08670

Gene ID

7431

Gene Name

VIM

Full Name

Vimentin

Weight

53.652kDa

Superfamily

intermediate filament family

Alternative Names

FLJ36605; VIM; Vimentin VIM vimentin vimentin|epididymis secretory sperm binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VIM, check out the VIM Infographic

VIM infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VIM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

M00235-5 has been cited in 31 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu R,Xu B,Yu S,Zhang J,Sun H,Liu C,Lu F,Pan Q,Zhang X.Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts and Mycoplasma synoviae.Front Microbiol.2020 Apr 3;11:576.doi:10.3389/fmicb.2020.00576.PMID:323
Species: Chicken
M00235-5 usage in article: APP:IF, SAMPLE:CSF, DILUTION:NA

Zhang XW,Li QH,Xu ZD,Dou JJ.STAT1-induced regulation of lncRNA ZFPM2-AS1 predicts poor prognosis and contributes to hepatocellular carcinoma progression via the miR-653/GOLM1 axis.Cell Death Dis.2021 Jan 4;12(1):31.doi:10.1038/s41419-020-03300-4.PMID:3341
Species: Human,Mouse
M00235-5 usage in article: APP:WB, SAMPLE:HCC CELL, DILUTION:1:800

Jue-Ying Chen,Ji-Wei Qiu,Jun-Lin Mo,et al.Effects of Yishenbupi (tonifying-kidney and invigorating-spleen) prescription on the expression of renal fibrosis-associated proteins in unilateral ureteral occlusion rats.TMR Integrative Medicine 2020,4:e20012.
Species: Rat
M00235-5 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000

Jiang SB,Lu YS,Liu T,Li LM,Wang HX,Wu Y,Gao XH,Chen HD. UVA influenced the SIRT1-miR-27a-5p-SMAD2-MMP1/COL1/BCL2 axis in human skin primary fibroblasts.J Cell Mol Med.2020 Sep;24(17):10027-10041.doi: 10.1111/jcmm.15610.Epub 2020 Aug 13.PMID:32790210;PMCID
Species: Human
M00235-5 usage in article: APP:IF, SAMPLE:SKIN PRIMARY FIBROBLASTS, DILUTION:1:100

The Role of the RhoA/ROCK Signaling Pathway in Mechanical Strain-Induced Scleral Myofibroblast Differentiation

Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments

Autophagy Is Indispensable for the Self-Renewal and Quiescence of Ovarian Cancer Spheroid Cells with Stem Cell-Like Properties

Expression of E-cadherin and vimentin in oral squamous cell carcinoma

%u03B22-microglobulin induces epithelial-mesenchymal transition in human renal proximal tubule epithelial cells in vitro

Distinct Roles For ROCK1 and ROCK2 in the Regulation of Oxldl-Mediated Endothelial Dysfunction

MTDH promotes glioma invasion through regulating miR-130b-ceRNAs

Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human %u2026

Mesenchymal Stem Cells Promote Tumor Progression via Inducing Stroma Remodeling on Rabbit VX2 Bladder Tumor Model

Changes in hippocampal ultrastructure and vimentin expression in rhesus monkeys following selective deep hypothermia and blood occlusion

Notch signaling may negatively regulate neonatal rat cardiac fibroblast-myofibroblast transformation

Identification of mRNAs related to endometrium function regulated by lncRNA CD36%u2013005 in rat endometrial stromal cells

Expression of Wnt/?-Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF-?1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model

Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis

P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3

Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor ? and ?

Expression of pannexin 1 and 2 in cortical lesions from intractable epilepsy patients with focal cortical dysplasia

Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress

Wu Y, Wang Y, Ji Y, Ou Y, Xia H, Zhang B, Zhao Y. Am J Transl Res. 2017 Dec 15;9(12):5708-5718. eCollection 2017. C4orf7 modulates osteogenesis and adipogenesis of human periodontal ligament cells

Gu X, Fang T, Kang P, Hu J, Yu Y, Li Z, Cheng X, Gao Q. Oxid Med Cell Longev. 2017;2017:9257967. doi: 10.1155/2017/9257967. Epub 2017 Oct 9. Effect of ALDH2 on High Glucose-Induced Cardiac Fibroblast Oxidative Stress, Apoptosis, and Fibrosis

Ling R, Zhou Y, Zhou L, Dai D, Wu D, Mi L, Mao C, Chen D. Mol Med Rep. 2018 Apr;17(4):5265-5271. doi: 10.3892/mmr.2018.8548. Epub 2018 Feb 2. Lin28/microRNA-let-7a promotes metastasis under circumstances of hyperactive Wnt signaling in esophageal ...

Shen Z, Lu D, Li Q, Zhang Z, Zhu Y. Biomed Res Int. 2015;2015:871202. Doi: 10.1155/2015/871202. Epub 2015 Mar 8. Synthesis And Characterization Of Biodegradable Polyurethane For Hypopharyngeal Tissue Engineering.

Yuan Y, Su Z, Pu Y, Liu X, Chen J, Zhu F, Zhu Y, Zhang H, He C. Br J Pharmacol. 2012 May;166(2):749-63. Doi: 10.1111/J.1476-5381.2011.01804.X. Ethyl Pyruvate Promotes Spinal Cord Repair By Ameliorating The Glial Microenvironment.

Han X, Fang X, Lou X, Hua D, Ding W, Foltz G, Hood L, Yuan Y, Lin B. Plos One. 2012;7(8):E41335. Doi: 10.1371/Journal.Pone.0041335. Epub 2012 Aug 17. Silencing Sox2 Induced Mesenchymal-Epithelial Transition And Its Expression Predicts Liver And Ly...

Wang J, Feng X, Zeng Y, Fan J, Wu J, Li Z, Liu X, Huang R, Huang F, Yu X, Yang X. Bmc Microbiol. 2013 Nov 13;13:255. Doi: 10.1186/1471-2180-13-255. Lipopolysaccharide (Lps)-Induced Autophagy Is Involved In The Restriction Of Escherichia Coli In Pe...

Xu B,, Jin X,, Min L, Li Q,, Deng L,, Wu H, Lin G, Chen L, Zhang H, Li C, Wang L, Zhu J,, Wang W,, Chu F,, Shen J,, Li H, Mao J,. Oncotarget. 2015 Feb 10;6(4):2434-50. Chloride Channel-3 Promotes Tumor Metastasis By Regulating Membrane Ruffling An...

Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.

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16 Customer Q&As for Anti-Vimentin Antibody

Question

We need using your anti-Vimentin antibody for striated muscle contraction studies. Has this antibody been tested with western blotting on neuronal lineage cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

I appreciate your inquiry. This M00235-5 anti-Vimentin antibody is tested on neuronal lineage cells. It is guaranteed to work for IHC, WB in bovine, chicken, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-18

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Vimentin antibody M00235-5. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-07

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-07

Question

We are currently using anti-Vimentin antibody M00235-5 for chicken tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says bovine, chicken, equine, human, mouse, pig, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

The anti-Vimentin antibody (M00235-5) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-03

Question

I was wanting to use your anti-Vimentin antibody for WB for pig liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for pig liver identification?

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

As indicated on the product datasheet, M00235-5 anti-Vimentin antibody has been tested for IHC, WB on bovine, chicken, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in pig liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-07

Question

We are interested in to test anti-Vimentin antibody M00235-5 on pig liver for research purposes, then I may be interested in using anti-Vimentin antibody M00235-5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

The products we sell, including anti-Vimentin antibody M00235-5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-11

Question

Would M00235-5 anti-Vimentin antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

It shows on the product datasheet, M00235-5 anti-Vimentin antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-07

Question

My colleagues were satisfied with the WB result of your anti-Vimentin antibody. However we have seen positive staining in cajal-retzius cell fetal brain cortex cytoplasm using this antibody. Is that expected? Could you tell me where is VIM supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

From literature, cajal-retzius cell fetal brain cortex does express VIM. Generally VIM expresses in cytoplasm. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579

Boster Scientific Support

Answered: 2019-06-11

Question

Is this M00235-5 anti-Vimentin antibody reactive to the isotypes of VIM?

Verified Customer

Verified customer

Asked: 2019-01-31

Answer

The immunogen of M00235-5 anti-Vimentin antibody is Full length recombinant human vimentin protein , PROT-r-Vim, expressed in and purified from E. coli. . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-01-31

Question

Please see the WB image, lot number and protocol we used for liver using anti-Vimentin antibody M00235-5. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-10-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-03

Question

I see that the anti-Vimentin antibody M00235-5 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-05-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-05-28

Question

Do you have a BSA free version of anti-Vimentin antibody M00235-5 available?

J. Edwards

Verified customer

Asked: 2018-01-04

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Vimentin antibody M00235-5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-01-04

Question

Is a blocking peptide available for product anti-Vimentin antibody (M00235-5)?

H. Anderson

Verified customer

Asked: 2017-12-14

Answer

We do provide the blocking peptide for product anti-Vimentin antibody (M00235-5). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-12-14

Question

Would anti-Vimentin antibody M00235-5 work for WB with liver?

Verified Customer

Verified customer

Asked: 2017-10-31

Answer

According to the expression profile of liver, VIM is highly expressed in liver. So, it is likely that anti-Vimentin antibody M00235-5 will work for WB with liver.

Boster Scientific Support

Answered: 2017-10-31

Question

Our lab used your anti-Vimentin antibody for IHC on lymphoblast last year. I am using human, and I plan to use the antibody for WB next. We need examining lymphoblast as well as cervix carcinoma erythroleukemia in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

T. Johnson

Verified customer

Asked: 2017-01-30

Answer

I looked at the website and datasheets of our anti-Vimentin antibody and it seems that M00235-5 has been tested on human in both IHC and WB. Thus M00235-5 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-01-30

Question

My question regarding product M00235-5, anti-Vimentin antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Carter

Verified customer

Asked: 2014-05-09

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00235-5 anti-Vimentin antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-05-09

Question

We have seen staining in mouse cajal-retzius cell fetal brain cortex. Do you have any suggestions? Is anti-Vimentin antibody supposed to stain cajal-retzius cell fetal brain cortex positively?

D. Roberts

Verified customer

Asked: 2014-04-08

Answer

From what I have seen in literature cajal-retzius cell fetal brain cortex does express VIM. From what I have seen in Uniprot.org, VIM is expressed in dorsal root ganglion, lymphoma, testis, embryo, placenta stomach, adipose tissue coronary artery, cervix, placenta testis, mammary carcinoma, t-cell, hepatoma, brain, cajal-retzius cell fetal brain cortex, fibroblast, osteosarcoma, lymphoblast, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have VIM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 17924679, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryo, Placenta, and Stomach, Pubmed ID: 14702039
Fibroblast, Pubmed ID: 3371665
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Lymphoma, Pubmed ID: 14996095
Mammary carcinoma, Pubmed ID: 9150946
Osteosarcoma, Pubmed ID: 2323579

Boster Scientific Support

Answered: 2014-04-08

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M00235-5
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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