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SKU:MA1102
Clonality:Monoclonal
Clone Number:V9
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Vimentin Antibody (Monoclonal, V9)
Description Mouse IgG monoclonal antibody for Vimentin, vimentin (VIM) detection. Tested with WB, IHC-P in Human;rat;rabbit;pig. No cross reactivity with other proteins.
Cite This Product Anti-Vimentin Antibody (Monoclonal, V9) V9 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1102)
Replacement Item This antibody may replace the following items: sc-32482|sc-98936 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Pig, Rabbit, Rat
Predicted Species Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Pig eye lens vimentin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number V9
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Vim
Protein Name Vimentin
Molecular Weight 53733 MW
Protein Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. .
Tissue Specificity VIM: Highly expressed in fibroblasts, someexpression in T- and B-lymphocytes, and little or no expression inBurkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Sequence Similarities Belongs to the intermediate filament family.
Subcellular Localization Nucleus matrix . Cytoplasm .
Uniprot ID P31000
Alternative Names Vimentin;Vim;
Research Areas |neuroscience|cell type marker|neural stem cell marker| signal transduction|cytoskeleton / ecm|cytoskeleton|intermediate filaments|class iii|vimentin| neuroscience|neurology process|neurogenesis| stem cells|lineage markers|endoderm|neural stem cells|intracellular| cancer|tumor biomarkers|neuroregeneration| developmental biology|lineage specification|ectoderm|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Vimentin

The VIM gene was one of many that Gieser and Swaroop(1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.

Anti-Vimentin Antibody (Monoclonal, V9) Images

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Anti-Vimentin Antibody (Monoclonal, V9)
Anti-Vimentin antibody (monoclonal), MA1102, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Publications

Zhai W, Wang N, Qi Z, Gao Q, Yi L. Orthopedics. 2012 Apr;35(4):E520-5. Doi: 10.3928/01477447-20120327-22. Platelet-Rich Plasma Reverses The Inhibition Of Tenocytes And Osteoblasts In Tendon-Bone Healing.
Huang F, Wang H, Zou Y, Liu Q, Cao J, Yin T. Int J Clin Exp Pathol. 2013 Oct 15;6(11):2487-96. Ecollection 2013. Effect Of Gnrh-Ii On The Esc Proliferation, Apoptosis And Vegf Secretion In Patients With Endometriosis In Vitro.
Shen Z, Lu D, Li Q, Zhang Z, Zhu Y. Biomed Res Int. 2015;2015:871202. Doi: 10.1155/2015/871202. Epub 2015 Mar 8. Synthesis And Characterization Of Biodegradable Polyurethane For Hypopharyngeal Tissue Engineering.
Em S, Kataria M, Shah F, Yadav Ps. Cytotechnology. 2014 Dec 13. [Epub Ahead Of Print] A Comparative Study On Efficiency Of Adult Fibroblasts And Amniotic Fluid-Derived Stem Cells As Donor Cells For Production Of Hand-Made Cloned Buffalo (Bubalus B...
Xia Yy, Yin L, Jiang N, Guo Wj, Tian H, Jiang Xs, Wu J, Chen M, Wu Jz, He X. Biochem Biophys Res Commun. 2015 Jul 31;463(3):357-63. Doi: 10.1016/J.Bbrc.2015.05.068. Epub 2015 May 27. Downregulating Hmga2 Attenuates Epithelial-Mesenchymal Transitio...
Su Z, Yuan Y, Cao L, Zhu Y, Gao L, Qiu Y, He C. Glia. 2010 Jun;58(8):901-15. Doi: 10.1002/Glia.20972. Triptolide Promotes Spinal Cord Repair By Inhibiting Astrogliosis And Inflammation.
Yuan Y, Su Z, Pu Y, Liu X, Chen J, Zhu F, Zhu Y, Zhang H, He C. Br J Pharmacol. 2012 May;166(2):749-63. Doi: 10.1111/J.1476-5381.2011.01804.X. Ethyl Pyruvate Promotes Spinal Cord Repair By Ameliorating The Glial Microenvironment.
Li R, Li X, Zhou M, Han N, Zhang Q. Arch Oral Biol. 2012 Oct;57(10):1408-17. Doi: 10.1016/J.Archoralbio.2012.07.005. Epub 2012 Aug 4. Quantitative Determination Of Matrix Gla Protein (Mgp) And Bmp-2 During The Osteogenic Differentiation Of Human P...
Shi Y, Dong Y, Duan Y, Jiang X, Chen C, Deng L. Mol Med Rep. 2013 Feb;7(2):419-24. Doi: 10.3892/Mmr.2012.1213. Epub 2012 Dec 3. Substrate Stiffness Influences Tgf-??1-Induced Differentiation Of Bronchial Fibroblasts Into Myofibroblasts In Airway Re...
Fan L, Hu K, Ji K, Sun Q, Xiong J, Yang L, Liu H. In Vitro Cell Dev Biol Anim. 2014 Apr;50(4):304-12. Doi: 10.1007/S11626-013-9701-6. Epub 2013 Oct 26. Directed Differentiation Of Aged Human Bone Marrow Multipotent Stem Cells Effectively Generates...
Huang Q, Jin H, Xie Z, Wang M, Chen J, Zhou Y. J Int Med Res. 2013 Aug;41(4):1242-51. Doi: 10.1177/0300060513493995. Epub 2013 Jul 18. The Role Of The Erk1/2 Signalling Pathway In The Pathogenesis Of Female Stress Urinary Incontinence.
Su W, Wang P, Chen H, Li H. J Endocrinol Invest. 2014 Jun;37(6):559-64. Doi: 10.1007/S40618-014-0068-7. Epub 2014 Apr 11. Role Of Protein Kinase C ??
Chen B, Sun Hh, Wang Hg, Kong H, Chen Fm, Yu Q. Biomaterials. 2012 Jul;33(20):5023-35. Doi: 10.1016/J.Biomaterials.2012.03.057. Epub 2012 Apr 18. The Effects Of Human Platelet Lysate On Dental Pulp Stem Cells Derived From Impacted Human Third Molars.
Zhang J, Wang Z, Jiang Y, Niu Z, Fu L, Luo Z, Cooper Pr, Smith Aj, He W. Exp Cell Res. 2015 Mar 15;332(2):259-66. Doi: 10.1016/J.Yexcr.2015.01.020. Epub 2015 Feb 8. Nuclear Factor I-C Promotes Proliferation And Differentiation Of Apical Papilla-De...
Wang P, Wang X, Liu Q, Zhao X, Cao B, Zhao P. Cancer Biother Radiopharm. 2010 Feb;25(1):55-64. Doi: 10.1089/Cbr.2008.0604. Comparision Between Sonodynamic Effects With Protoporphyrin Ix And Hematoporphyrin On The Cytoskeleton Of Ehrlich Ascites Ca...
Zhang H, Liu X, Chen S, Wu J, Ye X, Xu L, Chen H, Zhang D, Tan R, Wang Y. J Ethnopharmacol. 2010 Aug 19;131(1):165-73. Doi: 10.1016/J.Jep.2010.06.022. Epub 2010 Jun 19. Tectorigenin Inhibits The In Vitro Proliferation And Enhances Mir-338* Express...
Ren M, Wang B, Zhang J, Liu P, Lv Y, Liu G, Jiang H, Liu F. Mol Med Rep. 2011 May-Jun;4(3):561-7. Doi: 10.3892/Mmr.2011.458. Epub 2011 Mar 18. Smad2 And Smad3 As Mediators Of The Response Of Adventitial Fibroblasts Induced By Transforming Growth F...
Li S, Zhou Y, Fan J, Cao S, Cao T, Huang F, Zhuang S, Wang Y, Yu X, Mao H. Am J Pathol. 2011 Dec;179(6):2822-34. Doi: 10.1016/J.Ajpath.2011.08.013. Epub 2011 Oct 11. Heat Shock Protein 72 Enhances Autophagy As A Protective Mechanism In Lipopolysac...
An S, Ling J, Gao Y, Xiao Y. J Periodontal Res. 2012 Jun;47(3):374-82. Doi: 10.1111/J.1600-0765.2011.01443.X. Epub 2011 Dec 5. Effects Of Varied Ionic Calcium And Phosphate On The Proliferation, Osteogenic Differentiation And Mineralization Of Hum...
Chen P, Mo Q, Wang B, Weng D, Wu P, Chen G. J Huazhong Univ Sci Technolog Med Sci. 2012 Feb;32(1):92-6. Doi: 10.1007/S11596-012-0016-8. Epub 2012 Jan 27. Breast Cancer Associated Fibroblasts Promote Mcf-7 Invasion In Vitro By Secretion Of Hgf.
Fu G, Che X, Sun Y, Huang X, Xu H, Zhou C, Zhang X. Acta Histochem. 2013 May;115(4):301-7. Doi: 10.1016/J.Acthis.2012.08.006. Epub 2012 Sep 10. Pigment Epithelial-Derived Factor Expression In Endometriotic Lesions In A Rat Model Of Endometriosis.
Han X, Fang X, Lou X, Hua D, Ding W, Foltz G, Hood L, Yuan Y, Lin B. Plos One. 2012;7(8):E41335. Doi: 10.1371/Journal.Pone.0041335. Epub 2012 Aug 17. Silencing Sox2 Induced Mesenchymal-Epithelial Transition And Its Expression Predicts Liver And Ly...
Li F, Cai Z, Chen F, Shi X, Zhang Q, Chen S, Shi J, Wang Dw, Dong N. Basic Res Cardiol. 2012 Nov;107(6):306. Doi: 10.1007/S00395-012-0306-0. Epub 2012 Oct 16. Pioglitazone Attenuates Progression Of Aortic Valve Calcification Via Down-Regulating Re...
Qi X, Qu Y, Nan Z, Jin Y, Zhao X, Wang A. Reprod Biol. 2012 Nov;12(3):309-15. Doi: 10.1016/J.Repbio.2012.09.003. Epub 2012 Oct 17. Caprine Endometrial Stromal Cells Modulate The Effects Of Steroid Hormones On Cytokine Secretion By Endometrial Epit...
Yijing L, Liu H, Yuan C, Wang B, Ren M, Yan L, Wang X, Zhang J. Pharm Biol. 2013 Jun;51(6):712-21. Doi: 10.3109/13880209.2013.764328. Epub 2013 Feb 4. The Effects Of Qindan-Capsule-Containing Serum On The Tgf-??1/Erk Signaling Pathway, Matrix Metal...
Wang J, Feng X, Zeng Y, Fan J, Wu J, Li Z, Liu X, Huang R, Huang F, Yu X, Yang X. Bmc Microbiol. 2013 Nov 13;13:255. Doi: 10.1186/1471-2180-13-255. Lipopolysaccharide (Lps)-Induced Autophagy Is Involved In The Restriction Of Escherichia Coli In Pe...
Zhou Qd, Ning Y, Zeng R, Chen L, Kou P, Xu Co, Pei Gc, Han M, Xu G. J Huazhong Univ Sci Technolog Med Sci. 2013 Oct;33(5):672-9. Doi: 10.1007/S11596-013-1179-7. Epub 2013 Oct 20. Erbin Interacts With Sema4C And Inhibits Sema4C-Induced Epithelial-M...
Jiang Y, Kou Z, Wu T, An W, Zhou R, Wang H, Gao Y, Gao S. Stem Cells Dev. 2014 Oct 1;23(19):2283-96. Doi: 10.1089/Scd.2014.0011. Epub 2014 Jun 26. Xist Deficiency And Disorders Of X-Inactivation In Rabbit Embryonic Stem Cells Can Be Rescued By Tra...
Yao W, Huang C, Sun Q, Jing X, Wang H, Zhang W. Cell Physiol Biochem. 2014;34(5):1442-54. Doi: 10.1159/000366349. Epub 2014 Oct 6. Tetrahydroxystilbene Glucoside Protects Against Oxidized Ldl-Induced Endothelial Dysfunction Via Regulating Vimentin...
Jiang W, Lv H, Wang H, Wang D, Sun S, Jia Q, Wang P, Song B, Ni L. Cell Tissue Res. 2015 Aug;361(2):541-55. Doi: 10.1007/S00441-015-2118-7. Epub 2015 Feb 17. Activation Of The Nlrp3/Caspase-1 Inflammasome In Human Dental Pulp Tissue And Human Dent...
Bao Ch, Wang Xt, Ma W, Wang Nn, Un Nesa E, Wang Jb, Wang C, Jia Yb, Wang K, Tian H, Cheng Yf. Biochem Biophys Res Commun. 2015 Mar 6;458(2):441-7. Doi: 10.1016/J.Bbrc.2015.02.001. Epub 2015 Feb 10. Irradiated Fibroblasts Promote Epithelial-Mesench...
Su F,, Liu Ss,, Ma Jl, Wang Ds, E Ll, Liu Hc. Stem Cell Res Ther. 2015 Mar 12;6:22. Doi: 10.1186/S13287-015-0023-3. Enhancement Of Periodontal Tissue Regeneration By Transplantation Of Osteoprotegerin-Engineered Periodontal Ligament Stem Cells.
Wang X, Zou F, Deng H, Fu Z, Li Y, Wu L, Wang Z, Liu L. Mol Med Rep. 2014 Dec;10(6):2937-41. Doi: 10.3892/Mmr.2014.2601. Epub 2014 Sep 26. Characterization Of Sphere?
Zhang A, Wang B, Yang M, Shi H, Gan W. Bmc Nephrol. 2015 Apr 23;16:60. Doi: 10.1186/S12882-015-0057-X. ??2-Microglobulin Induces Epithelial-Mesenchymal Transition In Human Renal Proximal Tubule Epithelial Cells In Vitro.
Xu B,, Jin X,, Min L, Li Q,, Deng L,, Wu H, Lin G, Chen L, Zhang H, Li C, Wang L, Zhu J,, Wang W,, Chu F,, Shen J,, Li H, Mao J,. Oncotarget. 2015 Feb 10;6(4):2434-50. Chloride Channel-3 Promotes Tumor Metastasis By Regulating Membrane Ruffling An...
Yang D, L?? X, Hong Y, Xi T, Zhang D. Biomaterials. 2014 Aug;35(24):6195-205. Doi: 10.1016/J.Biomaterials.2014.04.069. Epub 2014 May 10. The Molecular Mechanism For Effects Of Tin Coating On Niti Alloy On Endothelial Cell Function.
Han P, Fu Y, Luo M, He J, Liu J, Liao J, Tian D, Yan W. Dig Dis Sci. 2014 May;59(5):992-1000. Doi: 10.1007/S10620-013-2992-3. Epub 2014 Jan 18. Bves Inhibition Triggers Epithelial-Mesenchymal Transition In Human Hepatocellular Carcinoma.
Ren Y, Wu H, Zhou X, Wen J, Jin M, Cang M, Guo X, Wang Q, Liu D, Ma Y. Res Vet Sci. 2012 Aug;93(1):404-11. Doi: 10.1016/J.Rvsc.2011.08.014. Epub 2011 Sep 25. Isolation, Expansion, And Differentiation Of Goat Adipose-Derived Stem Cells.
Duan F, Lin M, Li C, Ding X, Qian G, Zhang H, Ge S, Fan X, Li J. Cancer Biol Ther. 2014 May;15(5):544-59. Doi: 10.4161/Cbt.28157. Epub 2014 Mar 11. Effects Of Inhibition Of Hedgehog Signaling On Cell Growth And Migration Of Uveal Melanoma Cells.
Jiang Zy, Guo Yy, Ren Hb, Zou Yf, Fan Ms, Lv Y, Han P, De W, Sun Lz. Placenta. 2012 Jan;33(1):1-7. Doi: 10.1016/J.Placenta.2011.09.004. Epub 2011 Nov 1. Tumor Necrosis Factor (Tnf)-?? Upregulates Progesterone Receptor-A By Activating The Nf-??b Sign...
Gai X, Lu Z, Tu K, Liang Z, Zheng X. Plos One. 2014 Jan 13;9(1):E84551. Doi: 10.1371/Journal.Pone.0084551. Ecollection 2014. Caveolin-1 Is Up-Regulated By Gli1 And Contributes To Gli1-Driven Emt In Hepatocellular Carcinoma.
Xia H, Cheung Wk, Sze J, Lu G, Jiang S, Yao H, Bian Xw, Poon Ws, Kung Hf, Lin Mc. J Biol Chem. 2010 Nov 19;285(47):36995-7004. Doi: 10.1074/Jbc.M110.133744. Epub 2010 Sep 7. Mir-200A Regulates Epithelial-Mesenchymal To Stem-Like Transition Via Zeb...
Zhu Xl, Wang Yj, Yang Y??, Yang Rc, Zhu B, Zhang Y??, Lin Y, Lu Y, Li Xf, O'Byrne Kt. Mol Med Rep. 2012 Sep;6(3):493-500. Doi: 10.3892/Mmr.2012.960. Epub 2012 Jun 21. Suppression Of Lipopolysaccharide-Induced Upregulation Of Toll-Like Re...
Liang Xj, Chen Xj, Yang Dh, Huang Sm, Sun Gd, Chen Yp. Cell Biol Int. 2012 Feb;36(2):215-21. Doi: 10.1042/Cbi20110350. Differentiation Of Human Umbilical Cord Mesenchymal Stem Cells Into Hepatocyte-Like Cells By Htert Gene Transfection In Vitro.
Zhang X, Shi K, Li Y, Zhang H, Hao J. Reprod Toxicol. 2014 Jun;45:87-93. Doi: 10.1016/J.Reprotox.2014.01.009. Epub 2014 Feb 4. Lipopolysaccharide Inhibits The Self-Renewal Of Spermatogonial Stem Cells In Vitro Via Downregulation Of Gdnf Expression...
Chen Q, Zhang Z, Liu J, He Q, Zhou Y, Shao G, Sun X, Cao X, Gong A, Jiang P. Mol Cells. 2015 Mar;38(3):221-8. Doi: 10.14348/Molcells.2015.2170. Epub 2015 Feb 4. A Fibrin Matrix Promotes The Differentiation Of Emscs Isolated From Nasal Respiratory ...
Zhang C, Peng Y, Wang F, Tan X, Liu N, Fan S, Wang D, Zhang L, Liu D, Wang T, Wang S, Zhou Y, Su Y, Cheng T, Zhuang Z, Shi C. Biomaterials. 2010 Dec;31(36):9535-43. Doi: 10.1016/J.Biomaterials.2010.08.059. Epub 2010 Sep 26. A Synthetic Cantharidin...
Bai J, Liu H, Li D, Cui L, Wu X. Plos One. 2012;7(11):E49673. Doi: 10.1371/Journal.Pone.0049673. Epub 2012 Nov 20. Duodenum Clamping Trauma Induces Significant Postoperative Intraperitoneal Adhesions On A Rat Model.
Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.
Qiao B, Johnson Nw, Gao J. Int J Oncol. 2010 Sep;37(3):663-8. Epithelial-Mesenchymal Transition In Oral Squamous Cell Carcinoma Triggered By Transforming Growth Factor-Beta1 Is Snail Family-Dependent And Correlates With Matrix Metalloproteinase-2 ...
Wu Y, Chen W, Guo M, He Q, Hu Y. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4827-36. Ecollection 2014. Effects Of Transforming Growth Factor-??2 On Myocilin Expression And Secretion In Human Primary Cultured Trabecular Meshwork Cells.
Pan X, Chen Z, Huang R, Yao Y, Ma G. Plos One. 2013;8(4):E60335. Doi: 10.1371/Journal.Pone.0060335. Epub 2013 Apr 1. Transforming Growth Factor ??1 Induces The Expression Of Collagen Type I By Dna Methylation In Cardiac Fibroblasts.
Jiang R, Xu W, Zhu W, Chen M, Qian H, Qiao C, Yang H, Wang X, Chen Y. Oncol Rep. 2006 Nov;16(5):1021-8. Histological Type Of Oncogenity And Expression Of Cell Cycle Genes In Tumor Cells From Human Mesenchymal Stem Cells.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to vimentin antibody, vim antibody