|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-Vinculin Antibody (Monoclonal, hVIN-1)|
|Description||Mouse IgG monoclonal antibody for Vinculin, vinculin (VCL) detection. Tested with WB, IHC-F in Human;mouse;rat;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Vinculin Antibody (Monoclonal, hVIN-1) hVIN-1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1103)|
|Replacement Item||This antibody may replace the following items: sc-25336|sc-55465|sc-5573|sc-59803|sc-73264|sc-73614|sc-7648|sc-7649 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Mouse, Rat|
|Predicted Species||Bovine, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Human vinculin, purified from uterus.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||116615 MW|
|Protein Function||Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity). .|
|Tissue Specificity||VCL: Metavinculin is muscle-specific.|
|Sequence Similarities||Belongs to the vinculin/alpha-catenin family.|
|Subcellular Localization||Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. .|
|Alternative Names||Vinculin ;Metavinculin ;Vcl ;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins| cardiovascular|heart|cardiac arrhythmias|contractility|contractile proteins|actins| isotype/loading controls|vinculin||
Background for Vinculin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Vinculin Antibody (Monoclonal, hVIN-1) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 2-4μg/ml, Human, chicken, mouse, rat, -|
Western blot, 1-2μg/ml, Human, chicken, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Vinculin Antibody (Monoclonal, hVIN-1) Images
Click the images to enlarge.
IHC(F): Human Placenta Tissue
IHC(F): Human Placenta Tissue