Data & Images
|Product Name||Anti-VR1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Transient receptor potential cation channel subfamily V member 1(TRPV1) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-VR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1030)|
|Replacement Item||This antibody may replace the following items: sc-367637 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived human VR1 recombinant protein (Position: F473-K839). Human VR1 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat VR1, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Transient receptor potential cation channel subfamily V member 1|
|Molecular Weight||94956 MW|
|Protein Function||Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Can be activated by endogenous compounds, including 12- hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (By similarity). Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. .|
|Tissue Specificity||Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level). .|
|Sequence Similarities||Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.|
|Subcellular Localization||Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Cell projection, dendritic spine membrane ; Multi- pass membrane protein . Cell membrane ; Multi-pass membrane protein . Mostly, but not exclusively expressed in postsynaptic dendritic spines. .|
|Alternative Names||Transient receptor potential cation channel subfamily V member 1;TrpV1;Capsaicin receptor;Osm-9-like TRP channel 1;OTRPC1;Vanilloid receptor 1;TRPV1;VR1;|
Background for Transient receptor potential cation channel subfamily V member 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-VR1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-VR1 Antibody Images
Click the images to enlarge.
IHC(P): Human Tonsil Tissue
IHC(P): Human ESCC Tissue
All lanes: Anti-VR1(RP1030) at 0.5ug/ml
WB: Recombinant Human VR1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,