Anti-Von Willebrand Factor/VWF Antibody Picoband™

VWF antibody

Boster Bio Anti-Von Willebrand Factor/VWF Antibody Picoband™ catalog # PB9062. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: PB9062
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IHC, IHC-F, ICC, WB

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Product Name

Anti-Von Willebrand Factor/VWF Antibody Picoband™

View all VWF Antibodies

SKU/Catalog Number

PB9062

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Von Willebrand Factor/VWF Antibody Picoband™ catalog # PB9062. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Von Willebrand Factor/VWF Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9062)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human VWF recombinant protein (Position: R2535-K2813). Human VWF shares 79% amino acid (aa) sequence identity with mouse VWF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9062 is reactive to VWF in Human

Applications

PB9062 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

309 kDa

Calculated molecular weight

309.265kDa

Background of VWF

Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For VWF (Source: Uniprot.org, NCBI)

Gene Name

VWF

Full Name

von Willebrand factor

Weight

309.265kDa

Alternative Names

coagulation factor VIII VWF; F8; F8VWF; von Willebrand factor; VWD; vWF VWF F8VWF, VWD von Willebrand factor von Willebrand factor|coagulation factor VIII VWF

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on VWF, check out the VWF Infographic

VWF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for VWF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9062 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Fibroblast growth factor 21 delayed endothelial replicative senescence and protected cells from H2O2-induced premature senescence through SIRT1

Identification and characterization of cancer stem-like cells from primary carcinoma of the cervix uteri

Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells

Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells

Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro

PlexinA1 expression in gastric carcinoma and its relationship with tumor angiogenesis and proliferation

A Three-Dimensional Printed Polycaprolactone Scaffold Combined with Co-Axially Electrospun Vancomycin/Ceftazidime/Bone Morphological Protein-2 Sheath-Core Nanofibers for the Repair of Segmental Bone Defects During the Masquelet Procedure

IFI6 Inhibits Apoptosis via Mitochondrial-Dependent Pathway in Dengue Virus 2 Infected Vascular Endothelial Cells

The protective effects of angelica organic acid against ox-LDL-induced autophagy dysfunction of HUVECs

RoY Peptide-Modified Chitosan-Based Hydrogel to Improve Angiogenesis and Cardiac Repair under Hypoxia

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16 Customer Q&As for Anti-Von Willebrand Factor/VWF Antibody Picoband™

Question

We are currently using anti-Von Willebrand Factor/VWF antibody PB9062 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

The anti-Von Willebrand Factor/VWF antibody (PB9062) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-20

Question

Is there a BSA free version of anti-Von Willebrand Factor/VWF antibody PB9062 available?

V. Lewis

Verified customer

Asked: 2020-02-06

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Von Willebrand Factor/VWF antibody PB9062 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-06

Question

Our team were content with the WB result of your anti-Von Willebrand Factor/VWF antibody. However we have been able to see positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is VWF supposed to be expressed?

V. Evans

Verified customer

Asked: 2019-10-15

Answer

Based on literature, liver does express VWF. Generally VWF expresses in secreted. Regarding which tissues have VWF expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Plasma, Pubmed ID: 14760718
Platelet, Pubmed ID: 12665801
Umbilical vein endothelial cell, Pubmed ID: 3495266

Boster Scientific Support

Answered: 2019-10-15

Question

I have a question about product PB9062, anti-Von Willebrand Factor/VWF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9062 anti-Von Willebrand Factor/VWF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-25

Question

We ordered your anti-Von Willebrand Factor/VWF antibody for IHC-P on plasma a few months ago. I am using human, and I plan to use the antibody for Flow Cytometry next. I was wanting to use examining plasma as well as lung in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

I took a look at the website and datasheets of our anti-Von Willebrand Factor/VWF antibody and it seems that PB9062 has been validated on human in both IHC-P and Flow Cytometry. Thus PB9062 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-08-23

Question

I see that the anti-Von Willebrand Factor/VWF antibody PB9062 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-23

Question

Is a blocking peptide available for product anti-Von Willebrand Factor/VWF antibody (PB9062)?

Verified Customer

Verified customer

Asked: 2019-06-13

Answer

We do provide the blocking peptide for product anti-Von Willebrand Factor/VWF antibody (PB9062). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-13

Question

Would anti-Von Willebrand Factor/VWF antibody PB9062 work for IHC-P with liver?

Verified Customer

Verified customer

Asked: 2019-03-21

Answer

According to the expression profile of liver, VWF is highly expressed in liver. So, it is likely that anti-Von Willebrand Factor/VWF antibody PB9062 will work for IHC-P with liver.

Boster Scientific Support

Answered: 2019-03-21

Question

My question regards to test anti-Von Willebrand Factor/VWF antibody PB9062 on human liver for research purposes, then I may be interested in using anti-Von Willebrand Factor/VWF antibody PB9062 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-05-23

Answer

The products we sell, including anti-Von Willebrand Factor/VWF antibody PB9062, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-05-23

Question

I was wanting to use using your anti-Von Willebrand Factor/VWF antibody for gp1b-ix-v activation signalling studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-05-04

Answer

I appreciate your inquiry. This PB9062 anti-Von Willebrand Factor/VWF antibody is tested on human placenta tissue, lung cancer tissue, ht1080 whole cell lysate, a431 cells. It is guaranteed to work for Flow Cytometry, IHC-P, IHC-F, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-05-04

Question

I was wanting to use your anti-Von Willebrand Factor/VWF antibody for IHC-P for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2017-09-11

Answer

You can see on the product datasheet, PB9062 anti-Von Willebrand Factor/VWF antibody has been validated for Flow Cytometry, IHC-P, IHC-F, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-11

Question

We have been able to see staining in human plasma. Do you have any suggestions? Is anti-Von Willebrand Factor/VWF antibody supposed to stain plasma positively?

J. Moore

Verified customer

Asked: 2016-08-02

Answer

Based on literature plasma does express VWF. Based on Uniprot.org, VWF is expressed in lung, umbilical vein endothelial cell, platelet, plasma, liver, among other tissues. Regarding which tissues have VWF expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Lung, Pubmed ID: 15489334
Plasma, Pubmed ID: 14760718
Platelet, Pubmed ID: 12665801
Umbilical vein endothelial cell, Pubmed ID: 3495266

Boster Scientific Support

Answered: 2016-08-02

Question

Is this PB9062 anti-Von Willebrand Factor/VWF antibody reactive to the isotypes of VWF?

P. Singh

Verified customer

Asked: 2016-04-29

Answer

The immunogen of PB9062 anti-Von Willebrand Factor/VWF antibody is E.coli-derived human VWF recombinant protein (Position: R2535-K2813). Human VWF shares 79% amino acid (aa) sequence identity with mouse VWF. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-04-29

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Von Willebrand Factor/VWF antibody PB9062. Let me know if you need anything else.

D. Singh

Verified customer

Asked: 2014-06-18

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-06-18

Question

See attached the WB image, lot number and protocol we used for liver using anti-Von Willebrand Factor/VWF antibody PB9062. Please let me know if you require anything else.

E. Li

Verified customer

Asked: 2013-10-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-10-22

Question

Will PB9062 anti-Von Willebrand Factor/VWF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

G. Wu

Verified customer

Asked: 2013-08-27

Answer

As indicated on the product datasheet, PB9062 anti-Von Willebrand Factor/VWF antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-08-27

Order DetailsPrice
PB9062

100μg

$370
PB9062-10ug

10μg sample (liquid)

$99
PB9062-Biotin

100 μg Biotin conjugated

$570
PB9062-Cy3

100 μg Cy3 conjugated

$570
PB9062-Dylight488

100 μg Dylight488 conjugated

$570
PB9062-Dylight550

100 μg Dylight550 conjugated

$570
PB9062-Dylight594

100 μg Dylight594 conjugated

$570
PB9062-FITC

100 μg FITC conjugated

$570
PB9062-HRP

100 μg HRP conjugated

$570
PB9062-APC

100 μg APC conjugated

$670
PB9062-PE

100 μg PE conjugated

$670
PB9062-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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