Product Info Summary
SKU: | A04480 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-WISP2 Antibody
SKU/Catalog Number
A04480
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-WISP2 Antibody (Catalog # A04480). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-WISP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04480)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Clone Number
3F8
Isotype
IgG
Immunogen
Peptide sequence around aa.242~246(G-R-S-P-Q) derived from Human WISP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04480 is reactive to CCN5 in Human
Applications
A04480 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
26.825kDa
Background of CCN5
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
Pennica D., Swanson T.A., Welsh J.W. J.Proc. Natl. Acad. Sci. U.S.A. 95:14717-14722(1998)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 27kd
Western blotting: 1:500~1:1000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of WISP2 using anti-WISP2 antibody (A04480).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WISP2 antigen affinity purified polyclonal antibody (Catalog # A04480) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for WISP2.
Protein Target Info & Infographic
Gene/Protein Information For CCN5 (Source: Uniprot.org, NCBI)
Gene Name
CCN5
Full Name
CCN family member 5
Weight
26.825kDa
Superfamily
CCN family
Alternative Names
CCN family member 5 CCN5 CT58, CTGF-L, WISP2 cellular communication network factor 5 CCN family member 5|WNT1 inducible signaling pathway protein 2|connective tissue growth factor-like protein|connective tissue growth factor-related protein 58
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCN5, check out the CCN5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCN5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WISP2 Antibody (A04480)
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No publications found for A04480
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-WISP2 Antibody
Question
We are currently using anti-WISP2 antibody A04480 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
The anti-WISP2 antibody (A04480) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-06