Anti-Wnt2 Antibody Picoband™

Wnt-2 antibody

Boster Bio Anti-Wnt2 Antibody Picoband™ catalog # PB9461. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PB9461
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Wnt2 Antibody Picoband™

View all Wnt-2 Antibodies

SKU/Catalog Number

PB9461

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Wnt2 Antibody Picoband™ catalog # PB9461. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Wnt2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9461)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt2, different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9461 is reactive to WNT2 in Human

Applications

PB9461 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

40 kDa

Calculated molecular weight

40.418kDa

Background of Wnt-2

WNT2, also known as Wingless-type MMTV integration site family, member 2, is a human gene. This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For WNT2 (Source: Uniprot.org, NCBI)

Gene Name

WNT2

Full Name

Protein Wnt-2

Weight

40.418kDa

Superfamily

Wnt family

Alternative Names

INT1L1; INT1L1secreted growth factor; Int-1-like protein 1; Int-1-related protein; IRP; IRPprotein Wnt-2; wingless-type MMTV integration site family member 2; Wnt2; Wnt-2 WNT2 INT1L1, IRP Wnt family member 2 protein Wnt-2|Int-1-related protein|epididymis secretory sperm binding protein|int-1-like protein 1|secreted growth factor|wingless-type MMTV integration site family member 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on WNT2, check out the WNT2 Infographic

WNT2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for WNT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Wnt2 Antibody Picoband™

Question

Would PB9461 anti-Wnt2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-04-26

Answer

You can see on the product datasheet, PB9461 anti-Wnt2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-04-26

Question

Do you have a BSA free version of anti-Wnt2 antibody PB9461 available?

Verified Customer

Verified customer

Asked: 2018-12-21

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Wnt2 antibody PB9461 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-12-21

Question

We are currently using anti-Wnt2 antibody PB9461 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?

G. Edwards

Verified customer

Asked: 2017-11-22

Answer

The anti-Wnt2 antibody (PB9461) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-22

Order DetailsPrice
PB9461

100μg

$370
PB9461-10ug

10μg sample (liquid)

$99
PB9461-Biotin

100 μg Biotin conjugated

$570
PB9461-Cy3

100 μg Cy3 conjugated

$570
PB9461-Dylight488

100 μg Dylight488 conjugated

$570
PB9461-Dylight550

100 μg Dylight550 conjugated

$570
PB9461-Dylight594

100 μg Dylight594 conjugated

$570
PB9461-FITC

100 μg FITC conjugated

$570
PB9461-HRP

100 μg HRP conjugated

$570
PB9461-APC

100 μg APC conjugated

$670
PB9461-PE

100 μg PE conjugated

$670
PB9461-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9461
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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