Product Info Summary
SKU: | PB9619 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Wnt3 Antibody Picoband™
SKU/Catalog Number
PB9619
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Wnt3 Antibody Picoband™ catalog # PB9619. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Wnt3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9619)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Wnt3, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9619 is reactive to WNT3 in Human
Applications
PB9619 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
39.645kDa
Background of WNT3
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of WNT3 using anti-WNT3 antibody (PB9619).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Human Placenta Tissue Lysate at 50ug,
Lane 2: HEPG2 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT3 antigen affinity purified polyclonal antibody (Catalog # PB9619) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT3 at approximately 45 kDa. The expected band size for WNT3 is at 45 kDa.
Protein Target Info & Infographic
Gene/Protein Information For WNT3 (Source: Uniprot.org, NCBI)
Gene Name
WNT3
Full Name
Proto-oncogene Wnt-3
Weight
39.645kDa
Superfamily
Wnt family
Alternative Names
INT4Proto-oncogene Int-4 homolog; MGC131950; MGC138321; MGC138323; proto-oncogene Wnt-3; wingless-type MMTV integration site family, member 3; WNT-3 proto-oncogene protein WNT3 INT4, TETAMS Wnt family member 3 proto-oncogene Wnt-3|WNT-3 proto-oncogene protein|proto-oncogene Int-4 homolog|wingless-type MMTV integration site family, member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WNT3, check out the WNT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WNT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Wnt3 Antibody Picoband™ (PB9619)
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No publications found for PB9619
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Customer Q&As
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1 Customer Q&As for Anti-Wnt3 Antibody Picoband™
Question
We are currently using anti-Wnt3 antibody PB9619 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The anti-Wnt3 antibody (PB9619) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-04