Antibody Diluent for Western Blotting

antibody

Boster’s Antibody Diluent for Western Blotting consists of BSA, detergent and antibody stabilizer. This buffer helps to reduce non-specific binding of primary antibody in WB and effectively increase storage time of the primary antibody working solution. Cited in 7 publication(s).

Product Info Summary

SKU: AR1017
Size: 500 mL (50 assays)

Overview

Product Name Antibody Diluent for Western Blotting
SKU/Catalog Number AR1017
Pack Size 500 mL (50 assays)
Form Liquid
Type Ready to use
Contents BSA, detergent and antibody stabilizer
Storage Store at 4°C for one year. Avoid multiple freeze-thaw cycles.
Application This diluent is used used to dilute the primary or secondary antibody when performing Western Blot.
*Our Boster Guarantee covers the use of this product in the above tested applications.
Description Boster’s Antibody Diluent for Western Blotting consists of BSA, detergent and antibody stabilizer. This buffer helps to reduce non-specific binding of primary antibody in WB and effectively increase storage time of the primary antibody working solution.
Cite This Product Antibody Diluent for Western Blotting (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1017)

Assay Principle

Boster’s Antibody Diluent for Western Blotting consists of BSA, detergent and antibody stabilizer. This buffer helps to reduce non-specific binding of the primary antibody in WB and effectively increase storage time of diluted primary antibody.

Usage

Refer to the instructions for use of the primary antibody and the expression level of the target protein in the sample, and dilute the primary antibody in an appropriate ratio. Diluted primary antibody can be used directly in Western. Unused diluted primary antibody can be stored at 4°C for future use.

Note

1. In order to enable the antibody to be used repeatedly, it is recommended to perform the binding reaction between the primary antibody and the protein membrane at 4°C to slow down the decay rate of the primary antibody.

2. Please do not use the Antibody Diluent if there is any precipitation or bacterial contamination.

Validation Images & Assay Conditions

AR1017 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Role of CYP4F2 as a novel biomarker regulating malignant phenotypes of liver cancer cells via the Nrf2 signaling axis

Evodiamine alleviates severe pneumonia induced by methicillin-susceptible Staphylococcus aureus following cytomegalovirus reactivation through suppressing NF-κB and MAPKs

miR‑378a‑3p exerts tumor suppressive function on the tumorigenesis of esophageal squamous cell carcinoma by targeting Rab10

Facile Surface Modification Method for Synergistically Enhancing the Biocompatibility and Bioactivity of Poly(ether ether ketone) That Induced Osteodifferentiation

IDO1 as a new immune biomarker for diabetic nephropathy and its correlation with immune cell infiltration

Yu K,Li D,Xu F,Guo H,Feng F,Ding Y,Wan X,Sun N,Zhang Y,Fan J,Liu L,Yang H,Yang X.IDO1 as a new immune biomarker for diabetic nephropathy and its correlation with immune cell infiltration.Int Immunopharmacol.2021 Feb 10;94:107446.doi:10.1016/j.intimp.2021.
Species: Mouse
AR1017 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:NA

Lin C,Zhang Y,Wang J,Sui A,Xiu L,Zhu X.The study of effect and mechanism of 630-nm laser on human lung adenocarcinoma cell xenograft model in nude mice mediated by hematoporphyrin derivatives.Lasers Med Sci.2020 Jul;35(5):1085-1094.doi:10.1007/s10103-019-
Species: Mouse
AR1017 usage in article: APP:WB, SAMPLE:TUMOR TISSUE, DILUTION:NA

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