Show Order Info
SKU AR1109
Pack size 10 mL (for 1000 assays)
Applications Mounting tissue or cell slides for immunofluorescence
Mounting slides for chromosome fluorescence
Mounting slides for nucleic acid fluorescence in hybridization in situ

Overview

Product Name Antifade Mounting Medium
SKU/Catalog Number AR1109
Pack Size 10 mL (for 1000 assays)
Storage Store at 4˚C, protected from light. It is stable for 6 months.
Application Mounting tissue or cell slides for immunofluorescence
Mounting slides for chromosome fluorescence
Mounting slides for nucleic acid fluorescence in hybridization in situ
*Our Boster Guarantee covers the use of this product in the above tested applications.
Equivalent Millipore Sigma (Product No. 10981), Cell Signaling Technology (Product No. 9071S)
Description Boster’s Antifade Mounting Medium is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides.
Cite This Product Antifade Mounting Medium (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1109)

Assay Principle

Antifade Mounting Medium is a glycerol-based liquid mountant applied directly to fluorescently labeled cells or tissue samples on microscope slides. Mounted slides can be stored at 4˚C or -20˚C for 2-3 weeks, protected from light. You can use Antifade Mounting Medium with almost any fluorescent dye or fluorescent protein to achieve the brightest signal and lowest background, for bright, high definition fluorescent images. It protects fluorescent dyes or fluorescent proteins against photobleaching. It is proven to have best-in-class performance with a wide array of fluorescent dyes, including FITC, Cy2, Cy3, Rhodamine, Texas Red, Cy5, Cy7, DAPI, AMCA, R6G, Hoechst 33258.

Assay Protocol

1. Equilibrate the Antifade Mounting Medium to room temperature before use.

2. Absorb liquid on the slice, and dry in the air.

3. Add 20 μL of Antifade Mounting Medium onto the slide, or add a proper volume into cell seed wells.

4. Cover the slide carefully avoiding air bubbles.

5. Remove excess liquid from coverslip.

6. Clean coverslip and image tissues and cells.

Antifade Mounting Medium Images

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Antifade Mounting Medium
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Publications

In vitro evaluation of aspirin-induced HspB1 against heat stress damage in chicken myocardial cells
The Kinase Complex mTOR Complex 2 Promotes the Follicular Migration and Functional Maturation of Differentiated Follicular Helper CD4+ T Cells During Viral Infection
Long non-coding RNA BC168687 small interfering RNA reduces high glucose and high free fatty acid-induced expression of P2X7 receptors in satellite glial cells
Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum
Electroacupuncture at Fengchi (GB20) inhibits calcitonin gene-related peptide expression in the trigeminovascular system of a rat model of migraine
Effect of intravitreal injection of ranibizumab on retinal ganglion cells and microvessels in the early stage of diabetic retinopathy in rats with streptozotocin-induced diabetes
Inhibition of lymphangiogenesis in vitro and in vivo by the multikinase inhibitor nintedanib
Astaxanthin protects against early burn-wound progression in rats by attenuating oxidative stress-induced inflammation and mitochondria-related apoptosis
Cellular Localization and Regulation of Expression of the PLET1 Gene in Porcine Placenta
Effect of electroacupuncture pretreatment at GB20 on behaviour and the descending pain modulatory system in a rat model of migraine
Brilliant blue G attenuates lipopolysaccharide-mediated microglial activation and inflammation
A novel decoy receptor fusion protein for FGF-2 potently inhibits tumour growth
Identification of Key Genes in the Response to?Salmonella enterica Enteritidis,?Salmonella enterica Pullorum, and Poly(I:C) in Chicken Spleen and Caecum
The expression and distribution of aquaporin 3 in mouse embryos before and after vitrification
A comparing study of quantitative staining techniques for retinal neovascularization in a mouse model of oxygen-induced retinopathy
Li B, Wu H, Li N, Su J, Jia R, Jiang J, Feng Y, Xiao L. Front Microbiol. 2017 Aug 31;8:1647. doi: 10.3389/fmicb.2017.01647. eCollection 2017. Preliminary Characterization of MEDLE-2, a Protein Potentially Involved in the Invasion of Cryptosporidium parvum
Xiao A, Zhou Q, Shao Y, Zhong HF. Exp Ther Med. 2017 Jun;13(6):3360-3368. doi: 10.3892/etm.2017.4431. Epub 2017 May 5. Effect of intravitreal injection of ranibizumab on retinal ganglion cells and microvessels in the early stage of diabetic retinopathy in rats with streptozotocin-induced diabetes
Fang Q, Guo S, Zhou H, Han R, Wu P, Han C. Sci Rep. 2017 Jan 27;7:41440. doi: 10.1038/srep41440. Astaxanthin protects against early burn-wound progression in rats by attenuating oxidative stress-induced inflammation and mitochondria-related apoptosis
Liu CL, Deng ZY, Du ER, Xu CS. Mol Med Rep. 2018 Apr;17(4):5851-5859. doi: 10.3892/mmr.2018.8601. Epub 2018 Feb 13. Long non-coding RNA BC168687 small interfering RNA reduces high glucose and high free fatty acid-induced expression of P2X7 receptors in satellite glial cells
Su, N., Gao, P.L., Wang, K., Wang, J.Y., Zhong, Y., & Luo, Y. (2017). Fibrous scaffolds potentiate the paracrine function of mesenchymal stem cells: A new dimension in cell-material interaction. Biomaterials, 141, 74-85. Advance online publication. doi: 10.1016/j.biomaterials.2017.06.028
Zhao, L.P., Liu, L., Pei, P., Qu, Z.Y., Zhu, Y.P., & Wang, L.P. (2017). Electroacupuncture at Fengchi (GB20) inhibits calcitonin gene-related peptide expression in the trigeminovascular system of a rat model of migraine. Neural Regeneration Research, 12(5), 804-811. doi: 10.4103/1673-5374.206652
Wang, X., Wang, K., Zhang, W., Qiang, M., & Luo, Y. (2017). A bilaminated decellularized scaffold for islet transplantation: Structure, properties and functions in diabetic mice. Biomaterials, 138, 80-90. doi: 10.1016/j.biomaterials.2017.05.033
Lin T, Gong L. Drug Des Devel Ther. 2017 Apr 5;11:1147-1158. doi: 10.2147/DDDT.S130297. eCollection 2017. Inhibition of lymphangiogenesis in vitro and in vivo by the multikinase inhibitor nintedanib.

Customer Q&As

Q: Does AR1109 contain propidium iodide?
A: AR1109 does not contain propidium iodide.