|Product Name||IHC enzyme antigen retrieval reagent|
|Synonyms||Compound Digest Solution, Enzymic digestion solution, Proteolytic enzyme solution, Proteolytic antigen retrieval solution, Proteolytic antigen retrieval reagent, Enzymatic antigen retrieval reagent, Tissue digest solution|
|Description||IHC enzyme antigen retrieval reagent is a ready to use proteolytic enzyme solution intended to be used as a pretreatment reagent in IHC for enzymatic digestion of tissues employed in proteolytic induced epitope retrieval (PIER) techniques applied upon paraffin-embedded formalin-fixed (PEFF) tissue sections on slides following deparaffinization and prior to antibody staining.|
|Application||IHC, PIER/EIER, PEFF tissues|
|Pack Size||50ml||Type||Buffers and Mixes||Research Area||Molecular Biology, Cell Biology, Hystology|
|Physical State||Slightly viscous liquid|
|Pack Size||50 ml|
|Volume of final solution||50 ml|
|Storage||Store at 4°C for one year|
|Restrictions||For Research Use only|
Usage and Handling
The use of enzyme digestion method may destroy some epitopes and tissue morphology. In these cases HIER methods for antigen retrieval should be used.
When using the enzymatic digestion reagents it is important to not over or under digest. Preliminary experiments should be conducted to determine the optimum incubation time, temperature, and enzyme concentration.
In general, enzymatic methods are shown to improve IHC staining results less than HIER and are much less reproducible. Their use is primarily recommended for difficult-to-retrieve epitopes when HIER fails to produce satisfactory results.
IHC Enzyme Antigen Retrieval Reagent is for scientific laboratory research purposes and is not for diagnostic, therapeutic, prophylactic or in vivo applications.
The IHC Enzyme Antigen Retrieval Reagent contains multiple proteinases that catalyze proteolysis in tissue samples, breaking the proteins into smaller peptide fractions and amino acids. Enzymatic digestion is commonly used in IHC in the PIER method for antigen retrieval to overcome the loss of immunoreactivity in epitopes caused by the covalent cross-links (methylene bridges) that are formed in tissues during formalin fixation. Proteolysis serves to improve the accessibility of antibodies to target antigens by two main mechanisms: by increasing the penetration of immunoreagents into tissue structures as a result of both tissue digestion and relaxation of the protein structure rigidity, and by restoring the methylene-bridged epitopes. Since proteases exhibit different cleavage site specificity, the combination of multiple proteinases provides a broader spectrum of proteins digestion in a tissue sample. Thus various types of antigenic targets (e.g. protein, glycoprotein, or non-protein, inter- or intracellular antigens) can benefit from this cocktail of digestion enzymes.
Antigen Retrieval Buffer (Enzymatic Digestion) For IHC Images
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50 mL per pack, Solution (liquid).