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SKU AR0022
Pack size 50ml
Form Solution (liquid)
Applications IHC
PIER/EIER
PEFF tissues
Price: $55.00
Quantity:

Overview

Product Name IHC enzyme antigen retrieval reagent
Synonyms Compound Digest Solution, Enzymic digestion solution, Proteolytic enzyme solution, Proteolytic antigen retrieval solution, Proteolytic antigen retrieval reagent, Enzymatic antigen retrieval reagent, Tissue digest solution
Description IHC enzyme antigen retrieval reagent is a ready to use proteolytic enzyme solution intended to be used as a pretreatment reagent in IHC for enzymatic digestion of tissues employed in proteolytic induced epitope retrieval (PIER) techniques applied upon paraffin-embedded formalin-fixed (PEFF) tissue sections on slides following deparaffinization and prior to antibody staining.
Application IHC, PIER/EIER, PEFF tissues
Pack Size 50ml
Type Buffers and Mixes
Research Area Molecular Biology, Cell Biology, Hystology

Properties

Content Multiple proteinases
Composition Proteolytic Enzymes
Form Supplied Solution
Physical State Slightly viscous liquid
Pack Size 50 ml
Volume of final solution 50 ml
Enzymatic activity Protease
Storage Store at 4°C for one year
Restrictions For Research Use only

Usage and Handling

The use of enzyme digestion method may destroy some epitopes and tissue morphology. In these cases HIER methods for antigen retrieval should be used.

When using the enzymatic digestion reagents it is important to not over or under digest. Preliminary experiments should be conducted to determine the optimum incubation time, temperature, and enzyme concentration.

In general, enzymatic methods are shown to improve IHC staining results less than HIER and are much less reproducible. Their use is primarily recommended for difficult-to-retrieve epitopes when HIER fails to produce satisfactory results.

IHC Enzyme Antigen Retrieval Reagent is for scientific laboratory research purposes and is not for diagnostic, therapeutic, prophylactic or in vivo applications.

Background

The IHC Enzyme Antigen Retrieval Reagent contains multiple proteinases that catalyze proteolysis in tissue samples, breaking the proteins into smaller peptide fractions and amino acids. Enzymatic digestion is commonly used in IHC in the PIER method for antigen retrieval to overcome the loss of immunoreactivity in epitopes caused by the covalent cross-links (methylene bridges) that are formed in tissues during formalin fixation. Proteolysis serves to improve the accessibility of antibodies to target antigens by two main mechanisms: by increasing the penetration of immunoreagents into tissue structures as a result of both tissue digestion and relaxation of the protein structure rigidity, and by restoring the methylene-bridged epitopes. Since proteases exhibit different cleavage site specificity, the combination of multiple proteinases provides a broader spectrum of proteins digestion in a tissue sample. Thus various types of antigenic targets (e.g. protein, glycoprotein, or non-protein, inter- or intracellular antigens) can benefit from this cocktail of digestion enzymes.

Antigen Retrieval Buffer (Enzymatic Digestion) For IHC Images

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Antigen Retrieval Buffer (Enzymatic Digestion) For IHC
Immunohistochemistry - IHC enzyme antigen retrieval reagent (AR0022)
50 mL per pack, Solution (liquid).
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Publications

Wang, J., Zhang, S., Wu, J., Lu, Z., Yang, J., Wu, H.,…, & Cao, T. (2017). Clinical significance and prognostic value of SOX7 expression in liver and pancreatic carcinoma. Molecular Medicine Reports, 16(1), 499-506. doi: 10.3892/mmr.2017.6660