ARHGEF2 (Phospho-Ser885) Colorimetric Cell-Based ELISA Kit

GEF-H1 ELISA kit, phospho-specific, cell-based

The ARHGEF2 (Phospho-Ser885) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ARHGEF2 (Phospho-Ser885) protein expression profile in cells. The kit can be used for measuring the relative amounts of ARHGEF2 (Phospho-Ser885) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ARHGEF2 (Phospho-Ser885).

Product Info Summary

SKU: EKC2338
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

ARHGEF2 (Phospho-Ser885) Colorimetric Cell-Based ELISA Kit

See all GEF-H1 products

SKU/Catalog Number

EKC2338

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The ARHGEF2 (Phospho-Ser885) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ARHGEF2 (Phospho-Ser885) protein expression profile in cells. The kit can be used for measuring the relative amounts of ARHGEF2 (Phospho-Ser885) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ARHGEF2 (Phospho-Ser885).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

ARHGEF2 (Phospho-Ser885) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2338)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2338 is reactive to ARHGEF2 in Human, Mouse, Rat samples

Application Guarantee

EKC2338 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-ARHGEF2 (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-ARHGEF2 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For ARHGEF2 (Source: Uniprot.Org, NCBI)

Gene Name

ARHGEF2

Full Name

Rho guanine nucleotide exchange factor 2

Weight

111.543kDa

Alternative Names

GEF; GEFH1; GEF-H1DKFZp547P1516; Guanine nucleotide exchange factor H1; KIAA0651DKFZp547L106; LFP40FLJ45440; Microtubule-regulated Rho-GEF; P40; Proliferating cell nucleolar antigen p40; rho guanine nucleotide exchange factor 2; Rho/Rac guanine nucleotide exchange factor (GEF) 2; Rho/Rac guanine nucleotide exchange factor 2 ARHGEF2 GEF, GEF-H1, GEFH1, LFP40, Lfc, NEDMHM, P40 Rho/Rac guanine nucleotide exchange factor 2 rho guanine nucleotide exchange factor 2|Rho/Rac guanine nucleotide exchange factor (GEF) 2|guanine nucleotide exchange factor H1|microtubule-regulated Rho-GEF|proliferating cell nucleolar antigen p40

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ARHGEF2, check out the ARHGEF2 Infographic

ARHGEF2 infographic

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In this infographic you will see the following information for ARHGEF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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