Product Info Summary
SKU: | EKC2338 |
---|---|
Size: | 1 kit, containing two 96-well plates and all necessary reagents |
Reactive Species: | Human, Mouse, Rat |
Application: | ELISA |
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Product info
Product Name
ARHGEF2 (Phospho-Ser885) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2338
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The ARHGEF2 (Phospho-Ser885) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ARHGEF2 (Phospho-Ser885) protein expression profile in cells. The kit can be used for measuring the relative amounts of ARHGEF2 (Phospho-Ser885) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ARHGEF2 (Phospho-Ser885).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
ARHGEF2 (Phospho-Ser885) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2338)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2338 is reactive to ARHGEF2 in Human, Mouse, Rat samples
Application Guarantee
EKC2338 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent | Quantity | Container |
---|---|---|
96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
10x TBS | 24 ml (10x) | Clear |
Quenching Buffer | 24 ml (1x) | Clear |
Blocking Buffer | 50 ml (1x) | Clear |
15x Wash Buffer | 50 ml (15x) | Clear |
100x Anti-ARHGEF2 (Phospho-specific) Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
100x Anti-ARHGEF2 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
Primary Antibody Diluent | 12 ml (1x) | Clear |
Ready-to-Use Substrate | 12 ml (1x) | Brown |
Stop Solution | 12 ml (1x) | Clear |
Crystal Violet Solution | 12ml (1x) | Glass |
SDS Solution | 24 ml (1x) | Clear |
Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HeLa cells treated with TSA 400nM 24H and Jurkat cells treated with forskolin 40nM 30'
Protein Target Info & Infographic
Gene/Protein Information For ARHGEF2 (Source: Uniprot.Org, NCBI)
Gene Name
ARHGEF2
Full Name
Rho guanine nucleotide exchange factor 2
Weight
111.543kDa
Alternative Names
GEF; GEFH1; GEF-H1DKFZp547P1516; Guanine nucleotide exchange factor H1; KIAA0651DKFZp547L106; LFP40FLJ45440; Microtubule-regulated Rho-GEF; P40; Proliferating cell nucleolar antigen p40; rho guanine nucleotide exchange factor 2; Rho/Rac guanine nucleotide exchange factor (GEF) 2; Rho/Rac guanine nucleotide exchange factor 2 ARHGEF2 GEF, GEF-H1, GEFH1, LFP40, Lfc, NEDMHM, P40 Rho/Rac guanine nucleotide exchange factor 2 rho guanine nucleotide exchange factor 2|Rho/Rac guanine nucleotide exchange factor (GEF) 2|guanine nucleotide exchange factor H1|microtubule-regulated Rho-GEF|proliferating cell nucleolar antigen p40
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ARHGEF2, check out the ARHGEF2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ARHGEF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ARHGEF2 (Phospho-Ser885) Colorimetric Cell-Based ELISA Kit (EKC2338)
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