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SKU AR0146
Product nameBCA Protein Assay Kit(for 20-2000 μg/ml protein)
Pack size 1 kit
Applications Western blotting, protein expression assays, protein profiling and characterization, protein quantitation assays

List of Components

DescriptionQuantityVolumeContents
BCA Reagent A1100mLcontaining sodium carbonate, sodium bicarbonate, bicinchoninic acid and sodium tartrate in 0.1M sodium hydroxide
BCA Reagent B15mLcontaining 4% cupric sulfate
Albumin (BSA) Standards120mL (2mg/mL)containing bovine serum albumin (BSA) at 2mg/mL in 0.9% saline and 0.05% sodium azide

Overview

Form Supplied BCA Reagent A and B: ready-to-use 1X solutions
BSA Standard: stock solution for preparing a series of standard protein dilutions
Assays per kit Tube procedure: 50 assays
Microplate procedure: 500 assays
Storage Upon receipt store at room temperature.
It is stable at room temperature for one year.
Note If either Reagent A or Reagent B precipitates upon shipping in cold weather or during long-term storage, dissolve precipitates by gently warming and stirring solution. Discard any kit reagent that shows discoloration or evidence of microbial contamination.
Assay Range 20 - 2000μg/mL
Equivalent Thermofisher (Product No. 23227), Assay Range: 20 - 2000μg/mL
Millipore Sigma (Product No. BCA1), Assay Range: 200 - 1000μg/mL
Compatibility with reagents Compatible with typical concentrations of most ionic and nonionic detergents
Incompatible with chelating agents, strong acids or bases, and reducing agents: interfere with the reduction and chelating reactions of the assay mechanism
Applications Western blotting, protein expression assays, protein profiling and characterization, protein quantitation assays
*Our Boster Guarantee covers the use of this product in the above tested applications.
Physical State of A+B Reagent mixture Light blue liquid
Reagent Color/Absorbance Blue / A562 nm
Activity Active in alkaline medium
Description BCA Protein Assay Kit is a ready-to-use detergent-compatible Western blot related total protein analysis reagent used for the quick determination of total protein concentration by measuring absorbance at 562 nm and comparing to a protein standard absorption vs. concentration curve, according to Smith. The protein quantification process can be finished in 45 minutes.
Cite This Product BCA Protein Assay Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0146)

Assay Information

Assay Type Protein Quantitative Measurement
Sample Type Serum, Plasma, Cell culture extracts, Tissue Extracts
Technique Solution-based detection of spectral absorbance at 562 nm; Smith Protein assay
Detection Method Colorimetric/Spectroscopic
Equipment needed Test tubes or miscroplates; Spectrophotometer; Microplate Reader
Assay Purpose Measure protein concentration in solution
Incubation Requirements 30 min at 37°C
To be used with Lysates or homogenates, prepared with non-reducing lysis and extraction buffers: Mammal Tissue Protein Extraction Reagent, Mammal Cell Protein Extraction Reagent, RIPA Lysis Buffer, Mitochondria Extraction Reagent, Membrane Protein Extraction Kit, Cytoplasmic and Nuclear Extraction Kit, Bacterial Cell Protein Extraction Reagent

Assay Principle

The BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantization of total protein. This method combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) using a unique reagent containing bicinchoninic acid. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range (20-2,000 μg/ml).
The BCA method is not a true end-point method; that is, the final color continues to develop. However, following incubation, the rate of continued color development is sufficiently slow to allow large numbers of samples to be assayed together. The macromolecular structure of protein, the number of peptide bonds and the presence of four particular amino acids (cysteine, cystine, tryptophan and tyrosine) are reported to be responsible for color formation with BCA. Studies with di-, tri- and tetrapeptides suggest that the extent of color formation caused by more than the mere sum of individual color producing functional groups.
Accordingly, protein concentrations generally are determined and reported with reference to standards of a common protein such as bovine serum albumin (BSA). A series of dilutions of known concentration are prepared from the protein and assayed alongside the unknown before the concentration of each unknown is determined based on the standard curve. If precise quantization of an unknown protein is required, it is advisable to select a protein standard that is similar in quality to the unknown; for example, a bovine gamma globulin (BGG) standard may be used when assaying immunoglobulin samples. Two assay procedures are presented. Of these, the Test Tube Procedure requires a larger volume (0.1 ml) of protein sample; however, because it uses a sample to working reagent ratio of 1:20 (v/v), the effect of interfering substances is minimized.

Additional Materials Required

• 37°C water bath or 37°C incubator

• Microplate reader and spectrophotometer capable of accurately measuring absorbance in the region of 540-595nm (562nm is the optimal wavelength)

Important product information

1. If this kit is received or stored cold, a precipitate may form in Reagent A or Reagent B. To dissolve the precipitate, warm the solution slowly at 37°C while mixing or microwave for a few seconds. Discard the kit if it is contaminated by bacteria

2. If interference caused by reducing substances or metal-chelating substances contained in the sample remains, Bradford Assay Kit is recommended.

3. It is recommended that the standard of different concentrations and samples be assayed in duplicate. Standard curve should be plotted for each assay.

4. Newly formed green turbidity will disappear after mixing Reagent A and Reagent B thoroughly. It will not affect the performance.

5. Assayed sample amount will be reduced while using spectrophotometer to detect protein concentration. When using 37°C incubator, prevent the influence of water evaporation.

6. Several ways for eliminating or minimizing the effects of interfering substances:
• Remove interfering substances by dialysis or gel filtration.
• Dilute the sample until substances no longer interfere.
• Since detergents of high concentrations also affect the results, precipitate the proteins in the sample with trichloroacetic acid (TCA).

7. Avoid using substances including reducing substances, chelating agents, strong acid and alkali since they interfere with protein estimation even at very low concentration.

BCA Protein Assay Kit Images

Click the images to enlarge.

BCA Protein Assay Kit
BCA Protein Assay Kit
Standard Curve of Net Absorbance versus protein sample concentration (Incubate at 37°C for 30 minutes).
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Publications

Sequencing and Expression Characterization of Antifreeze Protein Maxi-Like in Apis cerana cerana
Alteration of Histone Acetylation Pattern during Long-Term Serum-Free Culture Conditions of Human Fetal Placental Mesenchymal Stem Cells
Reduced ?2GPI Inhibiting Glomerular Mesangial Cells VEGF-NO Axis Uncoupling Induced by High Glucose
Effect of dexmedetomidine, midazolam, and propofol on lipopolysaccharide-stimulated dendritic cells
MicroRNA-146a suppresses rheumatoid arthritis fibroblast-like synoviocytes proliferation and inflammatory responses by inhibiting the TLR4/NF-kB signaling
Downregulation of RIP140 in triple-negative breast cancer inhibits the growth and proliferation of cancer cells
Expression and prognostic value of CLIC1 in epithelial ovarian cancer
Panax ginseng extract attenuates neuronal injury and cognitive deficits in rats with vascular dementia induced by chronic cerebral hypoperfusion
miR-488 inhibits cell growth and metastasis in renal cell carcinoma by targeting HMGN5
Artemether attenuates LPS-induced inflammatory bone loss by inhibiting osteoclastogenesis and bone resorption via suppression of MAPK signaling pathway
PTEN gene silencing contributes to airway remodeling and induces airway smooth muscle cell proliferation in mice with allergic asthma
miR-518b Enhances Human Trophoblast Cell Proliferation Through Targeting Rap1b and Activating Ras-MAPK Signal
Yak IGF2 Promotes Fibroblast Proliferation Via Suppression of IGF1R and PI3KCG Expression
Treatment with 20(S)-ginsenoside Rg3 reverses multidrug resistance in A549/DDP xenograft tumors
Tanshinone IIA prevents left ventricular remodelling via the TLR4/MyD88/NF??B signalling pathway in rats with myocardial infarction
Effect of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells
Mdivi-1 pretreatment mitigates isoflurane-induced cognitive deficits in developmental rats
Effect of the Notch signaling pathway on retinal ganglion cells and its neuroprotection in rats with acute ocular hypertension
Long non-coding RNA SPRY4-IT1 promotes the proliferation and invasion of U251 cells through upregulation of SKA2
Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal Stem Cells through Canonical Wnt Signaling Pathway
Folate deficiency and aberrant DNA methylation and expression of FHIT gene were associated with cervical pathogenesis
IL-8 is upregulated in cervical cancer tissues and is associated with the proliferation and migration of HeLa cervical cancer cells
Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1?-induced cartilage catabolic degradation through MAPK/JNK and ERK pathways
Low-dose dexamethasone affects osteoblast viability by inducing autophagy via intracellular ROS
Proteasome Inhibitor Carbobenzoxy-L-Leucyl-L-Leucyl-L-Leucinal (MG132) Enhances Therapeutic Effect of Paclitaxel on Breast Cancer by Inhibiting Nuclear Factor (NF)-?B Signaling
GOLPH3: a novel biomarker that correlates with poor survival and resistance to chemotherapy in breast cancer
Effects of vaspin on pancreatic ? cell secretion via PI3K/Akt and NF-?B signaling pathways
Upregulation of Cdh1 Attenuates Isoflurane-Induced Neuronal Apoptosis and Long-Term Cognitive Impairments in Developing Rats
Overexpression of Cullin7 is associated with hepatocellular carcinoma progression and pathogenesis
Long noncoding RNA CCAT1 functions as a ceRNA to antagonize the effect of miR-410 on the down-regulation of ITPKB in human HCT-116 and HCT-8 cells
Neuroprotective Effect of Ligustilide through Induction of ?-Secretase Processing of Both APP and Klotho in a Mouse Model of Alzheimer?s Disease
Dachaihu decoction ameliorates pancreatic fibrosis by inhibiting macrophage infiltration in chronic pancreatitis
Root-Securing and Brain-Fortifying Liquid Upregulates Caveolin-1 in Cell Model with Alzheimer's Disease through Inhibiting Tau Phosphorylation
Overexpression of microRNA-132 enhances the radiosensitivity of cervical cancer cells by down-regulating Bmi-1
Changes of Foxo3a in PBMCs and its associations with stress hyperglycemia in acute obstructive suppurative cholangitis patients
Citalopram Ameliorates Impairments in Spatial Memory and Synaptic Plasticity in Female 3xTgAD Mice
Resveratrol-based cinnamic ester hybrids: synthesis, characterization, and anti-inflammatory activity
Different effection of p.1125Val>Ala and rs11954856 in APC on Wnt signaling pathway
Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis Partially by Upregulating PXR
Mechanism of apoptosis involved in gastric mucosal lesions in Tibetans with high-altitude polycythemia
Hypoxia-Induced Activation of JAK/STAT3 Signaling Pathway Promotes Trophoblast Cell Viability and Angiogenesis in Preeclampsia
Long non-coding RNA H19 induces hippocampal neuronal apoptosis via Wnt signaling in a streptozotocin-induced rat model of diabetes mellitus
MicroRNA-373 Inhibits Cell Proliferation and Invasion via Targeting BRF2 in Human Non-small Cell Lung Cancer A549 Cell Line
ASIC1a Promotes Acid-Induced Autophagy in Rat Articular Chondrocytes through the AMPK/FoxO3a Pathway
Iguratimod prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator-activated receptor-?
Chikusetsu saponin IVa attenuates isoflurane-induced neurotoxicity and cognitive deficits via SIRT1/ERK1/2 in developmental rats
MicroRNA-145 induces apoptosis of glioma cells by targeting BNIP3 and Notch signaling
Honokiol protects against doxorubicin cardiotoxicity via improving mitochondrial function in mouse hearts
Protective effects of heme oxygenase-1-transduced bone marrow-derived mesenchymal stem cells on reduced-size liver transplantation: Role of autophagy regulated by the ERK/mTOR signaling pathway
miR-487b mitigates chronic heart failure through inhibition of the IL-33/ST2 signaling pathway
Regulated differentiation of WERI-Rb-1 cells into retinal neuron-like cells
Identification of relevant prognostic values of cytokeratin 20 and cytokeratin 7 expressions in lung cancer
MicroRNA-93 inhibits apoptosis and promotes proliferation, invasion and migration of renal cell carcinoma ACHN cells via the TGF-?/Smad signaling pathway by targeting RUNX3
A novel indication of thioredoxin-interacting protein as a tumor suppressor gene in malignant glioma
Soluble Epoxide Hydrolase Inhibition Protected against Angiotensin II-induced Adventitial Remodeling
Effects of RNA interference-mediated gene silencing of VEGF on the ultrafiltration failure in a rat model of peritoneal dialysis
Heme Oxygenase-1, a Key Enzyme for the Cytoprotective Actions of Halophenols by Upregulating Nrf2 Expression via Activating Erk1/2 and PI3K/Akt in EA.hy926 Cells
Transforming growth factor-?1 contributes to oxaliplatin resistance in colorectal cancer via epithelial to mesenchymal transition
Sodium salicylate modulates inflammatory responses through AMP?activated protein kinase activation in LPS?stimulated THP?1 cells
Role of ESAT-6 in renal injury by regulating microRNA-155 expression via TLR4/MyD88 signaling pathway in mice with Mycobacterium tuberculosis infection
Anticancer effect of curcumin inhibits cell growth through miR-21/PTEN/Akt pathway in breast cancer cell
B-cell specific Moloney leukemia virus insert site 1 and peptidyl arginine deiminase IV positively regulate carcinogenesis and progression of esophageal squamous cell carcinoma
Effects of microRNA-223 on morphine analgesic tolerance by targeting NLRP3 in a rat model of neuropathic pain
HSVtk/GCV system on hepatoma carcinoma cells: Construction of the plasmid pcDNA3.1-pAFP-TK and targeted killing effect
Role of SDF-1?/CXCR4 signaling pathway in clinicopathological features and prognosis of patients with nasopharyngeal carcinoma
Retinoid acid-induced microRNA-31-5p suppresses myogenic proliferation and differentiation by targeting CamkII?
Role of microRNA-130a in the pathogeneses of obstructive sleep apnea hypopnea syndrome-associated pulmonary hypertension by targeting the GAX gene
Green Tea Polyphenols, Mimicking the Effects of Dietary Restriction, Ameliorate High-Fat Diet-Induced Kidney Injury via Regulating Autophagy Flux
Expression and Clinicopathological Significance of Mel-18 and Bmi-1 in Esophageal Squamous Cell Carcinoma
Guangxi cobra venom-derived NGF promotes the osteogenic and therapeutic effects of porous BCP ceramic
Antioxidant axis Nrf2-keap1-ARE in inhibition of alcoholic liver fibrosis by IL-22
miR-21 enhances cardiac fibrotic remodeling and fibroblast proliferation via CADM1/STAT3 pathway
EZH2?mediated repression of Dkk1 promotes hepatic stellate cell activation and hepatic fibrosis
Resveratrol mitigates lipopolysaccharide-mediated acute inflammation in rats by inhibiting the TLR4/NF-?Bp65/MAPKs signaling cascade
Metabolomic mechanisms of gypenoside against liver fibrosis in rats: An integrative analysis of proteomics and metabolomics data
Wnt-?-catenin signaling pathway inhibition by sclerostin may protect against degradation in healthy but not osteoarthritic cartilage
In vivo and in vitro effects of microRNA-27a on proliferation, migration and invasion of breast cancer cells through targeting of SFRP1 gene via Wnt/?-catenin signaling pathway
Effects of 1,25(OH)2D3 and vitamin D receptor on peripheral CD4+/CD8+ double?positive T lymphocytes in a mouse model of systemic lupus erythematosus
?2-adrenoreceptor Inverse Agonist Down-regulates Muscarine Cholinergic Subtype-3 Receptor and Its Downstream Signal Pathways in Airway Smooth Muscle Cells in vitro
Acute effect of lactic acid on tumor-endothelial cell metabolic coupling in the tumor microenvironment
Effects of piezosurgery in accelerating the movement of orthodontic alveolar bone tooth of rats and the expression mechanism of BMP-2
Effect of Phosphatase and Tensin Homologue on Chromosome 10 on Angiotensin II-Mediated Proliferation, Collagen Synthesis, and Akt/P27 Signaling in Neonatal Rat Cardiac Fibroblasts
An APOC3 3'UTR variant associated with plasma triglycerides levels and coronary heart disease by creating a functional miR-4271 binding site
Upregulation of retinoic acid receptor-? reverses drug resistance in cholangiocarcinoma cells by enhancing susceptibility to apoptosis
The study on mechanism of the modified Chinese herbal compound, jianpijiedu, on a mouse model of hepatic carcinoma cachexia
Endothelial lipase is upregulated by interleukin-6 partly via the p38 MAPK and p65 NF-?B signaling pathways
Amarogentin Induces?Apoptosis?of Liver Cancer?Cells?via Upregulation of p53 and Downregulation of Human Telomerase Reverse Transcriptase in Mice
Epigenetics changes caused by the fusion of human embryonic stem cell and ovarian cancer cells
miR-138 suppresses cell proliferation and invasion by inhibiting SOX9 in hepatocellular carcinoma
Stromal cell-derived factor-1 promotes human adipose tissue-derived stem cell survival and chronic wound healing
p53 Mediates Colistin-Induced Autophagy and Apoptosis in PC-12 Cells
[Gly14]-Humanin Protects Against Amyloid ? Peptide-Induced Impairment of Spatial Learning and Memory in Rats
Protective effect of Astragaloside IV against sepsis-induced acute lung injury in rats
MicroRNAs-491-5p suppresses cell proliferation and invasion by inhibiting IGF2BP1 in non-small cell lung cancer
Polymorphisms in the CHIT1 gene: Associations with colorectal cancer
Protection Efficacy of the Extract of Ginkgo biloba against the Learning and Memory Damage of Rats under Repeated High Sustained +Gz Exposure
Anticancer Effects of 1,3-Dihydroxy-2-Methylanthraquinone and the Ethyl Acetate Fraction of Hedyotis Diffusa Willd against HepG2 Carcinoma Cells Mediated via Apoptosis
Hydrosulfide attenuates acute myocardial ischemic injury through the glycogen synthase kinase-3?/?-catenin signaling pathway
Expression and Distribution of the Guanine Nucleotide-binding Protein Subunit Alpha-s in Mice Skin Tissues and Its Association with White and Black Coat Colors
SPAG9 controls the cell motility, invasion and angiogenesis of human osteosarcoma cells
The survivin suppressant YM155 reverses doxorubicin resistance in osteosarcoma
Effects of diclofenac sodium and octreotide on treatment of caerulein-induced acute pancreatitis in mice
Resveratrol Protects PC12 Cell against 6-OHDA Damage via CXCR4 Signaling Pathway
Co-Inhibition of GLUT-1 Expression and the PI3K/Akt Signaling Pathway to Enhance the Radiosensitivity of Laryngeal Carcinoma Xenografts?In Vivo
The study of energy metabolism in bladder cancer cells in co-culture conditions using a microfluidic chip.
Regulation of the osteogenic and adipogenic differentiation of bone marrow-derived stromal cells by extracellular uridine triphosphate: The role of P2Y2 receptor and ERK1/2 signaling
Micheliolide overcomes KLF4-mediated cisplatin resistance in breast cancer cells by downregulating glutathione
Differential Expression of miR-34c and Its Predicted Target Genes in Testicular Tissue at Different Development Stages of Swine
GL-V9, a new synthetic flavonoid derivative, ameliorates DSS-induced colitis against oxidative stress by up-regulating Trx-1 expression via activation of AMPK/FOXO3a pathway
An investigation into the expression and mechanism of action of urotensin II in chronic pressure-overloaded rat hearts
Lefty-1 alleviates TGF-?1-induced fibroblast?myofibroblast transdifferentiation in NRK-49F cells
Apigenin inhibits the proliferation of adenoid cystic carcinoma via suppression of glucose transporter-1
Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer?s Disease-Like Amyloid-? Pathology and Neuroinflammation in the Rat Hippocampus
RLN2 Is a Positive Regulator of AKT-2-Induced Gene Expression Required for Osteosarcoma Cells Invasion and Chemoresistance
Octreotide Protects the Mouse Retina against Ischemic Reperfusion Injury through Regulation of Antioxidation and Activation of NF-?B
RUNX2 RNA interference inhibits the invasion of osteosarcoma
Cyclin-dependent kinase 11p110 (CDK11p110) is crucial for human breast cancer cell proliferation and growth
Mechanical Strain Regulates Osteogenic and Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells
Effects of corticosterone on the metabolic activity of cultured chicken chondrocytes
Connective tissue growth factor stimulates the proliferation, migration and differentiation of lung fibroblasts during paraquat-induced pulmonary fibrosis
Differential Expression of miR-499 and Validation of Predicted Target Genes in the Testicular Tissue of Swine at Different Developmental Stages
Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera
SATB1 Overexpression Regulates the Development and Progression in Bladder Cancer through EMT
A long-acting ?2-adrenergic agonist increases the expression of muscarine cholinergic subtype-3 receptors by activating the ?2-adrenoceptor cyclic adenosine monophosphate signaling pathway in airway smooth muscle cells
Autophagy Regulates Colistin-Induced Apoptosis in PC-12 Cells
Hypoxia-Inducible Factor-1? Protects Cervical Carcinoma Cells from Apoptosis Induced by Radiation via Modulation of Vascular Endothelial Growth Factor and p53 under Hypoxia
Resveratrol inhibits ?-amyloid-induced neuronal apoptosis via regulation of p53 acetylation in PC12 cells
Lycopene Attenuates Colistin-Induced Nephrotoxicity in Mice via Activation of the Nrf2/HO-1 Pathway
Lactobacillus plantarum?NDC 75017 alleviates the learning and memory ability in aging rats by reducing mitochondrial dysfunction
Overexpression of Wip1 Is Associated with Biologic Behavior in Human Clear Cell Renal Cell Carcinoma
Suppressor of Zeste 12 homolog RNA interference inhibits retinoblastoma cell invasion
Icariin promotes directed chondrogenic differentiation of bone marrow mesenchymal stem cells but not hypertrophy?in vitro
Acupuncture and moxibustion reduces neuronal edema in Alzheimer's disease rats
Baicalin inhibits colistin sulfate-induced apoptosis of PC12 cells
Knockdown of Aurora-B inhibits osteosarcoma cell invasion and migration via modulating PI3K/Akt/NF-?B signaling pathway
Apigenin suppresses GLUT-1 and p-AKT expression to enhance the chemosensitivity to cisplatin of laryngeal carcinoma Hep-2 cells: an?in vitro?study
TRPV4 Channel Inhibits TGF-?1-Induced Proliferation of Hepatic Stellate Cells
RIP3 overexpression sensitizes human breast cancer cells to parthenolide?in vitro?via intracellular ROS accumulation
Protective Effects of Lipoxin A4 in Testis Injury following Testicular Torsion and Detorsion in Rats
Overexpression of SATB1 Is Associated with Biologic Behavior in Human Renal Cell Carcinoma
Melittin Restores PTEN Expression by Down-Regulating HDAC2 in Human Hepatocelluar Carcinoma HepG2 Cells
MAGEC2, an epithelial-mesenchymal transition inducer, is associated with breast cancer metastasis
Caffeine Inhibits the Activation of Hepatic Stellate Cells Induced by Acetaldehyde via Adenosine A2A Receptor Mediated by the cAMP/PKA/SRC/ERK1/2/P38 MAPK Signal Pathway
Deciphering Molecular Mechanism Underlying Hypolipidemic Activity of Echinocystic Acid
The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer
Elevated Interleukin-32 Expression Is Associated with?Helicobacter pylori-Related Gastritis
Function of the transforming growth factor-?1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis
Inhibition of Aurora-B suppresses osteosarcoma cell migration and invasion
Celecoxib Ameliorates Non-Alcoholic Steatohepatitis in Type 2 Diabetic Rats via Suppression of the Non-Canonical Wnt Signaling Pathway Expression
Forsythiaside attenuates lipopolysaccharide-induced inflammatory responses in the bursa of Fabricius of chickens by downregulating the NF-?B signaling pathway
Effect of Antisense Oligodeoxynucleotides Glucose Transporter-1 on Enhancement of Radiosensitivity of Laryngeal Carcinoma
Resveratrol Inhibits ?-Amyloid-Induced Neuronal Apoptosis through Regulation of SIRT1-ROCK1 Signaling Pathway
Neuroprotective activities of catalpol against CaMKII-dependent apoptosis induced by LPS in PC12 cells
PLK1 gene suppresses cell invasion of undifferentiated thyroid carcinoma through the inhibition of CD44v6, MMP-2 and MMP-9
Let-7b Inhibits Human Cancer Phenotype by Targeting Cytochrome P450 Epoxygenase 2J2
Effects of acupuncture on Th1, th2 cytokines in rats of implantation failure
Role of bone morphogenetic proteins in form-deprivation myopia sclera
Over-expression of aldehyde dehydrogenase-2 protects against H2O2-induced oxidative damage and apoptosis in peripheral blood mononuclear cells
A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).
Effect of Buddleia flavonoids drug-containing plasma on the expression of STAT1 phosphoprotein in lacrimal gland epithelial cells in vitro
Role of bone morphogenetic proteins in form-deprivation myopia sclera
Over-expression of aldehyde dehydrogenase-2 protects against H2O2-induced oxidative damage and apoptosis in peripheral blood mononuclear cells
A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).
Effect of Buddleia flavonoids drug-containing plasma on the expression of STAT1 phosphoprotein in lacrimal gland epithelial cells in vitro
Jia L, Li F, Shao M, Zhang W, Zhang C, Zhao X, Luan H, Qi Y, Zhang P, Liang L, Jia X, Zhang K, Lu Y, Yang Z, Zhu X, Zhang Q, Du J, Wang W Oncol Lett. 2018
Wei X, Yu L, Kong X. Onco Targets Ther. 2018 Apr 18;11:2205-2216. doi: 10.2147/OTT.S156361. eCollection 2018. miR-488 inhibits cell growth and metastasis in renal cell carcinoma by targeting HMGN5
He YM, Zhang ZL, Liu QY, Xiao YS, Wei L, Xi C, Nan X. J Cell Mol Med. 2018 May;22(5):2569-2579. doi: 10.1111/jcmm.13499. Epub 2018 Mar 8. Effect of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells
Li Q, Ding L, Jing N, Liu C, Yang Z, Chen F, Hou L, Wang J. Oncol Lett. 2018 Feb;15(2):1963-1972. doi: 10.3892/ol.2017.7471. Epub 2017 Nov 22. Folate deficiency and aberrant DNA methylation and expression of FHIT gene were associated with cervical pathogenesis
An J, Zhang Z, Liu Z, Wang R, Hui D, Jin Y. BMC Cancer. 2017 Dec 6;17(1):828. doi: 10.1186/s12885-017-3839-7. Overexpression of Cullin7 is associated with hepatocellular carcinoma progression and pathogenesis
Du Y, Li J, Xu T, Zhou DD, Zhang L, Wang X. Oncotarget. 2017 Jun 22;8(37):61510-61527. doi: 10.18632/oncotarget.18604. eCollection 2017 Sep 22. MicroRNA-145 induces apoptosis of glioma cells by targeting BNIP3 and Notch signaling
Li B, Shi C, Zhao J, Li B. Oncotarget. 2017 Oct 7;8(54):92855-92863. doi: 10.18632/oncotarget.21612. eCollection 2017 Nov 3. Long noncoding RNA CCAT1 functions as a ceRNA to antagonize the effect of miR-410 on the down-regulation of ITPKB in human HCT-116 and HCT-8 cells
Xu C, Li X, Guo P, Wang J. Med Sci Monit. 2017 Oct 14;23:4909-4917. Hypoxia-Induced Activation of JAK/STAT3 Signaling Pathway Promotes Trophoblast Cell Viability and Angiogenesis in Preeclampsia
Xie XJ, Ma LG, Xi K, Fan DM, Li JG, Zhang Q, Zhang W. Mol Pain. 2017 Jan-Dec;13:1744806917706582. doi: 10.1177/1744806917706582. Effects of microRNA-223 on morphine analgesic tolerance by targeting NLRP3 in a rat model of neuropathic pain
Liu GF, Zhang SH, Li XF, Cao LY, Fu ZZ, Yu SN. Oncotarget. 2017 Aug 18;8(46):80757-80769. doi: 10.18632/oncotarget.20358. eCollection 2017 Oct 6. Overexpression of microRNA-132 enhances the radiosensitivity of cervical cancer cells by down-regulating Bmi-1
Zhang L, Xu C, Hu W, Wu P, Qin C, Zhang J. Int J Mol Med. 2018 Mar;41(3):1293-1304. doi: 10.3892/ijmm.2017.3327. Epub 2017 Dec 18. Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway
An Z, Wang D, Yang G, Zhang WQ, Ren J, Fu JL. Medicine (Baltimore). 2017 May;96(20):e6746. doi: 10.1097/MD.0000000000006746. Role of microRNA-130a in the pathogeneses of obstructive sleep apnea hypopnea syndrome-associated pulmonary hypertension by targeting the GAX gene
Liu LJ, Yu JJ, Xu XL. Am J Transl Res. 2017 Jul 15;9(7):3499-3513. eCollection 2017. MicroRNA-93 inhibits apoptosis and promotes proliferation, invasion and migration of renal cell carcinoma ACHN cells via the TGF-β/Smad signaling pathway by targeting RUNX3
Zhou C, Huang J, Li Q, Nie J, Xu X, Wang DW. Sci Rep. 2017 Jul 31;7(1):6926. doi: 10.1038/s41598-017-07512-1. Soluble Epoxide Hydrolase Inhibition Protected against Angiotensin II-induced Adventitial Remodeling
Bao W, Luo Y, Wang D, Li J, Wu X, Mei W. J Cell Biochem. 2018 Jan;119(1):850-860. doi: 10.1002/jcb.26249. Epub 2017 Aug 30. Sodium salicylate modulates inflammatory responses through AMP
Fang X, Han Q, Li S, Zhao Y, Luo A. Am J Transl Res. 2017 Sep 15;9(9):4288-4299. eCollection 2017. Chikusetsu saponin IVa attenuates isoflurane-induced neurotoxicity and cognitive deficits via SIRT1/ERK1/2 in developmental rats
Dai B, Zhu F, Chen Y, Zhou R, Wang Z, Xie Y, Wu X, Zu S, Li G, Ge J0, Chen F. Int J Mol Sci. 2017 Oct 11;18(10). pii: E2125. doi: 10.3390/ijms18102125. ASIC1a Promotes Acid-Induced Autophagy in Rat Articular Chondrocytes through the AMPK/FoxO3a Pathway
Liu S, Li X, Wu Y, Duan R, Zhang J, Du F, Zhang Q, Li Y, Li N. PLoS One. 2017 Dec 14;12(12):e0189722. doi: 10.1371/journal.pone.0189722. eCollection 2017. Effects of vaspin on pancreatic β cell secretion via PI3K/Akt and NF-κB signaling pathways
Kong LY, Xue M, Zhang QC, Su CF. Oncotarget. 2017 Feb 28;8(9):15507-15519. doi: 10.18632/oncotarget.14662. In vivo and in vitro effects of microRNA-27a on proliferation, migration and invasion of breast cancer cells through targeting of SFRP1 gene via Wnt/β-catenin signaling pathway
Liu B, Liu C, Cong W, Li N, Zhou N, Tang Y, Wei C, Bai H, Zhang Y, Xiao J. Skelet Muscle. 2017 May 11;7(1):8. doi: 10.1186/s13395-017-0126-x. Retinoid acid-induced microRNA-31-5p suppresses myogenic proliferation and differentiation by targeting CamkIIδ
Wang R, Shen Z, Yang L, Yin M, Zheng W, Wu B, Liu T, Song H. Int J Mol Med. 2017 Nov;40(5):1537-1548. doi: 10.3892/ijmm.2017.3121. Epub 2017 Sep 6. Protective effects of heme oxygenase-1-transduced bone marrow-derived mesenchymal stem cells on reduced-size liver transplantation: Role of autophagy regulated by the ERK/mTOR signaling pathway
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Customer Q&As

Q: What is the correct storage temperature of this kit? On the manual it says room temperature, but in the manual that we received in the past it said 4 degrees.
A: We have modified the storage temperature of this kit as room temperature recently, but it can be stored at 4 degree as well. The BSA solution contains sodium azide, it is available at room temperature.