Show Order Info
SKU AR1023
Pack size 1 kit (20X)
Applications Immunohistochemistry

List of Components

DescriptionQuantityVolumeCatalog Number
Chromogenic Reagent A: BCIP/NBT Concentrated Solution13mL (Dilution: 1:20)AR1023-A
Chromogenic Reagent B: TRIS Concentrated Buffer (pH9.4)13mL (Dilution: 1:20)AR1023-B

Overview

Product Name BCIP/ NBT Chromogenic Substrate Kit
SKU/Catalog Number AR1023
Pack Size 1 kit (20X)
Form Supplied Concentrated solution (20X)
Storage Upon receipt store BCIP/ NBT Chromogenic Substrate Kit at -20°C. It is stable at -20°C for one year.
Equivalent Abcam (Product No. ab7468, ab7413)
Application Immunohistochemistry
*Our Boster Guarantee covers the use of this product in the above tested applications.
Description Boster’s BCIP/ NBT Chromogenic Substrate Kit is ready-made solutions for chromogenic blot and immunohistochemical staining applications.ant applied directly to fluorescently labeled cells or tissue samples on microscope slides.
Cite This Product BCIP/ NBT Chromogenic Substrate Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1023)

Assay Principle

Boster’s BCIP/ NBT Chromogenic Substrate Kit are ready-made solutions for chromogenic blot and immunohistochemical staining applications. NBT nitro-blue tetrazolium and BCIP 5-bromo-4-chloro-3'-indolyphosphate results in an intense, black purple precipitate after reacting with alkaline phosphatase.

Assay Protocol

1. After the alkaline phosphatase detection, wash fully with TBS 4 times and each time for 5 minutes. Do not use phosphate buffer.

2. Add one drop of chromogenic reagent A and B respectively into 1ml of distilled water and mix evenly. Add it to the sample and colorate in dark for 10-30 minutes. If no background appearing, keep coloration until satisfied.

3. Wash fully with distilled water to stop reaction. Counterstain with nuclear fast red if necessary.

4. Seal sllide with Aqueous Mounting Medium (AR1018). Observe with microscope

BCIP/ NBT Chromogenic Substrate Kit Images

Click the images to enlarge.

BCIP/ NBT Chromogenic Substrate Kit
Write a review for AR1023

Publications

MicroRNA-608 inhibits proliferation of bladder cancer via AKT/FOXO3a signaling pathway
Glycogen synthase kinase?3? is required for epithelial?mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions
Amarogentin Induces?Apoptosis?of Liver Cancer?Cells?via Upregulation of p53 and Downregulation of Human Telomerase Reverse Transcriptase in Mice
Protective effect of histamine microinjected into the cerebellar fastigial nucleus on stress-induced gastric mucosal damage in rats
Cartilage Oligomeric Matrix Protein Gene Multilayers Inhibit Osteogenic Differentiation and Promote Chondrogenic Differentiation of Mesenchymal Stem Cells
Proteomic Analysis of?Apis cerana?and?Apis mellifera?Larvae Fed with Heterospecific Royal Jelly and by CSBV Challenge
DRP-1, ezrin and E-cadherin expression and the association with esophageal squamous cell carcinoma
Glutamate microinjection into the hypothalamic paraventricular nucleus attenuates ulcerative colitis in rats
A convenient system for highly specific and sensitive detection of miRNA expression
Effect of Aspirin on the Expression of Hepatocyte NF-?B and Serum TNF-? in Streptozotocin-Induced Type 2 Diabetic Rats
Significance and relationship between DJ-1 gene and survivin gene expression in laryngeal squamous cell carcinoma
Effect of Aspirin on the Expression of Hepatocyte NF-?B and Serum TNF-? in Streptozotocin-Induced Type 2 Diabetic Rats
Significance and relationship between DJ-1 gene and survivin gene expression in laryngeal squamous cell carcinoma
Zhao JR, Cheng WW, Wang YX, Cai M, Wu WB, Zhang HJ. Cell Death Dis. 2018 Jan 24;9(2):94. doi: 10.1038/s41419-017-0108-2. Identification of microRNA signature in the progression of gestational trophoblastic disease.
Liang, Z., Wang, X., Xu, X., Xie, B., Ji, A., Meng, S.,…, & Liu, B. (2017). MicroRNA-608 inhibits proliferation of bladder cancer via AKT/FOXO3a signaling pathway. Molecular Cancer, 16(1), 96. doi: 10.1186/s12943-017-0664-1