BCIP/ NBT Chromogenic Substrate Kit

antibody

Boster’s BCIP/ NBT Chromogenic Substrate Kit is ready-made solutions for chromogenic blot and immunohistochemical staining applications. Cited in 31 publication(s).

Product Info Summary

SKU: AR1023
Size: 1kit

List of Components

DescriptionQuantityVolumeCatalog Number
Chromogenic Reagent A: BCIP/NBT Concentrated Solution13mL (Dilution: 1:20)AR1023-A
Chromogenic Reagent B: TRIS Concentrated Buffer (pH9.4)13mL (Dilution: 1:20)AR1023-B

Overview

Product Name BCIP/ NBT Chromogenic Substrate Kit
SKU/Catalog Number AR1023
Pack Size 1 kit (20X)
Form Supplied Concentrated solution (20X)
Storage Upon receipt store BCIP/ NBT Chromogenic Substrate Kit at -20°C. It is stable at -20°C for one year.
Equivalent Abcam (Product No. ab7468, ab7413)
Application Immunohistochemistry
*Our Boster Guarantee covers the use of this product in the above tested applications.
Description Boster’s BCIP/ NBT Chromogenic Substrate Kit is ready-made solutions for chromogenic blot and immunohistochemical staining applications.ant applied directly to fluorescently labeled cells or tissue samples on microscope slides.
Cite This Product BCIP/ NBT Chromogenic Substrate Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1023)

Assay Principle

Boster’s BCIP/ NBT Chromogenic Substrate Kit are ready-made solutions for chromogenic blot and immunohistochemical staining applications. NBT nitro-blue tetrazolium and BCIP 5-bromo-4-chloro-3'-indolyphosphate results in an intense, black purple precipitate after reacting with alkaline phosphatase.

Assay Protocol

1. After the alkaline phosphatase detection, wash fully with TBS 4 times and each time for 5 minutes. Do not use phosphate buffer.

2. Add one drop of chromogenic reagent A and B respectively into 1ml of distilled water and mix evenly. Add it to the sample and colorate in dark for 10-30 minutes. If no background appearing, keep coloration until satisfied.

3. Wash fully with distilled water to stop reaction. Counterstain with nuclear fast red if necessary.

4. Seal sllide with Aqueous Mounting Medium (AR1018). Observe with microscope

Validation Images & Assay Conditions

AR1023 has been cited in 31 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Protective effect of histamine microinjected into the cerebellar fastigial nucleus on stress-induced gastric mucosal damage in rats

Suppression of CDK2 expression by siRNA induces cell cycle arrest and cell proliferation inhibition in human cancer cells

GSK-3β inhibitor attenuates urinary albumin excretion in type 2 diabetic db/db mice, and delays epithelial-to-mesenchymal transition in mouse kidneys and podocytes

A lncRNA-miRNA-mRNA network for human primed, naive and extended pluripotent stem cells

Amarogentin Induces Apoptosis of Liver Cancer Cells via Upregulation of p53 and Downregulation of Human Telomerase Reverse Transcriptase in Mice:

Effects of Cartilage Oligomeric Matrix Protein on Bone Morphogenetic Protein‐2‐induced Differentiation of Mesenchymal Stem Cells

MicroRNA-493 targets STMN-1 and promotes hypoxia-induced epithelial cell cycle arrest in G2/M and renal fibrosis

Muscimol microinjection into cerebellar fastigial nucleus exacerbates stress-induced gastric mucosal damage in rats

Detecting miRNAs by liquid hybridization and color development

Cyclooxygenase-2 and vascular endothelial growth factor expressions are involved in ultrafiltration failure

The homologous genes Vangl1 and Vangl2 are required for embryo implantation in the uterus of mice during early pregnancy

Development of a novel RNAi therapy: Engineered miR-31 exosomes promoted the healing of diabetic wounds

MicroRNA-361-5p facilitates cervical cancer progression through mediation of epithelial-to-mesenchymal transition

Construction of Targeting Vector for Expressing Human GDNF in Cattle Mammary Gland

Isolation and characterization of mesenchymal stem cells from chicken bone marrow

MicroRNA-608 inhibits proliferation of bladder cancer via AKT/FOXO3a signaling pathway

Hu M,Wu Y,Yang C,Wang X,Wang W,Zhou L,Zeng T,Zhou J,Wang C,Lao G,Yan L,Ren M.Novel Long Noncoding RNA lnc-URIDS Delays Diabetic Wound Healing by Targeting Plod1.Diabetes.2020 Oct;69(10):2144-2156.doi:10.2337/db20-0147.Epub 2020 Aug 14.PMID:32801140.
Species: Rat
AR1023 usage in article: APP:ISH, SAMPLE:SKIN TISSUE, DILUTION:NA

Wang H,Wang L,Zhou X,Luo X,Liu K,Jiang E,Chen Y,Shao Z,Shang Z.OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway.Biomed Res Int.2020 Jul 8;2020:2125656.doi:10.1155/2020/2125656.PMID:32695810;PMCID:PMC7368228.
Species: Human
AR1023 usage in article: APP:ISH, SAMPLE:MUCOSA TISSUE, DILUTION:NA

Jinghuan Huang,Muyu Yu,Wenjing Yin,Bo Liang,Ang Li,Jingfeng Li,Xiaolin Li,Shichang Zhao,Fang Liu,Development of a novel RNAi therapy: Engineered miR-31 exosomes promoted the healing of diabetic wounds,Bioactive Materials,Volume 6,Issue 9,2021,Pages 2841-2
Species: Human,Rat
AR1023 usage in article: APP:IN SITU HYBRIDIZATION, SAMPLE:WOUND TISSUE, DILUTION:NA

Glycogen synthase kinase?3? is required for epithelial?mesenchymal transition and barrier dysfunction in mouse podocytes under high glucose conditions

Amarogentin Induces?Apoptosis?of Liver Cancer?Cells?via Upregulation of p53 and Downregulation of Human Telomerase Reverse Transcriptase in Mice

Protective effect of histamine microinjected into the cerebellar fastigial nucleus on stress-induced gastric mucosal damage in rats

DRP-1, ezrin and E-cadherin expression and the association with esophageal squamous cell carcinoma

Effect of Aspirin on the Expression of Hepatocyte NF-?B and Serum TNF-? in Streptozotocin-Induced Type 2 Diabetic Rats

Zhao JR, Cheng WW, Wang YX, Cai M, Wu WB, Zhang HJ. Cell Death Dis. 2018 Jan 24;9(2):94. doi: 10.1038/s41419-017-0108-2. Identification of microRNA signature in the progression of gestational trophoblastic disease.

Liang, Z., Wang, X., Xu, X., Xie, B., Ji, A., Meng, S.,…, & Liu, B. (2017). MicroRNA-608 inhibits proliferation of bladder cancer via AKT/FOXO3a signaling pathway. Molecular Cancer, 16(1), 96. doi: 10.1186/s12943-017-0664-1

Li Tt, Zhang Jf, Fei Sj, Zhu Sp, Zhu Jz, Qiao X, Liu Zb. Acta Pharmacol Sin. 2014 Feb;35(2):185-94. Doi: 10.1038/Aps.2013.140. Epub 2013 Dec 23. Glutamate Microinjection Into The Hypothalamic Paraventricular Nucleus Attenuates Ulcerative Colitis I...

Shen Z, Ren Y, Ye D, Guo J, Kang C, Ding H. Eur J Histochem. 2011 Mar 21;55(1):E9. Doi: 10.4081/Ejh.2011.E9. Significance And Relationship Between Dj-1 Gene And Surviving Gene Expression In Laryngeal Carcinoma.

Guo P, Shi Zl, Liu A, Lin T, Bi Fg, Shi Mm, Yan Sg. Int J Mol Sci. 2014 Nov 5;15(11):20117-33. Doi: 10.3390/Ijms151120117. Cartilage Oligomeric Matrix Protein Gene Multilayers Inhibit Osteogenic Differentiation And Promote Chondrogenic Differentia...

Li X, Ni M, Zhang C, Ma W, Zhang Y. Rna. 2014 Feb;20(2):252-9. Doi: 10.1261/Rna.040220.113. Epub 2013 Dec 17. A Convenient System For Highly Specific And Sensitive Detection Of Mirna Expression.

Zhang Y, Zhang G, Huang X, Han R. Plos One. 2014 Aug 7;9(8):E102663. Doi: 10.1371/Journal.Pone.0102663. Ecollection 2014. Proteomic Analysis Of Apis Cerana And Apis Mellifera Larvae Fed With Heterospecific Royal Jelly And By Csbv Challenge.

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