Beta-human Chorionic Gonadotropin(β-hCG) ELISA Kit

ELISA kit

Sandwich High Sensitivity ELISA kit for Quantitative Detection of β-hCG. 96 wells/kit.

Product Info Summary

SKU: EK7062
Size: 96 wells/kit
Reactive Species: Human
Application: ELISA
Sample Types: Serum and Urine

Product Name

Beta-human Chorionic Gonadotropin(β-hCG) ELISA Kit

SKU/Catalog Number

EK7062

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of β-hCG. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Beta-human Chorionic Gonadotropin(β-hCG) ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7062)

Clonality of Antibodies

See Datasheet for details

Sensitivity

2.0 IU/L

Assay Range

8-240 IU/L

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7062 is reactive to in Human samples

Validated Sample Types

Serum and Urine

Application Guarantee

EK7062 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

DescriptionQuantityVolumeBuffers
Anti-hCG-β Pre-coated 96-well strip microplate18 strips of 12 wellsAnti-hCG-β monoclonal antibody, Polystyrene micro-well plate
hCG-β Standards (S0~S5)60.5mlhCG-β (0, 8, 16, 40, 100, 240 IU/L), 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300
HRP Conjugated anti-hCG-β antibody16mlHRP Conjugated anti-hCG-β antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1%Proclin-300
Controls20.5mlhCG-β, 100% natural protein, 0.1%Proclin-300
10 X Urine Sample Dilute Buffer Concentrate115ml0.2M PBS, 20% new-born calf serum, 0.1% Proclin-300
20X Wash Buffer Concentrate115ml0.2M PBS containing 0.5% tween 20
Color Developing Reagent A17ml11m mol/L Urea hydrogen peroxide
Color Developing Reagent B17ml2m mol/L 3,3'5,5’-Tetramethylbenzidine
Stop Solution17ml2mol/L Sulphuric acid
Plate Sealers2Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Data Example Images

Gene/Protein Information For (Source: Uniprot.org, NCBI)

Gene Name

Full Name

Weight

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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2 Customer Q&As for Beta-human Chorionic Gonadotropin(β-hCG) ELISA Kit

Question

One of our customer is interested in ELISA Kit # EK7062. They are wondering if standard for this kit is recombinant HCG protein or other protein source. Is it possible to know what are the standards exactly ?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

The source of the standard for EK7062 is natural protein extracted from serum and urine of pregnant woman.

Boster Scientific Support

Answered: 2020-01-10

Question

Does this hCG ELISA kit (EK7062) recognizes intact only or total hCG?

Verified Customer

Verified customer

Asked: 2014-10-06

Answer

EK7062 recognizes the whole biologically active molecule which is the intact hCG. 

Boster Scientific Support

Answered: 2014-10-06

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