Mouse Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin Conjugated

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all mouse serum proteins, except the specific antibody for rabbit IgG. Cited in 10 publication(s).

Product Info Summary

SKU: BM2004
Size: 0.5ml
Reactive Species: Rabbit
Host: Mouse
Application: ELISA, IHC

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Product Overview

Product Name Mouse Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin Conjugate
Synonyms Biotin-conjugated Mouse Anti-Rabbit IgG; Mouse Anti-Rabbit IgG Biotinylated Antibody; Biotinylated Mouse Anti-Rabbit IgG Secondary Antibody; Mouse Anti-Rabbit IgG Secondary Antibody, Biotin-labeled
Description Mouse Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin Conjugate, for indirect sensitive immunodetection and/or quantification of low-abundance target proteins through ELISA or IHC by using reporter-labeled biotin-binding signal amplification systems.
Reagent Type Biotin conjugated secondary antibody
Conjugate Biotin
Host Mouse
Target Species Rabbit
Antibody Class IgG
Clonality Polyclonal
Immunogen Whole molecule rabbit IgG
Purification Immunoaffinity chromatography
Specificity Rabbit IgG specific; no cross-reactivity with human/goat/bovine IgG
Form Supplied Liquid: concentrated buffered stock solution
Formulation 0.5 mg biotin-conjugated secondary antibody
0.01 M PBS (PH 7.4)
50% glycerol
Pack Size 0.5 ml
Concentration 1 mg/ml
Application ELISA, IHC
Storage At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type Human primary-antibody-probed: SDS-PAGE separated-, membrane-immobilized-proteins from cell/tissue lysates, formalin-fixed paraffin-embedded (FFPE) tissue sections on slides
Assay Type Immunoanalytical
Technique Indirect immunodetection of target protein via reporter-labeled biotin-binding detection systems
Assay Purpose Protein detection/quantification
Equipment Needed WB/Dot blot/ELISA/IHC instrumentation; Reporter signal detectors: X-ray film cassette; a charge-coupled device (CCD) imager; Spectrophotometer; fluorescent or electron microscope

Main Advantages

Specific High signal-to-noise ratio
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody; Multiple reporter molecules localize to a single biotin via avidin/streptavidin bridges
Fast Fewer processing steps - no need to add a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; Fluorescence can be observed directly
Quantifieable Allows quantification of detected signal
Easy to Use Supplied in a workable liquid format
Flexible Biotin- (Strept)Avidin system can be coupled with various types of reporters (enzymes, fluorochromes, fluorophores, chromophores, etc.); One type of labeled secondary antibody can be used to recognize different types of primary antibodies of the target organism specific to a particular antigen
Compatible Biotin does not interfere with catalysis or antibody binding

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Biotinylated antibodies are widely used in systems where signal amplification is desired. Often 15-20 biotin moieties are coupled to a single IgG secondary antibody. Biotin binds avidin, streptavidin, or neutravidin with a high degree of affinity and specificity. In immunoassays avidin/streptavidin-biotin binding is used as a bridge between antibodies and reporters like enzymes (HRP, AP), fluorophores, chromophores, etc. Both avidin and streptavidin are tetrameric proteins capable of binding 4 biotin groups to each molecule of avidin or streptavidin, thus amplifying the signal intensity and detection sensitivity by increasing the concentration of reporters at the antigenic site. Two main biotin-binding detection systems have been widely utilized: Avidin-Biotin Complex (ABC) and Labeled Streptavidin Biotin (LSAB) methods. In the ABC method free avidin (or streptavidin) is used as a bridge/link between the biotinylated antibody and а biotinylated reporter molecule, resulting in three reporter molecules coupled to the biotinylated antibody. The LSAB method employs a reporter-labeled streptavidin (avidin or neutravidin can alternatively be used) to detect the bound biotinylated-secondary antibody on the tissue section, blotting membrane or ELISA plate, improving the sensitivity of detection by 8-fold. The LSAB method is used when the avidin-biotin-enzyme complex in the ABC method becomes too large to penetrate the tissue.

Validation Images & Assay Conditions

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BM2004 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Autologous stem cell transplantation in EBV-positive post-renal transplant refractory multiple myeloma: A case report and literature review

The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model

IL‑21/IL‑21R inhibit tumor growth and invasion in non‑small cell lung cancer cells via suppressing Wnt/β‑catenin signaling and PD‑L1 expression

Resveratrol inhibits the IL-1β-induced expression of MMP-13 and IL-6 in human articular chondrocytes via TLR4/MyD88-dependent and -independent signaling cascades

Curcumin improves perfusion recovery in experimental peripheral arterial disease by upregulating microRNA‑93 expression

Identification of Telocytes in the Pancreas of Turtles—A role in Cellular Communication

Melatonin prevents senescence of canine adipose-derived mesenchymal stem cells through activating NRF2 and inhibiting ER stress

Abdominal paracentesis drainage protects rats against severe acute pancreatitis-associated lung injury by reducing the mobilization of intestinal XDH/XOD

Intrafollicular expression and potential regulatory role of cocaine- and amphetamine-regulated transcript in the ovine ovary

Effects of varying tissue sizes on the efficiency of baboon ovarian tissue vitrification

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