|Product name||Broad Spectrum Phosphatase Inhibitor Cocktail|
|Pack size||1 mL|
|Applications||WB sample preparation, protein purification, Co-IP, protein kinase activity assay experiments|
|Form Supplied||100X concentrated stock solution|
|Physical State||Liquid, colorless to light yellow|
|Pack Size||1 mL|
|Content||Proprietary mix of: Sodium fluoride, Sodium orthavanadium, Imidazole, Sodium molybdate, Sodium sulphate, Sodium pyrophosphate, B-phosphoric acid glycerol|
|Recommended working concentration|| 100-fold dilution in tissue lysis buffer; |
Use 10 µl of the Phosphatase Inhibitor Cocktail solution to inhibit dephosphorylation of proteins for 1 ml of lysate
|Storage|| Upon receipt store at -20°C. |
It is stable at -20°C for one year. It is stable at 4°C for two months. Product is shipped on ice.
|Equivalent||Thermofisher (Product No.78428, A32957); Millipore Sigma (Product No. P5726)|
|Target Specificity||Tyrosine phosphatase, acidic and alkaline phosphatase; Serine/threonine phosphatase, histidine phosphatase, etc.|
|Sodium fluoride||Ser/Thr and acid phosphatases|
|Sodium orthavanadium|| ATPases |
Tyr and alkaline phosphatases
|Sodium molybdate||Acid and phosphoprotein phosphatases|
|Sodium tartrate||Acid phosphatase|
|Sodium pyrophosphate||Ser/Thr phosphatases|
|B-phosphoric acid glycerol||Ser/Thr phosphatases|
Broad Spectrum Phosphatase Inhibitor Cocktail is a Western blot related concentrated stock solution reagent containing a mixture of different phosphatase inhibitors that is to be added to cell lysis buffer to protect native phosphoproteins from dephosphorylation during proteins purification and sample preparation used in WB, Co-IP, ChIP, and protein kinase assays.
|Description||Broad Spectrum Phosphatase Inhibitor Cocktail is a Western blot related concentrated stock solution reagent containing a mixture of six phosphatase inhibitors that is to be added to cell lysis buffer to protect native phosphoproteins from dephosphorylation during proteins purification and sample preparation used in WB, Co-IP, ChIP, and protein kinase assays.|
|Cite This Product||Broad Spectrum Phosphatase Inhibitor Cocktail (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1183)|
|Application||WB sample preparation, protein purification, Co-IP, protein kinase activity assay experiments
Our Boster Guarantee covers the use of this product in the above tested applications.
|Reagent Type||Western Blotting related reagent; Inhibitors|
|Usage||To preserve phosphorylation state and protein functionality following cell lysis|
|Target Sample||Cell lysis extracts|
|Compatibility with assays|| Compatible with IEF/2D studies; |
MS-compatible: not contain AEBSF;
Compatible with immobilized metal chelate affinity chromatography and 2D gel electrophoresis inhibitor components dialysis removal or desalting;
Usage and Handling
The product is supplied as a 100X concentrated stock solution in a liquid format for improved accuracy, solubility, and ease of use in comparison to traditional tablets. Since phosphatase levels may vary among cell and tissue types, it may be necessary to increase the concentration of inhibitors.
Phosphatase inhibitors are used when phosphorylation (activation) states of target proteins need to be studied and the phosphorylated residues of interest must remain intact. They are chemicals that aid in the extraction of intact proteins in their native modification state by inhibiting endogenous phosphatases that would otherwise dephosphorylate the proteins present in cell lysates and tissue extracts.
Broad Spectrum Phosphatase Inhibitor Cocktail contains individual components with specific inhibitory properties to provide an all-around protection of the protein phosphorylation state. The six phosphatase inhibitors included in this mixture target a broad spectrum phosphatase categories.
Dynamic protein phosphorylation is a key cellular signaling mechanism for cell processes regulation. When tissues are lysed to make whole cell extracts, the loss of natural compartmentalization causes normal regulation of cellular signaling to get distorted, and resident cell phosphatases within the cell extract are free to disorderly dephosphorylate proteins. The usual consequence of this unregulated state is biologically meaningless representation of protein activities (i.e. phosphorylation status) and false negative staining in anti-phosphoprotein immunostaining analyses. The addition of phosphatase inhibitors to the cell lysis buffer aids in the preservation of phosphorylated residues at the time of cell disruption.
Broad Spectrum Phosphatase Inhibitor Cocktail Images
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