Click image to see more details
Product Info Summary
|Product name||Broad Spectrum Phosphatase Inhibitor Cocktail|
|Pack size||1 mL|
|Applications||WB sample preparation, protein purification, Co-IP, protein kinase activity assay experiments|
|Form Supplied||100X concentrated stock solution|
|Physical State||Liquid, colorless to light yellow|
|Pack Size||1 mL|
|Content||Proprietary mix of: Sodium fluoride, Sodium orthavanadium, Imidazole, Sodium molybdate, Sodium sulphate, Sodium pyrophosphate, B-phosphoric acid glycerol|
|Recommended working concentration|| 100-fold dilution in tissue lysis buffer; |
Use 10 µl of the Phosphatase Inhibitor Cocktail solution to inhibit dephosphorylation of proteins for 1 ml of lysate
|Storage|| Upon receipt store at -20°C. |
It is stable at -20°C for one year. It is stable at 4°C for two months. Product is shipped on ice.
|Equivalent||Thermofisher (Product No.78428, A32957); Millipore Sigma (Product No. P5726)|
|Target Specificity||Tyrosine phosphatase, acidic and alkaline phosphatase; Serine/threonine phosphatase, histidine phosphatase, etc.|
|Sodium fluoride||Ser/Thr and acid phosphatases|
|Sodium orthavanadium|| ATPases |
Tyr and alkaline phosphatases
|Sodium molybdate||Acid and phosphoprotein phosphatases|
|Sodium tartrate||Acid phosphatase|
|Sodium pyrophosphate||Ser/Thr phosphatases|
|B-phosphoric acid glycerol||Ser/Thr phosphatases|
Broad Spectrum Phosphatase Inhibitor Cocktail is a Western blot related concentrated stock solution reagent containing a mixture of different phosphatase inhibitors that is to be added to cell lysis buffer to protect native phosphoproteins from dephosphorylation during proteins purification and sample preparation used in WB, Co-IP, ChIP, and protein kinase assays.
|Description||Broad Spectrum Phosphatase Inhibitor Cocktail is a Western blot related concentrated stock solution reagent containing a mixture of six phosphatase inhibitors that is to be added to cell lysis buffer to protect native phosphoproteins from dephosphorylation during proteins purification and sample preparation used in WB, Co-IP, ChIP, and protein kinase assays.|
|Cite This Product||Broad Spectrum Phosphatase Inhibitor Cocktail (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1183)|
|Application||WB sample preparation, protein purification, Co-IP, protein kinase activity assay experiments
Our Boster Guarantee covers the use of this product in the above tested applications.
|Reagent Type||Western Blotting related reagent; Inhibitors|
|Usage||To preserve phosphorylation state and protein functionality following cell lysis|
|Target Sample||Cell lysis extracts|
|Compatibility with assays|| Compatible with IEF/2D studies; |
MS-compatible: not contain AEBSF;
Compatible with immobilized metal chelate affinity chromatography and 2D gel electrophoresis inhibitor components dialysis removal or desalting;
Usage and Handling
The product is supplied as a 100X concentrated stock solution in a liquid format for improved accuracy, solubility, and ease of use in comparison to traditional tablets. Since phosphatase levels may vary among cell and tissue types, it may be necessary to increase the concentration of inhibitors.
Phosphatase inhibitors are used when phosphorylation (activation) states of target proteins need to be studied and the phosphorylated residues of interest must remain intact. They are chemicals that aid in the extraction of intact proteins in their native modification state by inhibiting endogenous phosphatases that would otherwise dephosphorylate the proteins present in cell lysates and tissue extracts.
Broad Spectrum Phosphatase Inhibitor Cocktail contains individual components with specific inhibitory properties to provide an all-around protection of the protein phosphorylation state. The six phosphatase inhibitors included in this mixture target a broad spectrum phosphatase categories.
Dynamic protein phosphorylation is a key cellular signaling mechanism for cell processes regulation. When tissues are lysed to make whole cell extracts, the loss of natural compartmentalization causes normal regulation of cellular signaling to get distorted, and resident cell phosphatases within the cell extract are free to disorderly dephosphorylate proteins. The usual consequence of this unregulated state is biologically meaningless representation of protein activities (i.e. phosphorylation status) and false negative staining in anti-phosphoprotein immunostaining analyses. The addition of phosphatase inhibitors to the cell lysis buffer aids in the preservation of phosphorylated residues at the time of cell disruption.
Click image to see more details
AR1183 has been cited in 26 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lin X,Bai D,Wei Z,Zhang Y,Huang Y,Deng H,Huang X.Curcumin attenuates oxidative stress in RAW264.7 cells by increasing the activity of antioxidant enzymes and activating the Nrf2-Keap1 pathway.PLoS One.2019 May 21;14(5):e0216711.doi:10.1371/journal.pone.0216711.PMID:31112588;PMCID:PMC6528975.
AR1183 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA
Ren Y,Jiang J,Jiang W,Zhou X,Lu W,Wang J,Luo Y.Spata2 Knockdown Exacerbates Brain Inflammation via NF-κB/P38MAPK Signaling and NLRP3 Inflammasome Activation in Cerebral Ischemia/Reperfusion Rats.Neurochem Res.2021 Jun 1.doi:10.1007/s11064-021-03360-8.Epub ahead of print.PMID:34075523.
AR1183 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA
Zhang T,Liu C,Yu Y,Geng J,Meng Q,Xu S,Zhou F,Chen Y,Jin S,Shen J,Pan B,Meng F,Liu F. TBL1XR1 is involved in c-Met-mediated tumorigenesis of human nonsmall cell lung cancer.Cancer Gene Ther.2020 Apr;27(3-4):136-146.doi:10.1038/s41417-019-0111-0.Epub 2019 Jun 27.PMID:31243347.
AR1183 usage in article: APP:WB, SAMPLE:NSCLC CELL, DILUTION:NA
Wang, X.,Wang, J.,Huang, G.,Li, Y.,& Guo, S. (2021). miR‑320a‑3P alleviates the epithelial‑mesenchymal transition of A549 cells by activation of STAT3/SMAD3 signaling in a pulmonary fibrosis model. Molecular Medicine Reports,23,357.https://doi.org/10.3892/mmr.2021.11996
AR1183 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:NA
Guo J,Ren R,Yao X,Ye Y,Sun K,Lin J,Wang G, Guo F,Xiao J,Xu T.PKM2 suppresses osteogenesis and facilitates adipogenesis by regulating β-catenin signaling and mitochondrial fusion and fission.Aging (Albany NY).2020 Feb 25;12(4):3976-3992.doi:10. 18632/aging
AR1183 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:NA
Chen R,Guo S,Yang C,Sun L,Zong B,Li K,Liu L,Tu G,Liu M,Liu S.Although c‑MYC contributes to tamoxifen resistance, it improves cisplatin sensitivity in ER‑positive breast cancer.Int J Oncol.2020 Apr;56(4):932-944.doi:10.3892/ijo.2020.4987.Epub 2020 Feb 14.P
Chu X,Yu T,Huang X,Xi Y,Ni B,Zhang R,You H.Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats.Aging (Albany NY).2020 Jul 5;12(13):12799-12811.doi:10.18632/aging.103222.Epub 2020
AR1183 usage in article: APP:WB, SAMPLE:PRIMARY CHONDROCYTES, DILUTION:NA
Zhang R,Ji J,Zhou X,Li R.Irisin Pretreatment Protects Kidneys against Acute Kidney Injury Induced by Ischemia/Reperfusion via Upregulating the Expression of Uncoupling Protein 2. Biomed Res Int.2020 Aug 31;2020:6537371.doi:10.1155/2020/6537371.PMID:329349
AR1183 usage in article: APP:WB, SAMPLE:HK-2 CELL AND KIDNEY TISSUE, DILUTION:NA
Wang C,Sun Z,Zhao C,Zhang Z,Wang H,Liu Y,Guo Y,Zhang B,Gu L,Yu Y,Hu Y,Wu J.Maintaining manganese in tumor to activate cGAS-STING pathway evokes a robust abscopal anti-tumor effect.J Control Release.2021 Feb 2:S0168-3659(21)00045-6.doi:10.1016/j. jconrel.2
AR1183 usage in article: APP:WB, SAMPLE:BMDCS, DILUTION:NA
Zhang LY,Jin QQ,Hölscher C,Li L.Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model. Neural Regen Res 2021;16:1660-70
AR1183 usage in article: APP:WB, SAMPLE:SN TISSUE, DILUTION:NA
Zhao H,Li R,Wang X,Lu X,Hu M,Zhang J,Zhao X,Song X,Liu Y. The role of apatinib combined with paclitaxel (aluminum binding type) in platinum-resistant ovarian cancer. J Ovarian Res.2020 Sep 21;13(1):113. doi:10.1186/s13048-020-00719-3.PMID: 32958014;PMCID:
AR1183 usage in article: APP:WB, SAMPLE:SKOV-3/DDP CELL, DILUTION:NA
Su Q,Li T,He PF,Lu XC,Yu Q,Gao QC,Wang ZJ,Wu MN,Yang D,Qi JS.Trichostatin A ameliorates Alzheimer‘s disease-related pathology and cognitive deficits by increasing albumin expression and Aβ clearance in APP/PS1 mice.Alzheimers Res Ther.2021 Jan 4;13(
AR1183 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:NA
Tang XL,Wang X,Fang G,Zhao YL,Yan J,Zhou Z,Sun R,Luo AL,Li SY.Resveratrol ameliorates sevoflurane-induced cognitive impairment by activating the SIRT1/NF-κB pathway in neonatal mice.J Nutr Biochem.2020 Dec 31:108579.doi:10.1016/j.jnutbio.2020.108579.Epub
AR1183 usage in article: APP:PROTEIN QUANTIFICATION, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:NA
Cosmosiin Increases ADAM10 Expression via Mechanisms Involving 5?UTR and PI3K Signaling
?7 Nicotinic acetylcholine receptor-mediated anti-inflammatory effect in a chronic migraine rat model via the attenuation of glial cell activation
Effect of dexmedetomidine, midazolam, and propofol on lipopolysaccharide-stimulated dendritic cells
Overendocytosis of superparamagnetic iron oxide particles increases apoptosis and triggers autophagic cell death in human osteosarcoma cell under a spinning magnetic field
Chronic sciatic nerve compression induces fibrosis in dorsal root ganglia
Liu B, Liu C, Cong W, Li N, Zhou N, Tang Y, Wei C, Bai H, Zhang Y, Xiao J. Skelet Muscle. 2017 May 11;7(1):8. doi: 10.1186/s13395-017-0126-x. Retinoid acid-induced microRNA-31-5p suppresses myogenic proliferation and differentiation by targeting C...
Huang JM, Bao Y, Xiang W, Jing XZ, Guo JC, Yao XD, Wang R, Guo FJ. Evid Based Complement Alternat Med. 2017;2017:8085325. doi: 10.1155/2017/8085325. Epub 2017 Dec 27. Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal S...
Peng Z, Wang Y, Fan J, Lin X, Liu C, Xu Y, Ji W, Yan C, Su C. Sci Rep. 2017 Jan 24;7:41254. doi: 10.1038/srep41254. Costunolide and dehydrocostuslactone combination treatment inhibit breast cancer by inducing cell cycle arrest and apoptosis throug...
Wei Z, Junhong G, Xiaoyuan N, Jie W, Zhaojun W, Meina W, Wei Y, Jun Z, Jinshun Q. Biomed Res Int. 2017;2017:1238687. doi: 10.1155/2017/1238687. Epub 2017 Sep 18. Citalopram Ameliorates Impairments in Spatial Memory and Synaptic Plasticity in Femal...
Liu D, Zhou Y, Peng Y, Su P, Li Z, Xu Q, Tu Y, Tian X, Yang H, Wu Z, Mei W, Gao F. Front Mol Neurosci. 2018 Mar 6;11:72. doi: 10.3389/fnmol.2018.00072. eCollection 2018. Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of...
Wang P, Mao Z, Pan Q, Lu R, Huang X, Shang X, Zhang R, You H. Int J Mol Med. 2018 Apr;41(4):2117-2127. doi: 10.3892/ijmm.2018.3410. Epub 2018 Jan 22. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabol...
Liang, Y., Song, D.Z., Liang, S., Zhang, Z.F., Gao, L.X., & Fan, X.H. (2017). The hemagglutinin-neuramidinase protein of Newcastle disease virus upregulates expression of the TRAIL gene in murine natural killer cells through the activation of Syk ...
He Ql, Chen Y, Qin J, Mo Sl, Wei M, Zhang Jj, Li Mn, Zou Xn, Zhou Sf, Chen Xw, Sun Lb. Med Sci Monit. 2012 Jun;18(6):Br229-36. Osthole, A Herbal Compound, Alleviates Nucleus Pulposus-Evoked Nociceptive Responses Through The Suppression Of Overexpr...
Have you used Broad Spectrum Phosphatase Inhibitor Cocktail?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Broad Spectrum Phosphatase Inhibitor Cocktail
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question