Broad Spectrum Phosphatase Inhibitor Cocktail

Broad Spectrum Phosphatase Inhibitor Cocktail, For inhibiting Protein tyrosine phosphatase, acidic and alkaline phosphatase; Serine/threonine phosphatase, acidic and alkaline phosphatase; PP1and PP2A. Cited in 26 publication(s).

Product Info Summary

SKU AR1183
Product nameBroad Spectrum Phosphatase Inhibitor Cocktail
Pack size 1 mL
Applications WB sample preparation, protein purification, Co-IP, protein kinase activity assay experiments

Overview

Form Supplied 100X concentrated stock solution
Physical State Liquid, colorless to light yellow
Pack Size 1 mL
Content Proprietary mix of: Sodium fluoride, Sodium orthavanadium, Imidazole, Sodium molybdate, Sodium sulphate, Sodium pyrophosphate, B-phosphoric acid glycerol
Recommended working concentration 100-fold dilution in tissue lysis buffer;
Use 10 µl of the Phosphatase Inhibitor Cocktail solution to inhibit dephosphorylation of proteins for 1 ml of lysate
Storage Upon receipt store at -20°C.
It is stable at -20°C for one year. It is stable at 4°C for two months. Product is shipped on ice.
Equivalent Thermofisher (Product No.78428, A32957); Millipore Sigma (Product No. P5726)
Target Specificity Tyrosine phosphatase, acidic and alkaline phosphatase; Serine/threonine phosphatase, histidine phosphatase, etc.

BioChemical Information

Inhibitor Inhibition Specificity
Sodium fluoride Ser/Thr and acid phosphatases
Sodium orthavanadium ATPases
Tyr and alkaline phosphatases
Imidazole Alkaline phosphatases
Sodium molybdate Acid and phosphoprotein phosphatases
Sodium tartrate Acid phosphatase
Sodium pyrophosphate Ser/Thr phosphatases
B-phosphoric acid glycerol Ser/Thr phosphatases

Assay Principle

Broad Spectrum Phosphatase Inhibitor Cocktail is a Western blot related concentrated stock solution reagent containing a mixture of different phosphatase inhibitors that is to be added to cell lysis buffer to protect native phosphoproteins from dephosphorylation during proteins purification and sample preparation used in WB, Co-IP, ChIP, and protein kinase assays.

Properties

Description Broad Spectrum Phosphatase Inhibitor Cocktail is a Western blot related concentrated stock solution reagent containing a mixture of six phosphatase inhibitors that is to be added to cell lysis buffer to protect native phosphoproteins from dephosphorylation during proteins purification and sample preparation used in WB, Co-IP, ChIP, and protein kinase assays.
Cite This Product Broad Spectrum Phosphatase Inhibitor Cocktail (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1183)
Application WB sample preparation, protein purification, Co-IP, protein kinase activity assay experiments
Our Boster Guarantee covers the use of this product in the above tested applications.
Reagent Type Western Blotting related reagent; Inhibitors
Usage To preserve phosphorylation state and protein functionality following cell lysis
Target Sample Cell lysis extracts
Compatibility with assays Compatible with IEF/2D studies;
MS-compatible: not contain AEBSF;
Compatible with immobilized metal chelate affinity chromatography and 2D gel electrophoresis inhibitor components dialysis removal or desalting;

Usage and Handling

The product is supplied as a 100X concentrated stock solution in a liquid format for improved accuracy, solubility, and ease of use in comparison to traditional tablets. Since phosphatase levels may vary among cell and tissue types, it may be necessary to increase the concentration of inhibitors.

Background

Phosphatase inhibitors are used when phosphorylation (activation) states of target proteins need to be studied and the phosphorylated residues of interest must remain intact. They are chemicals that aid in the extraction of intact proteins in their native modification state by inhibiting endogenous phosphatases that would otherwise dephosphorylate the proteins present in cell lysates and tissue extracts. Broad Spectrum Phosphatase Inhibitor Cocktail contains individual components with specific inhibitory properties to provide an all-around protection of the protein phosphorylation state. The six phosphatase inhibitors included in this mixture target a broad spectrum phosphatase categories.
Dynamic protein phosphorylation is a key cellular signaling mechanism for cell processes regulation. When tissues are lysed to make whole cell extracts, the loss of natural compartmentalization causes normal regulation of cellular signaling to get distorted, and resident cell phosphatases within the cell extract are free to disorderly dephosphorylate proteins. The usual consequence of this unregulated state is biologically meaningless representation of protein activities (i.e. phosphorylation status) and false negative staining in anti-phosphoprotein immunostaining analyses. The addition of phosphatase inhibitors to the cell lysis buffer aids in the preservation of phosphorylated residues at the time of cell disruption.

Product Images

AR1183 has been cited in 26 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Lin X,Bai D,Wei Z,Zhang Y,Huang Y,Deng H,Huang X.Curcumin attenuates oxidative stress in RAW264.7 cells by increasing the activity of antioxidant enzymes and activating the Nrf2-Keap1 pathway.PLoS One.2019 May 21;14(5):e0216711.doi:10.1371/journal.pone.0216711.PMID:31112588;PMCID:PMC6528975.
Species: Mouse
AR1183 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA

Ren Y,Jiang J,Jiang W,Zhou X,Lu W,Wang J,Luo Y.Spata2 Knockdown Exacerbates Brain Inflammation via NF-κB/P38MAPK Signaling and NLRP3 Inflammasome Activation in Cerebral Ischemia/Reperfusion Rats.Neurochem Res.2021 Jun 1.doi:10.1007/s11064-021-03360-8.Epub ahead of print.PMID:34075523.
Species: Rat
AR1183 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA

Zhang T,Liu C,Yu Y,Geng J,Meng Q,Xu S,Zhou F,Chen Y,Jin S,Shen J,Pan B,Meng F,Liu F. TBL1XR1 is involved in c-Met-mediated tumorigenesis of human nonsmall cell lung cancer.Cancer Gene Ther.2020 Apr;27(3-4):136-146.doi:10.1038/s41417-019-0111-0.Epub 2019 Jun 27.PMID:31243347.
Species: Human
AR1183 usage in article: APP:WB, SAMPLE:NSCLC CELL, DILUTION:NA

Wang, X.,Wang, J.,Huang, G.,Li, Y.,& Guo, S. (2021). miR‑320a‑3P alleviates the epithelial‑mesenchymal transition of A549 cells by activation of STAT3/SMAD3 signaling in a pulmonary fibrosis model. Molecular Medicine Reports,23,357.https://doi.org/10.3892/mmr.2021.11996
Species: Human
AR1183 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:NA

Guo J,Ren R,Yao X,Ye Y,Sun K,Lin J,Wang G, Guo F,Xiao J,Xu T.PKM2 suppresses osteogenesis and facilitates adipogenesis by regulating β-catenin signaling and mitochondrial fusion and fission.Aging (Albany NY).2020 Feb 25;12(4):3976-3992.doi:10. 18632/aging
Species: Mouse
AR1183 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:NA

Chen R,Guo S,Yang C,Sun L,Zong B,Li K,Liu L,Tu G,Liu M,Liu S.Although c‑MYC contributes to tamoxifen resistance, it improves cisplatin sensitivity in ER‑positive breast cancer.Int J Oncol.2020 Apr;56(4):932-944.doi:10.3892/ijo.2020.4987.Epub 2020 Feb 14.P
Species: Human

Chu X,Yu T,Huang X,Xi Y,Ni B,Zhang R,You H.Tomatidine suppresses inflammation in primary articular chondrocytes and attenuates cartilage degradation in osteoarthritic rats.Aging (Albany NY).2020 Jul 5;12(13):12799-12811.doi:10.18632/aging.103222.Epub 2020
Species: Rat
AR1183 usage in article: APP:WB, SAMPLE:PRIMARY CHONDROCYTES, DILUTION:NA

Zhang R,Ji J,Zhou X,Li R.Irisin Pretreatment Protects Kidneys against Acute Kidney Injury Induced by Ischemia/Reperfusion via Upregulating the Expression of Uncoupling Protein 2. Biomed Res Int.2020 Aug 31;2020:6537371.doi:10.1155/2020/6537371.PMID:329349
Species: Human,Mouse
AR1183 usage in article: APP:WB, SAMPLE:HK-2 CELL AND KIDNEY TISSUE, DILUTION:NA

Wang C,Sun Z,Zhao C,Zhang Z,Wang H,Liu Y,Guo Y,Zhang B,Gu L,Yu Y,Hu Y,Wu J.Maintaining manganese in tumor to activate cGAS-STING pathway evokes a robust abscopal anti-tumor effect.J Control Release.2021 Feb 2:S0168-3659(21)00045-6.doi:10.1016/j. jconrel.2
Species: Mouse
AR1183 usage in article: APP:WB, SAMPLE:BMDCS, DILUTION:NA

Zhang LY,Jin QQ,Hölscher C,Li L.Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model. Neural Regen Res 2021;16:1660-70
Species: Human,Rat
AR1183 usage in article: APP:WB, SAMPLE:SN TISSUE, DILUTION:NA

Zhao H,Li R,Wang X,Lu X,Hu M,Zhang J,Zhao X,Song X,Liu Y. The role of apatinib combined with paclitaxel (aluminum binding type) in platinum-resistant ovarian cancer. J Ovarian Res.2020 Sep 21;13(1):113. doi:10.1186/s13048-020-00719-3.PMID: 32958014;PMCID:
Species: Human,Mouse
AR1183 usage in article: APP:WB, SAMPLE:SKOV-3/DDP CELL, DILUTION:NA

Su Q,Li T,He PF,Lu XC,Yu Q,Gao QC,Wang ZJ,Wu MN,Yang D,Qi JS.Trichostatin A ameliorates Alzheimer‘s disease-related pathology and cognitive deficits by increasing albumin expression and Aβ clearance in APP/PS1 mice.Alzheimers Res Ther.2021 Jan 4;13(
Species: Mouse
AR1183 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:NA

Tang XL,Wang X,Fang G,Zhao YL,Yan J,Zhou Z,Sun R,Luo AL,Li SY.Resveratrol ameliorates sevoflurane-induced cognitive impairment by activating the SIRT1/NF-κB pathway in neonatal mice.J Nutr Biochem.2020 Dec 31:108579.doi:10.1016/j.jnutbio.2020.108579.Epub
Species: Mouse
AR1183 usage in article: APP:PROTEIN QUANTIFICATION, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:NA

Cosmosiin Increases ADAM10 Expression via Mechanisms Involving 5?UTR and PI3K Signaling

?7 Nicotinic acetylcholine receptor-mediated anti-inflammatory effect in a chronic migraine rat model via the attenuation of glial cell activation

Effect of dexmedetomidine, midazolam, and propofol on lipopolysaccharide-stimulated dendritic cells

Overendocytosis of superparamagnetic iron oxide particles increases apoptosis and triggers autophagic cell death in human osteosarcoma cell under a spinning magnetic field

Chronic sciatic nerve compression induces fibrosis in dorsal root ganglia

Liu B, Liu C, Cong W, Li N, Zhou N, Tang Y, Wei C, Bai H, Zhang Y, Xiao J. Skelet Muscle. 2017 May 11;7(1):8. doi: 10.1186/s13395-017-0126-x. Retinoid acid-induced microRNA-31-5p suppresses myogenic proliferation and differentiation by targeting C...

Huang JM, Bao Y, Xiang W, Jing XZ, Guo JC, Yao XD, Wang R, Guo FJ. Evid Based Complement Alternat Med. 2017;2017:8085325. doi: 10.1155/2017/8085325. Epub 2017 Dec 27. Icariin Regulates the Bidirectional Differentiation of Bone Marrow Mesenchymal S...

Peng Z, Wang Y, Fan J, Lin X, Liu C, Xu Y, Ji W, Yan C, Su C. Sci Rep. 2017 Jan 24;7:41254. doi: 10.1038/srep41254. Costunolide and dehydrocostuslactone combination treatment inhibit breast cancer by inducing cell cycle arrest and apoptosis throug...

Wei Z, Junhong G, Xiaoyuan N, Jie W, Zhaojun W, Meina W, Wei Y, Jun Z, Jinshun Q. Biomed Res Int. 2017;2017:1238687. doi: 10.1155/2017/1238687. Epub 2017 Sep 18. Citalopram Ameliorates Impairments in Spatial Memory and Synaptic Plasticity in Femal...

Liu D, Zhou Y, Peng Y, Su P, Li Z, Xu Q, Tu Y, Tian X, Yang H, Wu Z, Mei W, Gao F. Front Mol Neurosci. 2018 Mar 6;11:72. doi: 10.3389/fnmol.2018.00072. eCollection 2018. Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of...

Wang P, Mao Z, Pan Q, Lu R, Huang X, Shang X, Zhang R, You H. Int J Mol Med. 2018 Apr;41(4):2117-2127. doi: 10.3892/ijmm.2018.3410. Epub 2018 Jan 22. Histone deacetylase-4 and histone deacetylase-8 regulate interleukin-1β-induced cartilage catabol...

Liang, Y., Song, D.Z., Liang, S., Zhang, Z.F., Gao, L.X., & Fan, X.H. (2017). The hemagglutinin-neuramidinase protein of Newcastle disease virus upregulates expression of the TRAIL gene in murine natural killer cells through the activation of Syk ...

He Ql, Chen Y, Qin J, Mo Sl, Wei M, Zhang Jj, Li Mn, Zou Xn, Zhou Sf, Chen Xw, Sun Lb. Med Sci Monit. 2012 Jun;18(6):Br229-36. Osthole, A Herbal Compound, Alleviates Nucleus Pulposus-Evoked Nociceptive Responses Through The Suppression Of Overexpr...

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