Product Info Summary
SKU: | PROTQ99ZW2 |
---|---|
Size: | 50ug, 100ug, 500ug |
Source: | Escherichia coli |
Product info
Product Name
CAS9 CRISPR-Associated Protein-9 Nuclease S. Pyogenes Recombinant Protein
SKU/Catalog Number
PROTQ99ZW2
Size
50ug, 100ug, 500ug
Description
Recombinant Cas9-NLS produced in E.coli, comprises the entire Cas9 protein sequence (1368 amino acids) joined to a proprietary nuclear localization sequence (NLS) and a 6xHis tag at the C-terminus, having a total of 1414 amino acids. The protein has an apparent molecular weight of 163kDa.
Storage & Handling
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Cite This Product
CAS9 CRISPR-Associated Protein-9 Nuclease S. Pyogenes Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ99ZW2)
Form
Sterile Filtered colorless solution.
Formulation
The CAS9 solution contains 10mM Tris-HCl, 300mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.
Purity
Greater than 90% as determined by SDS-PAGE.
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