CAS9 CRISPR-Associated Protein-9 Nuclease S. Pyogenes Recombinant Protein

Recombinant Cas9-NLS produced in E.coli, comprises the entire Cas9 protein sequence (1368 amino acids) joined to a proprietary nuclear localization sequence (NLS) and a 6xHis tag at the C-terminus, having a total of 1414 amino acids. The protein has an apparent molecular weight of 163kDa.

Product Info Summary

SKU: PROTQ99ZW2
Size: 50ug, 100ug, 500ug
Origin Species: Mouse
Source: E. Coli
Application: Cell Culture

Product Name

CAS9 CRISPR-Associated Protein-9 Nuclease S. Pyogenes Recombinant Protein

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SKU/Catalog Number

PROTQ99ZW2

Size

50ug, 100ug, 500ug

Form

Sterile Filtered colorless solution.

Description

Recombinant Cas9-NLS produced in E.coli, comprises the entire Cas9 protein sequence (1368 amino acids) joined to a proprietary nuclear localization sequence (NLS) and a 6xHis tag at the C-terminus, having a total of 1414 amino acids. The protein has an apparent molecular weight of 163kDa.

Storage & Handling

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Cite This Product

CAS9 CRISPR-Associated Protein-9 Nuclease S. Pyogenes Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ99ZW2)

Formulation

The CAS9 solution contains 10mM Tris-HCl, 300mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.

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Validation Images & Assay Conditions

Gene/Protein Information For cas9 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q99ZW2

Gene ID

Gene Name

cas9

Full Name

Weight

Alternative Names

CRISPR-associated endonuclease Cas9/Csn1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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