|Form||Dry-blend powder, sufficient to make 500mL of TBS Blocking Buffer|
|Application||It is recommended for use with all chemiluminescent membranebased detection kits and immunoassay applications.|
|Product Name||Casein-based TBS Blocking Buffer Pack|
|Form||Dry-blend powder, sufficient to make 500mL of PBS Blocking Buffer|
|Buffer Contents||50mM Tris, 0.15M NaCl, 1% casein, pH 7.2|
|Working concentration||10mL for each membrane of 5cm×8.5cm|
|Storage||Store at 4°C for one year.|
|Reagent Type||Blocking Reagent|
|Equivalent||Thermofisher (Product No. 37583, 37532)|
|Cite This Product||Casein-based TBS Blocking Buffer Pack (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0183)|
|Application||It is recommended for use with all chemiluminescent membrane-based detection kits and immunoassay applications.
*Our Boster Guarantee covers the use of this product in the above tested applications.
Boster’s casein-based TBS Blocking Buffer Pack is a highly purified casein-based reagent. It provides superior blocking compared to both dried milk and BSA. Unlike other casein-based blocking reagents, this reagent is essentially biotin-free. This reagent is tested in assays using Tropix substrates and alkaline phosphatase conjugates, and is useful as a blocking reagent in membrane-based and immunoassay applications.
• Purified casein—single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
• Easy to use—1% casein solutions are ready to use; can be diluted further as needed
• Safe—stable, thimerosal-free formulations
Completely dissolve the dry powder with 40°C-50°C alkaline solution (adjust pH to 11 with NaOH) to make a total volume of 500mL. After dissolution, adjust pH to 7.4 and mix thoroughly.
Casein-based TBS Blocking Buffer Pack Images
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