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MIP-3 alpha/CCL20 ELISA Kit

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Human MIP-3 Alpha/CCL20 PicoKine™ ELISA Kit EK0453

$380.00 Publications: 3

Pack Size:	96wells/kit, with removable strips.
Validated Species:	Human
Application:	ELISA
Sensitivity:	<1pg/ml
Sample Type:	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Availability:	Ships next business day

Human MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve

Mouse MIP-3 Alpha/CCL20 PicoKine™ ELISA Kit EK0454

$369.00 Publications: 1

Pack Size:	96wells/kit, with removable strips.
Validated Species:	Mouse
Application:	ELISA
Sensitivity:	<1pg/ml
Sample Type:	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Availability:	Ships next business day

Mouse MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve

Rat MIP-3 Alpha/CCL20 PicoKine™ ELISA Kit EK0455

$390.00 Publications: 1

Pack Size:	96wells/kit, with removable strips.
Validated Species:	Rat
Application:	ELISA
Sensitivity:	<1pg/ml
Sample Type:	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Availability:	Ships next business day

Rat MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve

Anti-MIP-3 Alpha/CCL20 Picoband™ Antibody A00748-2

$240.00

Pack Size:	100ug/vial
Validated Species:	Mouse, Rat
Clonality:	Polyclonal
Application:	ELISA, WB

Availability:	Ships in 5-7 business days

Figure 1. Western blot analysis of MIP-3 Alpha/CCL20 using anti-MIP-3 Alpha/CCL20 antibody (A00748-2). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat testis tissue lysates,<br>Lane 2: mouse testis tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIP-3 Alpha/CCL20 antigen affinity purified polyclonal antibody (Catalog # A00748-2) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIP-3 Alpha/CCL20 at approximately 14KD. The expected band size for MIP-3 Alpha/CCL20 is at 14KD.

Anti-MIP-3 alpha Antibody A00748

$200.00

Pack Size:	100μl
Validated Species:	Human, Mouse
Predicted Species:	Bovine, Pig
Clonality:	Polyclonal
Application:	IHC

Availability:	Ships in 5-7 business days

Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200.

Anti-MIP-3 alpha Antibody Biotin Conjugated B00748-1

$329.00

Pack Size:	100μg
Validated Species:	Human
Conjugate:	Biotin
Clonality:	Polyclonal
Application:	WB

Availability:	Ships in 5-7 business days

Western Blot of Biotin Conjugated Rabbit Anti-Human MIP-3 alpha polyclonal antibody (Catalog # B00748-1) at 1:1000. Lane 1: Human MIP-3 alpha. Lane 2: None. Load: 50 ng per lane. Predicted/Observed size: 10 kDa, 10 kDa for Human MIP-3 alpha. Other band(s): None. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).