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Anti-TP53INP2 Antibody A07881-1

$299.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100 ul
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships in 5-7 business days

Figure 1. Western blot analysis of TP53INP2 using anti-TP53INP2 antibody (A07881-1). <br><br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Sample: Human A549 Whole Cell<br><br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53INP2 antigen affinity purified polyclonal antibody (Catalog # A07881-1) at 1ug/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53INP2. The expected band size for TP53INP2 is at 23.9KD.

Figure 2. Western blot analysis of TP53INP2 using anti-TP53INP2 antibody (A07881-1). <br><br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Sample: Human heart<br><br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53INP2 antigen affinity purified polyclonal antibody (Catalog # A07881-1) at 0.2-1 ug/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53INP2. The expected band size for TP53INP2 is at 23.9KD.

Anti-TP53INP2 Antibody A30521

$299.00

Product Category:	Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100ug/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	IF, IHC

Availability:	Ships in 5-7 business days

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