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Anti-HLF Picoband™ Antibody A01284-1

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships in 7-10 business days

Figure 1. Western blot analysis of HLF using anti-HLF antibody (A01284-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human placenta tissue lysates,<br>Lane 2: rat brain tissue lysates,<br>Lane 3: rat liver tissue lysates,<br>Lane 4: rat kidney tissue lysates,<br>Lane 5: rat lung whole cell lysates,<br>Lane 6: mouse brain tissue lysates,<br>Lane 7: mouse liver tissue lysates,<br>Lane 8: mouse kidney tissue lysates,<br>Lane 9: mouse lung tissue lysates. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLF antigen affinity purified polyclonal antibody (Catalog # A01284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLF at approximately 33KD. The expected band size for HLF is at 33KD.

Anti-Mad/MXD1 Picoband™ Antibody A06485-1

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	ELISA, WB

Availability:	Ships next business day

Figure 1. Western blot analysis of Mad using anti-Mad antibody (A06485-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat testis tissue lysates,<br>Lane 2: mouse ovary tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mad antigen affinity purified polyclonal antibody (Catalog # A06485-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mad at approximately 28KD. The expected band size for Mad is at 25KD.

Anti-MATH1/HATH1/ATOH1 Picoband™ Antibody A02207-1

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100ug/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships in 7-10 business days

<b>Figure 1. Western blot analysis of MATH1/HATH1 using anti-MATH1/HATH1 antibody (A02207-1).
</b><br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysate,<br>Lane 2: mouse brain tissue lysate
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MATH1/HATH1 antigen affinity purified polyclonal antibody (Catalog # A02207-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MATH1/HATH1 at approximately 38KD. The expected band size for MATH1/HATH1 is at 38KD.

Anti-NPAS2 Picoband™ Antibody A02688-1

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100ug/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships in 7-10 business days

<b>Figure 1. Western blot analysis of NPAS2 using anti-NPAS2 antibody (A02688-1).
</b><br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysate,<br>Lane 2: mouse brain tissue lysate,<br>Lane 3: human COLO-320 whole cell lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPAS2 antigen affinity purified polyclonal antibody (Catalog # A02688-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPAS2 at approximately 92KD. The expected band size for NPAS2 is at 92KD.

Anti-JunD Picoband™ Antibody A05609-1

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships in 7-10 business days

Figure 1. Western blot analysis of JunD using anti-JunD antibody (A05609-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat spleen tissue lysates,<br>Lane 2: rat lung tissue lysates,<br>Lane 3: rat testis tissue lysates,<br>Lane 4: mouse spleen tissue lysates,<br>Lane 5: mouse lung tissue lysates,<br>Lane 6: mouse testis tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JunD antigen affinity purified polyclonal antibody (Catalog # A05609-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JunD at approximately 35KD. The expected band size for JunD is at 35KD.

Figure 2. Western blot analysis of JunD using anti-JunD antibody (A05609-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human placenta tissue lysates,<br>Lane 2: human Jurkat whole cell lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JunD antigen affinity purified polyclonal antibody (Catalog # A05609-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JunD at approximately 35KD. The expected band size for JunD is at 35KD.

Anti-Myf5 Picoband™ Antibody A04040-1

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	ELISA, WB

Availability:	Ships next business day

Figure 1. Western blot analysis of Myf5 using anti-Myf5 antibody (A04040-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human SW620 cell lysates, <br>Lane 2: mouse testis tissue lysates.<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myf5 antigen affinity purified polyclonal antibody (Catalog # A04040-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Myf5 at approximately 28KD. The expected band size for Myf5 is at 28KD.

Anti-JunB Picoband™ Antibody PB9226

$280.00

Product Category:	Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	IHC, WB

Availability:	Ships next business day

Anti-JunB antibody, PB9226, Western blotting<br>All lanes: Anti JunB (PB9226) at 0.5ug/ml<br>WB: Recombinant Human JunB Protein 0.5ng<br>Predicted bind size: 19KD<br>Observed bind size: 19KD

Anti-JunB antibody, PB9226, Western blotting<br>All lanes: Anti JunB (PB9226) at 0.5ug/ml<br>Lane 1: Human Placenta Tissue Lysate at 50ug<br>Lane 2: Rat Spleen Tissue Lysate at 50ug<br>Lane 3: JURKAT Whole Cell Lysate at 40ug<br>Lane 4: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 36KD<br>Observed bind size: 36KD

Anti-JunB antibody, PB9226, IHC(P)<br>IHC(P): Mouse Brain Tissue

Anti-JunB antibody, PB9226, IHC(P)<br>IHC(P): Rat Brain Tissue

Anti-JunB antibody, PB9226, IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue

Anti-MAX Picoband™ Antibody A03239

$280.00 Publications: 1

Product Category:	Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	ELISA, IHC, WB

Availability:	Ships next business day

Figure 1. Western blot analysis of MAX using anti-MAX antibody (A03239).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat kidney tissue lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAX antigen affinity purified polyclonal antibody (Catalog # A03239) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAX at approximately 18KD. The expected band size for MAX is at 18KD.

Figure 2. IHC analysis of MAX using anti-MAX antibody (A03239).
<br>MAX was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAX Antibody (A03239) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>

Figure 3. IHC analysis of MAX using anti-MAX antibody (A03239).
<br>MAX was detected in paraffin-embedded section of mouse cardiac muscle tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAX Antibody (A03239) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of MAX using anti-MAX antibody (A03239).
<br>MAX was detected in paraffin-embedded section of rat cardiac muscle tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAX Antibody (A03239) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Anti-HIF-1-alpha/HIF1A Picoband™ Antibody PB9253

$280.00 Publications: 39

Product Category:	Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	IHC, WB

Availability:	Ships next business day

Figure 1. Western blot analysis of HIF-1-alpha/HIF1A using anti-HIF-1-alpha/HIF1A antibody (PB9253). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates, <br> Lane 2: human Caco-2 whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-1-alpha/HIF1A antigen affinity purified polyclonal antibody (Catalog # PB9253) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF-1-alpha/HIF1A at approximately 115-120KD. The expected band size for HIF-1-alpha/HIF1A is at 97KD.

Figure 2. IHC analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253). <br> HIF 1 alpha was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HIF 1 alpha Antibody (PB9253) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253). <br> HIF 1 alpha was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HIF 1 alpha Antibody (PB9253) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 4. IHC analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253). <br> HIF 1 alpha was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HIF 1 alpha Antibody (PB9253) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-PER1 Picoband™ Antibody A00876

$280.00

Product Category:	Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	ELISA, IHC, WB

Availability:	Ships in 7-10 business days

Figure 1. Western blot analysis of PER1 using anti-PER1 antibody (A00876).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human PANC cell lysate,<br>Lane 2: human MCF-7 cell lysate,<br>Lane 3: human COLO-320 cell lysate,<br>Lane 4: rat heart tissue lysate,<br>Lane 5: mouse brain tissue lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PER1 antigen affinity purified polyclonal antibody (Catalog # A00876) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PER1 at approximately 200KD. The expected band size for PER1 is at 136KD.

Figure 2. IHC analysis of PER1 using anti-PER1 antibody (A00876).
<br>PER1 was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PER1 Antibody (A00876) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>