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Anti-AP2M1 Picoband™ Antibody A06179

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships in 7-10 business days

Figure 1. Western blot analysis of AP2M1 using anti- AP2M1 antibody (A06179). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U2OS whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: rat kidney tissue lysates, <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- AP2M1 antigen affinity purified polyclonal antibody (Catalog # A06179) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2M1 at approximately 50KD. The expected band size for AP2M1 is at 50KD.

Anti-AP2B1 Picoband™ Antibody A06544

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships in 7-10 business days

Western blot analysis of AP2B1 expression in rat testis extract (lane 1), mouse brain extract (lane 2) and MCF-7 whole cell lysates (lane 3). AP2B1 at 104KD was detected using rabbit anti- AP2B1 Antigen Affinity purified polyclonal antibody (Catalog # A06544) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Anti-Flotillin 2/FLOT2 Picoband™ Antibody PB9623

$280.00

Product Category:	Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	IHC, WB

Availability:	Ships next business day

Anti- Flotillin 2 Picoband antibody, PB9623, Western blotting<br>All lanes: Anti Flotillin 2 (PB9623) at 0.5ug/ml<br>
Lane 1: Rat Liver Tissue Lysate at 50ug<br>
Lane 2: Rat Lung Tissue Lysate at 50ug<br>
Lane 3: Mouse Liver Tissue Lysate at 50ug<br>
Lane 4: Mouse Lung Tissue Lysate at 50ug<br>
Lane 5: Mouse Testis Tissue Lysate at 50ug<br>
Lane 6: Human Placenta Tissue Lysate at 50ug<br>
Lane 7: A375 Whole Cell Lysate at 40ug<br>
Lane 8: HELA Whole Cell Lysate at 40ug<br>
Lane 9: 22RV1 Whole Cell Lysate at 40ug<br>
Lane 10: NIH3T3 Whole Cell Lysate at 40ug<br>
Predicted bind size: 47KD<br>
Observed bind size: 47KD

Anti- Flotillin 2 Picoband antibody, PB9623, IHC(P)<br>IHC(P): Mouse Intestine Tissue

Anti- Flotillin 2 Picoband antibody, PB9623, IHC(P)<br>IHC(P): Rat Kidney Tissue

Anti- Flotillin 2 Picoband antibody, PB9623, IHC(P)<br>IHC(P): Human Mammary Cancer Tissue

Figure 5. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623).<br>
Flotillin 2 was detected in frozen section of mouse kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 6. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623).<br>
Flotillin 2 was detected in frozen section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 7. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623).<br>
Flotillin 2 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-COPE Picoband™ Antibody A04544

$280.00

Product Category:	Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100ug/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB

Availability:	Ships next business day

<b>Figure 1. Western blot analysis of COPE using anti-COPE antibody (A04544). </b><br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat stomach tissue lysate,<br>Lane 2: rat small intestine tissue lysate,<br>Lane 3: rat pancreas tissue lysate,<br>Lane 4: mouse stomach tissue lysate,<br>Lane 5: mouse small intestine tissue lysate,<br>Lane 6: mouse pancreas tissue lysate,<br>Lane 7: human MCF-7 whole Cell lysatem,<br>Lane 8: human Hela whole Cell lysate,<br>Lane 9: human 22RV1 whole Cell lysate. <br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COPE antigen affinity purified polyclonal antibody (Catalog # A04544) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COPE at approximately 34KD. The expected band size for COPE is at 34KD.

<b>Figure 2. IHC analysis of COPE using anti-COPE antibody (A04544).
</b><br>COPE was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-COPE Antibody (A04544) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

<b>Figure 3. IHC analysis of COPE using anti-COPE antibody (A04544).
</b><br>COPE was detected in paraffin-embedded section of mouse pancreas tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-COPE Antibody (A04544) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
</b><br>

<b>Figure 4. IHC analysis of COPE using anti-COPE antibody (A04544).</b><br>COPE was detected in paraffin-embedded section of rat pancreas tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-COPE Antibody (A04544) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>

Figure 5. IF analysis of COPE using anti- COPE antibody (A04544) <br> COPE was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- COPE Antibody (A04544) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Figure 6. Flow Cytometry analysis of HepG2 cells using anti- COPE antibody (A04544). <br> Overlay histogram showing HepG2 cells stained with A04544 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COPE Antibody (A04544,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody M06179

$299.00

Product Category:	Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies
Pack Size:	100ug/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Monoclonal
Clone Number:	GDD-1
Application:	WB

Availability:	Ships in 7-10 business days

Western blot analysis of AP2M1 expression in (1) HEK293 cell lysate; (2) MCF-7 cell lysate (M06179). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2M1 monoclonal antibody (Catalog # M06179) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2M1

Anti-Flotillin 1 Rabbit Monoclonal Antibody M05980

$299.00

Product Category:	Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100ug/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Monoclonal
Clone Number:	BHB-8
Application:	IP, IF, IHC, WB

Availability:	Ships in 7-10 business days

Immunohistochemical analysis of paraffin-embedded human uterus, using Flotillin 1 Antibody (M05980)<br>Flotillin 1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flotillin 1 Antibody (M05980)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>

Western blot analysis of Flotillin 1 expression in (1) HeLa cell lysate; (2) K562 cell lysate (M05980). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flotillin 1 monoclonal antibody (Catalog # M05980) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Flotillin 1

Anti-Flotillin 2 Rabbit Monoclonal Antibody M06107

$299.00

Product Category:	Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies
Pack Size:	100ug/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Monoclonal
Clone Number:	GB-6
Application:	IP, WB

Availability:	Ships in 7-10 business days

Western blot analysis of Flotillin-2 expression in (1) Hela cell lysate;(2) 293 cell lysate (M06107). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLOT2 monoclonal antibody (Catalog # M06107) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLOT2

Anti-Clathrin heavy chain Rabbit Monoclonal Antibody M03134

$299.00

Product Category:	Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100ug/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Monoclonal
Clone Number:	ABDH-3
Application:	WB

Availability:	Ships in 7-10 business days

Western blot analysis of Clathrin heavy chain expression in Hela cell lysate (M03134). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLTC monoclonal antibody (Catalog # M03134) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLTC

Anti-Flotillin 1 Rabbit Monoclonal Antibody M04517

$299.00

Product Category:	Primary Antibodies, Monoclonal Antibodies, Rabbit Monoclonal Antibodies, Polyclonal Antibodies
Pack Size:	100ug/vial
Validated Species:	Human, Mouse
Clonality:	Monoclonal
Clone Number:	BHG-6
Application:	WB

Availability:	Ships in 7-10 business days

Western blot analysis of Flotillin 1 expression in HeLa cell lysate (M04517). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLOT1 monoclonal antibody (Catalog # M04517) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLOT1

Anti-Flotillin 2/FLOT2 Antibody PA2034

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships next business day

Anti-Flotillin 2 antibody, PA2034, Western blotting<br>Lane 1: Rat Lung Tissue Lysate<br>Lane 2: 293T Cell Lysate<br>