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Anti-GSTM1 Picoband™ Antibody (monoclonal, 11F2) M00569

$280.00 Publications: 1

Product Category:	Primary Antibodies, Monoclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Monoclonal
Clone Number:	11F2
Application:	Flow Cytometry, IHC, ICC, WB

Availability:	Ships in 7-10 business days

Figure 1. Flow Cytometry analysis of U20S cells using anti-GSTM1 antibody (M00569). <br>Overlay histogram showing U20S cells stained with M00569 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSTM1 Antibody (M00569,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Figure 2. Flow Cytometry analysis of HELA cells using anti-GSTM1 antibody (M00569). <br>Overlay histogram showing HELA cells stained with M00569 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GSTM1 Antibody (M00569,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Figure 3. IHC analysis of GSTM1 using anti-GSTM1 antibody (M00569). <br>GSTM1 was detected in paraffin-embedded section of rat liver tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-GSTM1 Antibody (M00569) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 4. IHC analysis of GSTM1 using anti-GSTM1 antibody (M00569). <br>GSTM1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-GSTM1 Antibody (M00569) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Figure 5. Western blot analysis of GSTM1 using anti-GSTM1 antibody (M00569). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela, whole cell lysate,<br> Lane 2: human T-47D whole cell lysate,<br> Lane 3: rat brain tissue lysate,<br> Lane 4: rat lung tissue lysate,<br> Lane 5: rat stomach tissue lysate, <br> Lane 6: mouse lung tissue lysate,<br> Lane 7: mouse stomach tissue lysate,<br> Lane 8: mouse kidney tissue lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GSTM1 antigen affinity purified monoclonal antibody (Catalog # M00569) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.

Anti-PON1 Picoband™ Antibody (monoclonal, 9D3) M00516

$280.00

Product Category:	Primary Antibodies, Monoclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100μg/vial
Validated Species:	Mouse, Rat
Clonality:	Monoclonal
Clone Number:	9D3
Application:	ELISA, IHC, WB

Availability:	Ships next business day

Figure 1. Western blot analysis of PON1 using anti-PON1 antibody (M00516). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse liver tissue lysates, <br>Lane 2: mouse liver tissue lysates, <br>Lane 3: rat liver tissue lysates,<br> Lane 4: rat liver tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PON1 antigen affinity purified monoclonal antibody (Catalog # M00516) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

Anti-EPHX2 Picoband™ Antibody A01999-1

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	Flow Cytometry, WB

Availability:	Ships in 7-10 business days

Figure 1. Western blot analysis of EPHX2 using anti-EPHX2 antibody (A01999-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> lane 1: rat lung tissue lysates,<br> lane 2: mouse lung tissue lysates,<br> lane 3: HEPG2 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHX2 antigen affinity purified polyclonal antibody (Catalog # A01999-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHX2 at approximately 62KD. The expected band size for EPHX2 is at 62KD.

Figure 2. Flow Cytometry analysis of PC-3 cells using anti- EPHX2 antibody (A01999-1). <br> Overlay histogram showing PC-3 cells stained with A01999-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPHX2 Antibody (A01999-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Anti-FMO1 Picoband™ Antibody PB9589

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships next business day

Anti- FMO1 Picoband antibody, PB9589, Western blotting<br>
All lanes: Anti FMO1 (PB9589) at 0.5ug/ml<br>
Lane 1: Rat Liver Tissue Lysate at 50ug<br>
Lane 2: Mouse Liver Tissue Lysate at 50ug<br>
Lane 3: Rat Kidney Tissue Lysate at 50ug<br>
Lane 4: Mouse Kidney Tissue Lysate at 50ug<br>
Lane 5: SMMC Whole Cell Lysate at 40ug<br>
Predicted bind size: 60KD<br>
Observed bind size: 60KD

Anti-FMO3 Picoband™ Antibody PB9590

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships next business day

Anti- FMO3 Picoband antibody, PB9590, Western blotting<br>All lanes: Anti FMO3 (PB9590) at 0.5ug/ml<br>
Lane 1: Rat Liver Tissue Lysate at 50ug<br>
Lane 2: Mouse Liver Tissue Lysate at 50ug<br>
Lane 3: SMMC Whole Cell Lysate at 40ug<br>
Predicted bind size: 60KD<br>
Observed bind size: 60KD

Anti-TPMT Picoband™ Antibody A00671-1

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	ELISA, WB

Availability:	Ships next business day

Figure 1. Western blot analysis of TPMT using anti-TPMT antibody (A00671-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: human SGC-7901 whole cell lysates, <br>Lane 2: rat testis tissue lysates,<br>Lane 3: mouse testis tissue lysates.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPMT antigen affinity purified polyclonal antibody (Catalog # A00671-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPMT at approximately 28KD. The expected band size for TPMT is at 28KD.

Anti-GSTM1 Picoband™ Antibody A00569-1

$240.00 Publications: 1

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	Flow Cytometry, WB

Availability:	Ships next business day

Figure 1. Western blot analysis of GSTM1 using anti-GSTM1 antibody (A00569-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat brain tissue lysates,<br>Lane 2: rat lung tissue lysates,<br>Lane 3: mouse stomach tissue lysates,<br>Lane 4: mouse kidney tissue lysates.<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM1 antigen affinity purified polyclonal antibody (Catalog # A00569-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTM1 at approximately 26KD. The expected band size for GSTM1 is at 26KD.

Figure 2. Flow Cytometry analysis of HeLa cells using anti- GSTM1 antibody (A00569-1). <br> Overlay histogram showing HeLa cells stained with A00569-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSTM1 Antibody (A00569-1,1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Anti-ADH4 Picoband™ Antibody PB10030

$240.00

Product Category:	Primary Antibodies, Polyclonal Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	WB

Availability:	Ships in 7-10 business days

Western blot analysis of ADH4 expression in rat liver extract (lane 1), mouse liver extract (lane 2) and HEPG2 whole cell lysates (lane 3). ADH4 at 40KD was detected using rabbit anti- ADH4 Antigen Affinity purified polyclonal antibody (Catalog # PB10030) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Anti-GSTM3 Picoband™ Antibody A03298-1

$280.00

Product Category:	Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	ELISA, IHC, WB

Availability:	Ships next business day

Figure 1. Western blot analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat testis tissue lysate,<br>Lane 2: rat brain tissue lysate,<br>Lane 3: mouse testis tissue lysate,<br>Lane 4: mouse brain tissue lysate,<br>Lane 5: human Hela cell lysate,<br>Lane 6: human MCF-7 cell lysate.
<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM3 antigen affinity purified polyclonal antibody (Catalog # A03298-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTM3 at approximately 26KD. The expected band size for GSTM3 is at 26KD.

Figure 2. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).
<br>GSTM3 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>

Figure 3. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).
<br>GSTM3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>

Figure 4. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).
<br>GSTM3 was detected in paraffin-embedded section of human testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>

Figure 5. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).
<br>GSTM3 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>

Figure 6. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).
<br>GSTM3 was detected in paraffin-embedded section of mouse testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>

Figure 7. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).
<br>GSTM3 was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>

Figure 8. IHC analysis of GSTM3 using anti-GSTM3 antibody (A03298-1).
<br>GSTM3 was detected in paraffin-embedded section of rat testis tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GSTM3 Antibody (A03298-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>

Anti-UGT1A1 Picoband™ Antibody A01865-1

$280.00

Product Category:	Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies
Pack Size:	100μg/vial
Validated Species:	Human, Mouse, Rat
Clonality:	Polyclonal
Application:	ELISA, IHC, WB

Availability:	Ships next business day

Figure 1. Western blot analysis of UGT1A1 using anti-UGT1A1 antibody (A01865-1).
<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
<br>Lane 1: rat liver tissue lysates,<br>Lane 2: rat kidney tissue lysates,<br>Lane 3: mouse liver tissue lysates,<br>Lane 4: mouse kidney tissue lysates.<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UGT1A1 antigen affinity purified polyclonal antibody (Catalog # A01865-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UGT1A1 at approximately 59KD. The expected band size for UGT1A1 is at 59KD.

Figure 2. IHC analysis of UGT1A1 using anti-UGT1A1 antibody (A01865-1).
UGT1A1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UGT1A1 Antibody (A01865-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis of UGT1A1 using anti-UGT1A1 antibody (A01865-1).<br>
UGT1A1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UGT1A1 Antibody (A01865-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 4. IHC analysis of UGT1A1 using anti-UGT1A1 antibody (A01865-1).
<br>UGT1A1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UGT1A1 Antibody (A01865-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 5. IHC analysis of UGT1A1 using anti-UGT1A1 antibody (A01865-1).
<br>UGT1A1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-UGT1A1 Antibody (A01865-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
<br>