Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit

The Claudin 3 (Phospho-Tyr219) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Claudin 3 (Phospho-Tyr219) protein expression profile in cells. The kit can be used for measuring the relative amounts of Claudin 3 (Phospho-Tyr219) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Claudin 3 (Phospho-Tyr219).

Product Info Summary

SKU: EKC2608
Size: 1 kit, containing 2 plates and all necessary reagents
Reactive Species: Human
Application: ELISA

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Product Name

Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit

See all Claudin-3 products

SKU/Catalog Number

EKC2608

Size

1 kit, containing 2 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The Claudin 3 (Phospho-Tyr219) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Claudin 3 (Phospho-Tyr219) protein expression profile in cells. The kit can be used for measuring the relative amounts of Claudin 3 (Phospho-Tyr219) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Claudin 3 (Phospho-Tyr219).

Storage & Handling

4°C/6 Months

Cite This Product

Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2608)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC2608 is reactive to CLDN3 in Human samples

Application Guarantee

EKC2608 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-CLDN3 (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-CLDN3 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Gene/Protein Information For CLDN3 (Source: Uniprot.Org, NCBI)

Uniprot ID

O15551

Gene ID

1365

Gene Name

CLDN3

Full Name

Claudin-3

Weight

23.319kDa

Superfamily

claudin family

Alternative Names

C7orf1; claudin 3; Claudin3; Claudin-3; CLDN3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-R2; CPETR2; CPETR2CPE-receptor 2; HRVP1; Rat ventral prostate.1 protein homolog; RVP1; ventral prostate.1 protein homolog; ventral prostate.1-like protein CLDN3 C7orf1, CPE-R2, CPETR2, HRVP1, RVP1 claudin 3 claudin-3|CPE-R 2|CPE-receptor 2|Clostridium perfringens enterotoxin receptor 2|ventral prostate.1 protein homolog|ventral prostate.1-like protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CLDN3, check out the CLDN3 Infographic

CLDN3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CLDN3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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1 Customer Q&As for Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit

Question

Does Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit stain perineurial cells?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

Indeed, the Claudin 3 (Phospho-Tyr219) Colorimetric Cell-Based ELISA Kit is expressed in perineurial cells.

Boster Scientific Support

Answered: 2020-01-28

$480

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