Cyclin D1 Colorimetric Cell-Based ELISA Kit

CCND1 ELISA kit, cell-based

The Cyclin D1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Cyclin D1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Cyclin D1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Cyclin D1. Cited in 1 publication(s).

Product Info Summary

SKU: EKC1153
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

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Product Name

Cyclin D1 Colorimetric Cell-Based ELISA Kit

See all CCND1 products

SKU/Catalog Number

EKC1153

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The Cyclin D1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Cyclin D1 protein expression profile in cells. The kit can be used for measuring the relative amounts of Cyclin D1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Cyclin D1.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

Cyclin D1 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1153)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1153 is reactive to CCND1 in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1153 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-CCND1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For CCND1 (Source: Uniprot.Org, NCBI)

Gene Name

CCND1

Full Name

G1/S-specific cyclin-D1

Weight

33.729kDa

Superfamily

cyclin family

Alternative Names

B-cell lymphoma 1 protein; BCL-1 oncogene; Bcl-1; BCL1D11S287E; CCND1; cyclin D1 (PRAD1: parathyroid adenomatosis 1); Cyclin D1; Cyl-1; G1/S-specific cyclin D1; G1/S-specific cyclin-D1; PRAD1 oncogene; PRAD1; PRAD1B-cell CLL/lymphoma 1; U21B31 CCND1 BCL1, D11S287E, PRAD1, U21B31 cyclin D1 G1/S-specific cyclin-D1|B-cell CLL/lymphoma 1|B-cell lymphoma 1 protein|BCL-1 oncogene|PRAD1 oncogene

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CCND1, check out the CCND1 Infographic

CCND1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CCND1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EKC1153 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The antitumor activity of umbelliferone in human renal cell carcinoma via regulation of the p110γ catalytic subunit of PI3Kγ Xue Wang 1 2, Shuaishuai Huang 1 2, Xia Xin 1, Yu Ren 1 2, Guobin Weng 1 2, Ping Wang 3 Acta Pharm. 2019 Mar 1;69(1):111-119.

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