Cytochrome c Colorimetric Cell-Based ELISA Kit

Cytochrome c ELISA kit, cell-based

The Cytochrome c Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Cytochrome c protein expression profile in cells. The kit can be used for measuring the relative amounts of Cytochrome c in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Cytochrome c.

Product Info Summary

SKU: EKC1161
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

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Product Name

Cytochrome c Colorimetric Cell-Based ELISA Kit

See all Cytochrome c products

SKU/Catalog Number

EKC1161

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The Cytochrome c Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Cytochrome c protein expression profile in cells. The kit can be used for measuring the relative amounts of Cytochrome c in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Cytochrome c.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

Cytochrome c Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1161)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1161 is reactive to Cycs in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1161 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Cytochrome c Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For Cycs (Source: Uniprot.Org, NCBI)

Gene Name

Cycs

Full Name

Cytochrome c, somatic

Weight

11.605kDa

Superfamily

cytochrome c family

Alternative Names

CYCHCS; CYCS; Cytochrome c; cytochrome c, somatic; THC4 Cycs|cytochrome c, somatic|cytochrome c, somatic

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cycs, check out the Cycs Infographic

Cycs infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cycs: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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3 Customer Q&As for Cytochrome c Colorimetric Cell-Based ELISA Kit

Question

What is your protocol for EKC1161 based on serum test?

Verified customer

Asked: 2020-01-08

Answer

The following is the protocol for Cytochrome c Colorimetric Cell-Based ELISA Kit EKC1161 used on serum: 1) Collect whole blood into a tube without additives. 2) Keep the blood at room temperature for 30 min. 3) Centrifuge at 3,000 rpm and 4°C for 10 min. 4) Assay immediately or aliquot supernatant (serum) and hold at -80°C (Avoid freeze/thaw cycles). Note: Hemolysis should be avoided while collecting serum samples. Those samples that have undergone hemolysis may increase non-specifically staining in HRP-conjugated ELISA assay.

Boster Scientific Support

Answered: 2020-01-09

Question

Can you confirm if EKC1161 and EKC1086 ELISA kits can be used for serum/plasma samples?

Verified customer

Asked: 2019-12-30

Answer

The Cytochrome C Colorimetric Cell-Based ELISA Kit (EKC1161) and Caspase 3 Colorimetric Cell-Based ELISA Kit (EKC1086) are specifically geared towards whole cells (adherent or suspension) seeded to the bottom of the plate. It is not intended to be used with cell lysates or any other types of samples.

Boster Scientific Support

Answered: 2020-01-02

Question

Can frozen tissue extracts be used as a sample for EKC1086 and EKC1161? What should be the weight or volume of sample?

Verified customer

Asked: 2019-06-10

Answer

Our cell-based ELISA kits are meant to be used with cells being plated, grown, then fixed on the wells. They are not meant to be used with cell lysates or tissue homogenates, as they have not been validated for that purpose. We cannot offer any sort of guarantee using it on tissue samples.

Boster Scientific Support

Answered: 2019-06-13

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