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IntroductionDAPI is a blue fluorescent counterstain solution that is effective in cell staining and quantification of DNA content. The fluorescent probe is permeable to the cell and binds to the minor groove of the DNA. The DAPI counterstain solution is compatible with direct or indirect antibody-based detection method. The applications of DAPI counterstain solution include immunofluorescence microscopy, in situ hybridization, flow cytometry, or tissue staining.
|Product Name||DAPI Counterstaining Solution|
|Description||DAPI is a popular blue fluorescent counterstain solution used to stain DNA and nuclear content in fluorescence microscopy and live cell imaging.|
|Cite This Product||DAPI Counterstaining Solution (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1177)|
|Storage||Store at -20°C in dark for one year|
DAPI counterstaining solution popularly used for nuclear stain fluorescent technique for live cell imaging. It gives blue color to the nucleus, which is prominent in comparison to other counterstaining solutions. The DAPI nuclear counterstain solution is a highly pure form of diamidino-2-phenylindole exclusively used to stain DNA and other nuclear content in cellular imaging technique.
DAPI Counterstaining Solution Images
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NIH-3T3 cells were grown in 96 well plates, fixed with paraformaldehyde and permeabilized. Then incubate with Boster DAPI solution for 5 minutes.
Li, M., Wang, P., Zheng, Z., Hu, K., Zhang, M., Guan, X.,…, & Liu, Y. (2017). Japanese encephalitis virus counteracts BST2 restriction via its envelope protein E. Virology, 510, 67-75. Advance online publication. doi: 10.1016/j.virol.2017.07.008
Zhang, B., Wei, K., Li, X., Hu, R., Qiu, J., Zhang, Y.,…, & Zhu, C. (2017). Upregulation of Cdh1 signaling in the hippocampus attenuates brain damage after transient global cerebral ischemia in rats. Neurochemistry International. Advance online publication. doi: 10.1016/j.neuint.2017.07.005
LI YAN1,2, HAIXIANG GAO, CHUNZHI LI, XIAOWEN HAN and XIAOYONG QI Received August 1, 2016; Accepted September 15, 2016 DOI: 10.3892/ol.2016.5440 Effect of miR-23a on anoxia-induced phenotypic transformation of smooth muscle cells of rat pulmonary arteries and regulatory mechanism
Q: What is the approximate DAPI concentration in this solution?
A: The concentration is about 1 µg/mL.