Dog Canine BMP-5 ELISA Kit PicoKine®

BMP-5 ELISA kit for Canine

Dog Canine BMP-5 ELISA Kit PicoKine™ (96 Tests). Quantitate Dog BMP5 in cell culture supernatants, serum and bone tissue. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0310-CN
Size: 96 wells/kit, with removable strips.
Reactive Species: Canine
Application: ELISA
Sample Types: cell culture supernatants, serum and bone tissue.

Product Name

Dog Canine BMP-5 ELISA Kit PicoKine®

View all BMP-5 ELISA kits

SKU/Catalog Number

EK0310-CN

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Dog Canine BMP-5 ELISA Kit PicoKine™ (96 Tests). Quantitate Dog BMP5 in cell culture supernatants, serum and bone tissue. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Dog Canine BMP-5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0310-CN)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A317-H454

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0310-CN for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0310-CN is reactive to BMP5 in Canine samples

Validated Sample Types

cell culture supernatants, serum and bone tissue.

Application Guarantee

EK0310-CN is guaranteed for ELISA in Canine by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of BMP-5

Bone morphogenetic protein 5 is a protein that in Dog Canineas is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on Dog Caninea chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.

Kit Components

Catalog Number Description Quantity
EK0310-CN-CAP Anti-Canine BMP5 Pre-coated 96-well strip microplate 1
EK0310-CN-ST Canine BMP5 Standard 2 vials, 10 ng/tube
EK0310-CN-DA Canine BMP5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0560.0990.1160.2010.3480.6341.1351.946

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Dog Canine BMP-5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (ng/ml)1.233.466.921.383.576.85
Standard deviation0.0590.1800.3880.0730.2030.425
CV (%)4.85.25.65.35.76.2

Gene/Protein Information For BMP5 (Source: Uniprot.Org, NCBI)

Gene Name

BMP5

Full Name

Bone morphogenetic protein 5

Weight

51.737kDa

Superfamily

TGF-beta family

Alternative Names

BMP5; BMP-5; bone morphogenetic protein 5; MGC34244 BMP5 bone morphogenetic protein 5 bone morphogenetic protein 5

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BMP5, check out the BMP5 Infographic

BMP5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BMP5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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6 Customer Q&As for Dog Canine BMP-5 ELISA Kit PicoKine®

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-06-04

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-06-04

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

G. Wang

Verified customer

Asked: 2018-12-04

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-12-04

Question

Q: Can BMP-5 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-11-10

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-11-10

Question

Q: if the enzyme conjugated BMP-5 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BMP-5 antigen?

M. Edwards

Verified customer

Asked: 2018-10-20

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-10-20

Question

Q: how can I thaw whole blood sample for BMP-5 ELISA after freezing?

P. Allen

Verified customer

Asked: 2018-08-31

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BMP-5 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-08-31

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

R. Kulkarni

Verified customer

Asked: 2018-01-12

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-01-12

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