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SKUAR1142
Pack size 1mL X 6 (sufficient to make 24mL of protein solution)
Description Boster’s Dual Color Protein Loading Buffer 4X is a complete solution for the preparation of protein samples prior to SDS-PAGE and monitoring protein transfer during Western blotting.

Overview

Product Name Dual Color Protein Loading Buffer 4X
SKU/Catalog Number AR1142
Form Supplied Concentrated solution (4X)
Size 1mL X 6 (sufficient to make 24mL of protein solution)
Contents 1 X solution contains 100mM DTT, 2% SDS, 10% Glycerin, 50mM Tris, 250mM EDTA, 0.0125% Bromophenol blue, 0.0075% Pyronin Y
Storage Upon receipt store at -20°C. It is stable for one year. Product is shipped on ice.
Equivalent N/A
Application Preparation of proteins for SDS- polyacrylamide gel electrophoresis (SDS-PAGE). Monitoring protein transfer during Western blotting.
*Our Boster Guarantee covers the use of this product in the above tested applications.
Cite This Product Dual Color Protein Loading Buffer 4X (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR1142)

Introduction

Dual Color Protein Loading Buffer is a complete buffer solution system. This optimized composition of the loading buffer prevents protein degradation during sample heating prior to SDS-PAGE and electrophoresis, as well against pH changes during the SDS-PAGE run (Many proteins are sensitive to pH changes that result from temperature fluctuations of Tris buffers during electrophoresis). It contains two tracking dyes: blue (bromophenol blue) for tracking the progress of electrophoresis and pink (pyronin Y) for monitoring of protein transfer to the membrane during Western blotting procedures. Dual Color Protein Loading Buffer also contains SDS and DTT for complete disruption of all high-order protein structures.

Features

•It prevents protein degradation during sample heating prior to SDS-PAGE and electrophoresis, as well against pH changes during the SDS-PAGE run.

•It is very convenient to use such buffer for containing two kinds of dyes (blue and pink). They can be used for tracking the progress of electrophoresis and monitoring of protein transfer to the membrane during Western blotting procedures.

Protocol

1. Mix sample with Dual Color Protein Loading Buffer 4X at the ratio 3:1 (i.e. add 3g sample to 1ml Dual Color Protein Loading Buffer). Denaturize at 100°C for 3-5 min. Then load and electrophorese.
2. Stop electrophoresis when dye arrives at the bottom of gels.

Notes

1.If SDS sediments appeared when balance Dual Color Protein Loading Buffer 4X to room temperature, try to melt it with warm water.
2.Please operate with gloves in fume hood.

Dual Color protein Loading Buffer 4X Images

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Dual Color protein Loading Buffer 4X
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