ECL Detection Kit, Including HRP-Conjugated Goat Anti-Rabbit IgG Antibody

ECL Detection Kit, Including HRP-Conjugated Goat Anti-Rabbit IgG Antibody. 96wells/kit, with removable strips. Cited in 49 publication(s).

Product Info Summary

SKUEK1002
Pack size 1 kit (for 1000 cm² membrane)
Applications Western blot

Overview

Product Name ECL Detection Kit, Including HRP-Conjugated Goat Anti-Rabbit IgG Antibody
Synonyms ECL Anti-Rabbit Antibody Detection Reagent System; ECL Western Blotting Substrate Reagent Kit (Anti-Rabbit IgG); ECL-WB Substrate Reagent System (Anti-Rabbit IgG); Enhanced Chemiluminescent Western Blot Related HRP-Conjugated Goat Anti-Rabbit IgG Assay System
Description ECL Detection Kit, Including HRP-Conjugated Goat Anti-Rabbit IgG Antibody is a four-component system for sensitive detection of membrane-immobilized proteins on western blots probed with rabbit primary antibodies using HRP-conjugated anti-rabbit secondary antibodies and enhanced chemiluminescent peroxidase substrate reagents.
Application Western blot
Pack Size 1 kit (for 1000 cm² membrane)
Assays Per Kit Sufficient for covering up to 1000 cm² of PVDF or nitrocellulose membrane
Reagent TypeProtein assay kit; ECL Western Blotting related

Kit Components*

Kit Component Component Description Size
Blocking buffer Nonfat dry milk protein powder 10 g
HRP-conjugated secondary antibody, affinity purified, solid phase adsorbed HRP-conjugated goat anti-rabbit IgG 0.1ml
Enhanced chemiluminescent (ECL) reagent A HRP chemiluminescent substrate: luminol-based; enhancer 5 ml
Enhanced chemiluminescent (ECL) reagent B chemiluminescent substrate oxidizing agent 5 ml

*Additional components can be purchased. If you need extra of the above components please order them together to avoid additional shipping charges.

Assay Information

Sample Type SDS-PAGE separated-, membrane-immobilized-, primary-antibody-probed proteins from cell/tissue lysates
Assay Type Immunoassay
Assay Purpose Detect target protein; Quantify target protein level
Technique Immunodetection of target protein by HRP-conjugated secondary antibody
Detection Method Chemiluminescent
Emission 428 nm, blue glow
Equipment Needed WB instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager
Assay Sensitivity Femtomole and picogram protein quantities

Properties

Form Supplied Blocking buffer: Dry powder
HRP-Antibody conjugate: Concentrated stock solution, 0.1 mg/ml, in 0.01M PBS (pH7.4) and 50% glycerol
ECL reagents A and B: Concentrated stock solutions, 20x
Recommended Working Concentration See protocol in Datasheet
Storage 4˚C for one year
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE

Main Advantages

Specific High signal-to-noise ratio
Sensitive Up to 10 times more sensitive than colorimetric methods
Quantitative Linear response in the range 0.2 to 2 OD units
Stable The light output signal remains detectable up to several hours after incubation
Reliable Possibility for repeated exposure to X-ray film until obtain optimal results
Storable Immediate hard copy data - avoid fading of results
Reuseable Allowing reprobing on membranes up to 10 times - saves time and materials
Fast Short reaction times; few sec to few min of exposure enough to detect signal
Non-radioactive and Non-toxic No specialized facilities needed

Background

Chemiluminescence refers to the light emitted by a chemical reaction and chemiluminescent systems have emerged as the best all-around technique for the revelation step of blotting-based analyses. Horseradish Peroxidase (HRP) Conjugated Secondary Antibodies are the most popular antibody conjugates and are widely used to obtain chemiluminescent results in western and other immunoblotting, ELISA, and immunochemistry. In the chemiluminescence reaction Horseradish peroxidase (HRP) catalyzes the oxidation of cyclic diacylhydrazides, such as luminol, or acridine derivatives in the presence of hydrogen peroxide (H2O2) acting as activator or oxidizing agent. Immediately following the oxidation, the luminol is in an excited state (intermediate reaction product), which decays to the ground state by emitting light. Strong up to 1000-fold enhancement of the light emission is produced by enhancers, such as phenolic compounds, making the light emission much prolonged and very intense. This allows detection of protein at a level of 1-10 pg.

Enhanced Chemiluminescent (ECL) Reagent Kit is an immunodetection system that utilizes horseradish peroxidase (HRP) conjugated anti-mouse, anti-rabbit, anti-goat, or anti-rat secondary antibodies for ECL western blot detection of proteins. Proteins from various samples such as cell lysates are first separated within an acrylamide gel under adapted electric current (electrophoresis) and then transferred onto a PVDF or nitrocellulose membrane (transfer). The membrane containing the proteins is then incubated with specific primary antibodies which bind to their specific target (s) (capture) and subsequently with Horseradish Peroxidase (HRP)-conjugated secondary antibodies which bind to the primary antibodies (detection). Addition of chemiluminescent reagents (HRP luminol-based substrate, enhancer and activator) results in a chemiluminescent signal that can be captured using photographic or other imaging methods (revelation). The peroxidase-catalyzed oxidation of luminol produces a glow-type light emission at 428 nm. The intensity of light output is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting and thus of the amount of target antigen.

Product Images

EK1002 has been cited in 49 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chao Hu,Xiaobin Zhu,Taogen Zhang,Zhouming Deng,Yuanlong Xie,Feifei Yan,Lin Cai," Tanshinone IIA Inhibits Osteosarcoma Growth through a Src Kinase-Dependent Mechanism",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 5563691,15 pages,2021.https://doi. org/10.1155/2021/5563691
Species: Human,Mouse
EK1002 usage in article: APP:WB, SAMPLE:U2-OS CELL AND MG-63 CELL, DILUTION:NA

Lv M,Xue G,Cheng H,Meng P,Lian X,Hölscher C,Li D. The GLP-1/GIP dual-receptor agonist DA5-CH inhibits the NF-κB inflammatory pathway in the MPTP mouse model of Parkinson‘s disease more effectively than the GLP-1 single-receptor agonist NLY01. Brain Behav. 2021 Jun 14. doi: 10.1002/brb3.2231. Epub ahead of print. PMID: 34125470.
Species: Mouse
EK1002 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA

Tang J,Wu Z,Tian Z,Chen W,Wu G.OTUD7B stabilizes estrogen receptor α and promotes breast cancer cell proliferation.Cell Death Dis.2021 May 25;12(6):534.doi:10. 1038/s41419-021-03785-7.PMID:34035221;PMCID:PMC8149656.
Species: Human,Mouse
EK1002 usage in article: APP:WB, SAMPLE:HEK293 CELL, DILUTION:NA

Zhang F,Chen L,Zhou Y,Ding D,Hu Q,Liu Y,Li K,Wu S,He L,Lei M,Du R.Dexamethasone prevents the Epstein-Barr virus induced epithelial-mesenchymal transition in A549 cells.J Med Virol.2020 May 12.doi:10.1002/jmv.25999.Epub ahead of print.PMID:32396272.
Species: Human
EK1002 usage in article: APP:WB, SAMPLE:A549 CELL, DILUTION:NA

Lu X,Liang B,Li S,Chen Z,Chang W.Modulation of HOXA9 after skeletal muscle denervation and reinnervation.Am J Physiol Cell Physiol.2020 Jun 1;318(6):C1154-C1165.doi:10.1152/ajpcell.00055.2020.Epub 2020 Apr 1.PMID:32233950.
Species: Rat
EK1002 usage in article: APP:WB, SAMPLE:GASTROCNEMIUS MUSCLE, DILUTION:NA

Lian,L.,Xu,H.,Zhang,H. et al.Overexpression of OsSPL14 results in transcriptome and physiology changes in indica rice ‘MH86’.Plant Growth Regul 90,265–278 (2020). https://doi.org/10.1007/s10725-019-00569-0
Species: Rice plant
EK1002 usage in article: APP:WB, SAMPLE:SEED, DILUTION:NA

Yang,P.,Li,Z.,Tye,K.D. et al.Effects of an orally supplemented probiotic on the autophagy protein LC3 and Beclin1 in placentas undergoing spontaneous delivery during normal pregnancy.BMC Pregnancy Childbirth 20,216 (2020).https://doi.org/10.1186/s12884-020-02905-z
Species: Human
EK1002 usage in article: APP:WB, SAMPLE:PLACENTAL TISSUE, DILUTION:NA

Dan H,Lei X,Huang X,Ma N,Xing Y,Shen Y. CM082, a novel VEGF receptor tyrosine kinase inhibitor, can inhibit angiogenesis in vitro and in vivo. Microvasc Res. 2021 Feb 19;136:104146. doi: 10.1016/j.mvr.2021.104146. Epub ahead of print.PMID:33610563.
Species: Human,Mouse
EK1002 usage in article: APP:WB, SAMPLE:HUVECS, DILUTION:NA

Bi,F.,Chen,C.,Fu,J.,Yu,L.,& Geng,J.(2021).Inhibiting proliferation and metastasis of osteosarcoma cells by downregulation of long non‑coding RNA colon cancer‑associated transcript 2 targeting microRNA‑143.Oncology Letters,21,265.https://doi.org/10.3892/ol
Species: Human
EK1002 usage in article: APP:WB, SAMPLE:MG63 CELL AND U2OS CELL, DILUTION:1:5000

Zhihua Zhang,Wenping Gao,Xiaojian Wang,Di Zhang,Yueze Liu,XiaoHui Wang,Lin Li. mTOR-Autophagy: A Key for Geniposide Protection in APP/PS1 Transgenic Mice. In: Prime Archives in Aging. Hyderabad, India: Vide Leaf. 2020.
Species: Mouse
EK1002 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS AND CORTEX, DILUTION:NA

Yang QQ,Li HN,Zhang ST,Yu YL,Wei W,Zhang X,Wang JY,Zeng XY.Red nucleus IL-6 mediates the maintenance of neuropathic pain by inducing the productions of TNF-α and IL-1β through the JAK2/STAT3 and ERK signaling pathways.Neuropathology.2020 Aug;40(4):347-357
Species: Rat
EK1002 usage in article: APP:WB, SAMPLE:RN TISSUE, DILUTION:NA

Zhao H,Li R,Wang X,Lu X,Hu M,Zhang J,Zhao X,Song X,Liu Y. The role of apatinib combined with paclitaxel (aluminum binding type) in platinum-resistant ovarian cancer. J Ovarian Res.2020 Sep 21;13(1):113. doi:10.1186/s13048-020-00719-3.PMID: 32958014;PMCID:
Species: Human,Mouse
EK1002 usage in article: APP:WB, SAMPLE:SKOV-3/DDP CELL, DILUTION:NA

Xiao N, Li C, Liao W, Yin J, Zhang S, Zhang P, Yuan L, Hong M. FOXG1 mediates the radiosensitivity of glioma cells through regulation of autophagy. Int J Radiat Biol. 2020 Nov 17:1-32. doi:10.1080/09553002.2021.18 46816. Epub ahead of print. PMID: 3320174
Species: Human
EK1002 usage in article: APP:WB, SAMPLE:U87 MG CELLS, DILUTION:NA

Kong XX, Jiang S, Liu T, Liu GF, Dong M. Paclitaxel increases sensitivity of SKOV3 cells to hyperthermia by inhibiting heat shock protein 27. Biomed Pharmacother. 2020 Oct 24;132:110907.doi: 10.1016/j.biopha.2020. 110907. Epub ahead of print. PMID: 331134
Species: Human
EK1002 usage in article: APP:WB, SAMPLE:SKOV3 CELLS, DILUTION:NA

Integrin-?1, not integrin-?5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain

Wang Wq, Zhang H, Wang Hb, Sun Yg, Peng Zh, Zhou G, Yang Sm, Wang Rq, Fang Dc. Mol Diagn Ther. 2010 Jun 1;14(3):155-61. Doi: 10.2165/11536340-000000000-00000. Programmed Cell Death 4 (Pdcd4) Enhances The Sensitivity Of Gastric Cancer Cells To Trai...

Wang Ph, Gu Zh, Wan Dh, Zhu Wb, Qiu W, Weng Sp, Yu Xq, He Jg. Plos One. 2013;8(2):E52088. Doi: 10.1371/Journal.Pone.0052088. Epub 2013 Feb 6. Litopenaeus Vannamei Sterile-Alpha And Armadillo Motif Containing Protein (Lvsarm) Is Involved In Regulat...

Li Ss, Shen Js, Ren Dx, Liu Jx. Animal. 2015 Feb;9(2):267-74. Doi: 10.1017/S1751731114002225. Epub 2014 Sep 17. Effects Of The Processing Methods Of Corn Grain And Soybean Meal On Milk Protein Expression Profiles In Dairy Cows.

Yang S, Yu M, Sun L, Xiao W, Yang X, Sun L, Zhang C, Ma Y, Yang H, Liu Y, Lu D, Teitelbaum Dh, Yang H. J Interferon Cytokine Res. 2014 Mar;34(3):195-203. Doi: 10.1089/Jir.2013.0044. Epub 2013 Nov 15. Interferon-??-Induced Intestinal Epithelial Bar...

Wang Tb, Hu Bg, Liu Dw, Shi Hp, Dong Wg. Int J Oncol. 2014 Jun;44(6):1861-9. Doi: 10.3892/Ijo.2014.2348. Epub 2014 Mar 19. The Influence Of Lentivirus-Mediated Cxcr4 Rna Interference On Hepatic Metastasis Of Colorectal Cancer.

Sun Z, Niu R, Su K, Wang B, Wang J, Zhang J, Wang J. Arch Toxicol. 2010 May;84(5):353-61. Doi: 10.1007/S00204-009-0508-X. Epub 2010 Jan 6. Effects Of Sodium Fluoride On Hyperactivation And Ca2+ Signaling Pathway In Sperm From Mice: An In Vivo Study.

Wang B, Xu Yf, He Bs, Pan Yq, Zhang Lr, Zhu C, Qu Ll, Wang Sk. J Exp Clin Cancer Res. 2010 Jun 3;29:61. Doi: 10.1186/1756-9966-29-61. Rnai-Mediated Silencing Of Cd147 Inhibits Tumor Cell Proliferation, Invasion And Increases Chemosensitivity To Ci...

Cao X, Ma J, Wu G, Zhang C, Wang L, Dai S, Xu W. Neurochem Int. 2012 Jan;60(2):163-9. Doi: 10.1016/J.Neuint.2011.05.005. Epub 2011 Jun 6. Thymus-Expressed Chemokine Promotes Survival Of Pc12 Cells Via Pi3K Pathway.

Wang Ws, Liang Hy, Cai Yj, Yang H. J Interferon Cytokine Res. 2014 Jan;34(1):60-9. Doi: 10.1089/Jir.2013.0040. Epub 2013 Sep 6. Dmog Ameliorates Ifn-??-Induced Intestinal Barrier Dysfunction By Suppressing Phd2-Dependent Hif-1?? Degradation.

Li R, Pan Y, He B, Xu Y, Gao T, Song G, Sun H, Deng Q, Wang S. Int J Oncol. 2013 Dec;43(6):1885-94. Doi: 10.3892/Ijo.2013.2108. Epub 2013 Sep 23. Downregulation Of Cd147 Expression By Rna Interference Inhibits Ht29 Cell Proliferation, Invasion And...

Yu J, Lu Y, Cui D, Li E, Zhu Y, Zhao Y, Zhao F, Xia S. Oncol Rep. 2014 Feb;31(2):910-8. Doi: 10.3892/Or.2013.2897. Epub 2013 Dec 5. Mir-200B Suppresses Cell Proliferation, Migration And Enhances Chemosensitivity In Prostate Cancer By Regulating Bm...

Meng Z, Shi Zr, Tan Gz, Yin J, Wu J, Mi Xb, Wang L. Lupus. 2014 Feb;23(2):183-7. Doi: 10.1177/0961203313513820. Epub 2013 Dec 3. The Association Of Anti-Annexin1 Antibodies With The Occurrence Of Skin Lesions In Systemic Lupus Erythematosus.

Li Q, Mao L, Wang R, Zhu L, Xue L. Mol Biol Rep. 2014;41(4):2409-17. Doi: 10.1007/S11033-014-3095-8. Epub 2014 Jan 16. Overexpression Of S-Adenosylhomocysteine Hydrolase (Sahh) In Esophageal Squamous Cell Carcinoma (Escc) Cell Lines: Effects On Ap...

Wang Q, Wang Y, Du L, Xu C, Sun Y, Yang B, Sun Z, Fu Y, Cai L, Fan S, Fan F, Liu Q. Int J Mol Sci. 2014 Jan 29;15(2):2157-71. Doi: 10.3390/Ijms15022157. Shrna-Mediated Xrcc2 Gene Knockdown Efficiently Sensitizes Colon Tumor Cells To X-Ray Irradiat...

Li Y, Yang D, Wang Jy, Yao Y, Zhang Wz, Wang Lj, Cheng Dc, Yang Fk, Zhang Fm, Zhuang M, Ling H. Plos One. 2014 Jan 21;9(1):E86083. Doi: 10.1371/Journal.Pone.0086083. Ecollection 2014. Critical Amino Acids Within The Human Immunodeficiency Virus Ty...

Zhong L, Yuan L, Rao Y, Li Z, Zhang X, Liao T, Xu Y, Dai H. Aquat Toxicol. 2014 Apr;149:1-7. Doi: 10.1016/J.Aquatox.2014.01.022. Epub 2014 Feb 2. Distribution Of Vitellogenin In Zebrafish (Danio Rerio) Tissues For Biomarker Analysis.

Yu Jj, Wu Yx, Zhao Fj, Xia Sj. Med Oncol. 2014 Apr;31(4):910. Doi: 10.1007/S12032-014-0910-Y. Epub 2014 Mar 15. Mir-96 Promotes Cell Proliferation And Clonogenicity By Down-Regulating Of Foxo1 In Prostate Cancer Cells.

Wu Y, Yu J, Liu Y, Yuan L, Yan H, Jing J, Xu G. Int J Mol Med. 2014 Jun;33(6):1563-9. Doi: 10.3892/Ijmm.2014.1724. Epub 2014 Apr 4. Delivery Of Ezh2-Shrna With Mpeg-Pei Nanoparticles For The Treatment Of Prostate Cancer In Vitro.

Yang Y, Qiu Y, Wang W, Xiao W, Liang H, Zhang C, Yang H, Teitelbaum Dh, Sun Lh, Yang H. Int J Clin Exp Pathol. 2014 Apr 15;7(5):2006-18. Ecollection 2014. Adenosine A2B Receptor Modulates Intestinal Barrier Function Under Hypoxic And Ischemia/Repe...

Zhang W, Shen Zy, Song Hl, Yang Y, Wu Bj, Fu Nn, Liu T. World J Gastroenterol. 2014 Jun 21;20(23):7442-51. Doi: 10.3748/Wjg.V20.I23.7442. Protective Effect Of Bone Marrow Mesenchymal Stem Cells In Intestinal Barrier Permeability After Heterotopic ...

Zhang X. Tumour Biol. 2014 Nov;35(11):10781-8. Doi: 10.1007/S13277-014-2090-Y. Epub 2014 Jul 31. Depression Of Testes-Specific Protease 50 (Tsp50) Inhibits Cell Proliferation And Induces Apoptosis In Laryngocarcinoma.

Zhong L, Yuan L, Rao Y, Li Z, Gu Q, Long Y, Zhang X, Cui Z, Xu Y, Dai H. Aquat Toxicol. 2014 Nov;156:1-9. Doi: 10.1016/J.Aquatox.2014.07.016. Epub 2014 Jul 29. Investigation Of Effect Of 17??-Ethinylestradiol On Vigilin Expression Using An Isolate...

Sun H, Pan Y, He B, Deng Q, Li R, Xu Y, Chen J, Gao T, Ying H, Wang F, Liu X, Wang S. Int J Oncol. 2015 Apr;46(4):1759-67. Doi: 10.3892/Ijo.2015.2852. Epub 2015 Jan 26. Gene Therapy For Human Colorectal Cancer Cell Lines With Recombinant Adenoviru...

Zhou X, Li Yj, Gao Sy, Wang Xz, Wang Py, Yan Yf, Xie Sy, Lv Cj. J Cell Mol Med. 2015 May;19(5):1103-13. Doi: 10.1111/Jcmm.12506. Epub 2015 Feb 20. Sulindac Has Strong Antifibrotic Effects By Suppressing Stat3-Related Mir-21.

Hu J, Pan J, Luo Z, Tao Z. Cell Biochem Biophys. 2015 Feb 21. [Epub Ahead Of Print] Downregulation Of Survivin By Shrna Inhibits Invasion And Enhances The Radiosensitivity Of Laryngeal Squamous Cell Carcinoma.

Yu M, Yang S, Qiu Y, Chen G, Wang W, Xu C, Cai W, Sun L, Xiao W, Yang H. J Gastroenterol. 2015 Nov;50(11):1103-13. Doi: 10.1007/S00535-015-1066-Z. Epub 2015 Mar 28. Par-3 Modulates Intestinal Epithelial Barrier Function Through Regulating Intracel...

Chen N, Wu L, Yuan H, Wang J. Int J Biol Sci. 2015 May 30;11(7):833-44. Doi: 10.7150/Ijbs.10564. Ecollection 2015. Ros/Autophagy/Nrf2 Pathway Mediated Low-Dose Radiation Induced Radio-Resistance In Human Lung Adenocarcinoma A549 Cell.

Jin H, Dong Yy, Zhang H, Cui Y, Xie K, Lou G. Oncol Res. 2015;22(3):167-76. Doi: 10.3727/096504015X14298122915664. Shrna Depletion Of Ciap1 Sensitizes Human Ovarian Cancer Cells To Anticancer Agent-Induced Apoptosis.

Wang J, Xie Y, Feng Y, Zhang L, Huang X, Shen X, Luo X. Int J Oncol. 2015 Apr;46(4):1507-15. Doi: 10.3892/Ijo.2015.2869. Epub 2015 Feb 3. (-)-Epigallocatechingallate Induces Apoptosis In B Lymphoma Cells Via Caspase-Dependent Pathway And Bcl-2 Fam...

Tao T, Zhao Z, Hao L, Gu M, Chen L, Tang J. J Neurosurg Anesthesiol. 2013 Jul;25(3):299-305. Doi: 10.1097/Ana.0B013E31828Baa93. Propofol Promotes Proliferation Of Cultured Adult Rat Hippocampal Neural Stem Cells.

Sun Z, Niu R, Wang B, Jiao Z, Wang J, Zhang J, Wang S, Wang J. Arch Toxicol. 2011 Nov;85(11):1441-52. Doi: 10.1007/S00204-011-0672-7. Epub 2011 Feb 22. Fluoride-Induced Apoptosis And Gene Expression Profiling In Mice Sperm In Vivo.

Yu D, Rui X, He S. Vasc Endovascular Surg. 2014 May;48(4):289-96. Doi: 10.1177/1538574413520518. Epub 2014 Jan 27. Effect Of Heparin-Derived Oligosaccharide On Bfgfr1 And Bfgfr2 In Vascular Smooth Muscle Cells.

Ma Z, Tao L, Bechthold A, Shentu X, Bian Y, Yu X. Appl Microbiol Biotechnol. 2014 Jun;98(11):5051-8. Doi: 10.1007/S00253-014-5573-2. Epub 2014 Feb 9. Overexpression Of Ribosome Recycling Factor Is Responsible For Improvement Of Nucleotide Antibiot...

Huang Y, Li X, Wang Y, Wang H, Huang C, Li J. Mol Cell Biochem. 2014 Sep;394(1-2):1-12. Doi: 10.1007/S11010-014-2073-8. Epub 2014 Jun 25. Endoplasmic Reticulum Stress-Induced Hepatic Stellate Cell Apoptosis Through Calcium-Mediated Jnk/P38 Mapk An...

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