FIR Colorimetric Cell-Based ELISA

FARP2 ELISA kit, cell-based

The FIR Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FIR protein expression profile in cells. The kit can be used for measuring the relative amounts of FIR in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FIR.

Product Info Summary

SKU: EKC1656
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

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Product Name

FIR Colorimetric Cell-Based ELISA

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SKU/Catalog Number

EKC1656

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The FIR Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FIR protein expression profile in cells. The kit can be used for measuring the relative amounts of FIR in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FIR.

Storage & Handling

4°C/6 Months

Cite This Product

FIR Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1656)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1656 is reactive to FARP2 in Human, Mouse samples

Application Guarantee

EKC1656 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-FARP2 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For FARP2 (Source: Uniprot.Org, NCBI)

Uniprot ID

O94887

Gene ID

9855

Gene Name

FARP2

Full Name

FERM, ARHGEF and pleckstrin domain-containing protein 2

Weight

119.888kDa

Alternative Names

FERM, RhoGEF and pleckstrin domain protein 2; FGD1-related Cdc42-GEF; FIRFERM domain including RhoGEF; FRG; KIAA0793PH domain-containing family C member 3; Pleckstrin homology domain-containing family C member 3; PLEKHC3FERM, RhoGEF and pleckstrin domain-containing protein 2 FARP2 FIR, FRG, PLEKHC3 FERM, ARH/RhoGEF and pleckstrin domain protein 2 FERM, ARHGEF and pleckstrin domain-containing protein 2|FERM domain including RhoGEF|FERM, RhoGEF and pleckstrin domain protein 2|FERM, RhoGEF and pleckstrin domain-containing protein 2|FGD1-related Cdc42-GEF|PH domain-containing family C member 3|pleckstrin homology domain-containing family C member 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FARP2, check out the FARP2 Infographic

FARP2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FARP2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

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