Show Order Info
SKU BA1101
Pack size 0.5 ml
Applications Flow Cytometry, IHC, ICC
Price: $80.00

Product Overview

Product Name Goat Anti-Mouse IgG Secondary Antibody, FITC Conjugate
Synonyms FITC-conjugated Goat Anti-mouse IgG; Goat Anti-Mouse IgG-FITC Secondary Antibody; Fluorescein-labeled Goat Anti-Mouse IgG Secondary Antibody;ee
Description Goat Anti-Mouse IgG Secondary Antibody, FITC Conjugate, for detection, localization and quantification of target proteins in a sample via indirect immunofluorescence in IHC-P, IHC-F, ICC, or FCM
Reagent Type Fluorophore-conjugated secondary antibody
Label FITC
Host Goat
Target Species Mouse
Antibody Class IgG
Clonality Polyclonal
Immunogen Whole molecule mouse IgG
Purification Immunoaffinity chromatography
Solid Phase Adsorbtion Human serum proteins
Specificity Mouse IgG specific
Form Supplied No cross-reactivity with human/bovine/rabbit IgG
Formulation 0.5 mg FITC-conjugated secondary antibody
0.01 M PBS (PH 7.4)
0.01% Thimerosal
50% glycerol
Pack Size 0.5 ml
Concentration 1 mg/ml
Application Flow Cytometry (FCM), Immunohistochemistry (paraffin-embedded (IHC-P) and frozen(IHC-F) sections), Immunocytochemistry (ICC)
*Our Boster Guarantee covers the use of this product in the above marked tested applications.
Storage 4C for 1 year
Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE

Assay Information

Sample Type Cell suspension, FFPE tissue sections, thawed frozen samples
Assay Type Immunoanalytical
Assay Purpose Protein detection/quantification
Technique Immunodetection of target antibody with HRP reporter enzyme
Equipment Needed Excitation light source; Filter set and detector: fluorescence microscope (can be combined with confocal microscope), fluorescence plate-reader, flow cytometer, or cell sorter;

Main Advantages

Specific High signal-to-noise ratio
Fast Fewer number of processing steps - no need for adding a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; Fluorescence can be observed directly
High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody; Multiple FITC molecules bind to a single secondary antibody;
High Image Quality High-resolution compatibility with fluorescent microscopy; compatible with multi-planar microscopy
Precise target localization Sharp, precise signal development
Multiplex Compatibility Colocalization studies: use primary antibodies from different host species for simultaneous detection by fluorophore-conjugated secondary antibodies; use multiple differently colored fluorophores in the same experiment for target differentiation
Quantifieable Rapid and precise quantitative analysis of fluorescent signal

Background

Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.

Immunofluorescence is a technique used for light microscopy with a fluorescence microscope which utilizes fluorescent dyes as reporters. It is being employed in a variety of applications such as cellular imaging and flow cytometry and is commonly used to visualize the distribution of target molecules through a sample, to detect protein location and activation, to identify protein complex formation and conformational changes, to monitor biological processes in vivo.
Fluorescent dyes (also known as fluorochromes, fluorophores, or simply fluors) are molecules that can absorb light of a specific energy and wavelength, thereby undergoing excitation, and then re-emit it at a lower energy and longer wavelength upon returning to the ground state.

Goat Anti-Mouse IgG Secondary Antibody, FITC Conjugate Images

Click the images to enlarge.

Goat Anti-Mouse IgG Secondary Antibody, FITC Conjugate
PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti- PCK Antigen Affinity purified monoclonal antibody (Catalog # MA1081) at 1 ??g/mL. FITC Conjugated Goat Anti-mouse IgG secondary antibody (Catalog # BA1101) was used to detect the primary antibody at 30??g/mL.
Write a review for BA1101

Publications

Shen, Y., Cao, R., Liu, W., Zhou, Y., Wu, Y., Tan, J.,…, & Zu, X. (2017). Negative feedback loop between ZBTB7A and TGF-β in breast cancer. Oncology Letters. doi: 10.3892/ol.2017.6291
Liu, Z., Liu, Y., Zhang, Y., Yang, Y., Ren, J., Zhang, X., & Du, E. (2017). Surface displaying of swine IgG1 Fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction. Veterinary Research, 48(1), 29. doi: 10.1186/s13567-017-0434-5
Li, J., Luo, H., Dong, X., Liu, Q., Wu, C., Zhang, T.,…, & Li, L. (2017). Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model. Stem Cell Research & Therapy, 8(1),107. doi: 10.1186/s13287-017-0547-9
Jing Donga, Juan Baia, Tao Suna, Zhenqing Gua, Jichun Wangb, Haifeng Suna, Ping Jiang Research in Veterinary Science Volume 115, December 2017, Pages 17–23 Comparative pathogenicity and immunogenicity of triple and double gene-deletion pseudorabies virus vaccine candidates
Jing Donga, Juan Baia, Tao Suna, Zhenqing Gua, Jichun Wangb, Haifeng Suna, Ping Jiang Research in Veterinary Science Volume 115, December 2017, Pages 17–23 Comparative pathogenicity and immunogenicity of triple and double gene-deletion pseudorabies virus vaccine candidates