FLT3 Colorimetric Cell-Based ELISA Kit

Flt-3/Flk-2/CD135 ELISA kit, cell-based

The FLT3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FLT3 protein expression profile in cells. The kit can be used for measuring the relative amounts of FLT3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FLT3.

Product Info Summary

SKU: EKC1219
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA
Sample Types: Cell lines

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Product Name

FLT3 Colorimetric Cell-Based ELISA Kit

See all Flt-3/Flk-2/CD135 products

SKU/Catalog Number

EKC1219

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The FLT3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FLT3 protein expression profile in cells. The kit can be used for measuring the relative amounts of FLT3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FLT3.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

FLT3 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1219)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1219 is reactive to FLT3 in Human, Mouse samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1219 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Flt-3/Flk-2/CD135 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For FLT3 (Source: Uniprot.Org, NCBI)

Gene Name

FLT3

Full Name

Receptor-type tyrosine-protein kinase FLT3

Weight

112.903kDa

Superfamily

protein kinase superfamily

Alternative Names

CD135 antigen; CD135; EC 2.7.10; fetal liver kinase 2; FL cytokine receptor; FLK2; Flk-2; FLT3 receptor tyrosine kinase; Flt3; Flt-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; Stem cell tyrosine kinase 1; STK-1; STK1EC 2.7.10.1; Tyrosine-protein kinase receptor FLT3 FLT3 CD135, FLK-2, FLK2, STK1 fms related receptor tyrosine kinase 3 receptor-type tyrosine-protein kinase FLT3|CD135 antigen|FL cytokine receptor|fetal liver kinase 2|fms related tyrosine kinase 3|fms-like tyrosine kinase 3|growth factor receptor tyrosine kinase type III|stem cell tyrosine kinase 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FLT3, check out the FLT3 Infographic

FLT3 infographic

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In this infographic you will see the following information for FLT3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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